This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGFP CrkII
catalog :
50730
citations: 1
Reference
Badu Nkansah K, Lechler T. Proteomic analysis of desmosomes reveals novel components required for epidermal integrity. Mol Biol Cell. 2020;31:1140-1153 pubmed publisher
product information
Catalog Number :
50730
Product Name :
pGFP CrkII
article :
doi
id7644
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepGZ21dxZ
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
mammalian expression of GFP-CrkII. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3GFP ASO (TTGTAACCATTATAAGCTGC)
sequencing_primer_5GFP FW (AAAGACCCCAACGAGAAGCG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCRKII, p38
geneCRK
id1398
genbank_ids
GI:1219554
mutation
nameCrkII
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGFP
plasmid copy :
cDNA was obtained from Dr. Silvio Gutkind (NIDCR, NIH).
resistance markers :
1793
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA