This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSLIK-NFLAG-hEWSR1WT-CMyc
catalog :
50733
citations: 1
Reference
Couthouis J, Hart M, Erion R, King O, Diaz Z, Nakaya T, et al. Evaluating the role of the FUS/TLS-related gene EWSR1 in amyotrophic lateral sclerosis. Hum Mol Genet. 2012;21:2899-911 pubmed publisher
product information
Catalog Number :
50733
Product Name :
pSLIK-NFLAG-hEWSR1WT-CMyc
article :
doi10.1093/hmg/dds116
id7684
pubmed_id22454397
bacterial resistance :
Ampicillin
cloning :
backbonepSLIK-neo
backbone_mutationhEWSR1 was cloned into pEN_Tmcs vector at SpeI/NotI sites. The SpeI site was blunt-ended for use. Using LR-recombination, the insert was recombinated into pSLIK-neo vector along with TRE promoter.
backbone_originIain Fraser(Addgene plasmid #25735)
backbone_size13286
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth strain :
Produces lentivirus expressing human EWSR1 wild type with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterTRE
sequencing_primer_3CCATCTTTATGGCTCGAGATG
sequencing_primer_5TGGAGACGCCATCCACGCTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEWS, EWS-FLI1, bK984G1.4
geneEWSR1
id2130
genbank_ids
NM_001163285.1
NM_001163285.1
mutation
nameEWSR1
shRNA_sequence
size2032
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
locationC terminal on insert
tagMyc
resistance markers :
1796
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA