This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EB3-tdTomato
catalog :
50708
citations: 16
Reference
Velasco C, Santarella Mellwig R, Schorb M, Gao L, Thorn Seshold O, Llobet A. Microtubule depolymerization contributes to spontaneous neurotransmitter release in vitro. Commun Biol. 2023;6:488 pubmed publisher
Severin M, Pedersen E, Borre M, Axholm I, Christiansen F, Ponniah M, et al. Dynamic localization of the Na+-HCO3- co-transporter NBCn1 to the plasma membrane, centrosomes, spindle and primary cilia. J Cell Sci. 2023;136: pubmed publisher
Jansen K, Iwanski M, Burute M, Kapitein L. A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle. J Cell Biol. 2023;222: pubmed publisher
Castrogiovanni C, Inchingolo A, Harrison J, Dudka D, Sen O, Burroughs N, et al. Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules. Nat Commun. 2022;13:4704 pubmed publisher
Meka D, Kobler O, Hong S, Friedrich C, Wuesthoff S, Henis M, et al. Centrosome-dependent microtubule modifications set the conditions for axon formation. Cell Rep. 2022;39:110686 pubmed publisher
Shams H, Matsunaga A, Ma Q, Mofrad M, Didonna A. Methylation at a conserved lysine residue modulates tau assembly and cellular functions. Mol Cell Neurosci. 2022;120:103707 pubmed publisher
Peris L, Parato J, Qu X, Soleilhac J, Lanté F, Kumar A, et al. Tubulin tyrosination regulates synaptic function and is disrupted in Alzheimer's disease. Brain. 2022;: pubmed publisher
Aghaizu N, Warre Cornish K, Robinson M, Waldron P, Maswood R, Smith A, et al. Repeated nuclear translocations underlie photoreceptor positioning and lamination of the outer nuclear layer in the mammalian retina. Cell Rep. 2021;36:109461 pubmed publisher
Jiang X, Ho D, Mahe K, Mia J, Sepúlveda G, Antkowiak M, et al. Condensation of pericentrin proteins in human cells illuminates phase separation in centrosome assembly. J Cell Sci. 2021;134: pubmed publisher
Müller Deku A, Meiring J, Loy K, Kraus Y, Heise C, Bingham R, et al. Photoswitchable paclitaxel-based microtubule stabilisers allow optical control over the microtubule cytoskeleton. Nat Commun. 2020;11:4640 pubmed publisher
Movsisyan N, Pardo L. Kv10.1 Regulates Microtubule Dynamics during Mitosis. Cancers (Basel). 2020;12: pubmed publisher
Baltussen L, Negraes P, Silvestre M, Claxton S, Moeskops M, Christodoulou E, et al. Chemical genetic identification of CDKL5 substrates reveals its role in neuronal microtubule dynamics. EMBO J. 2018;37: pubmed publisher
Zenker J, White M, Gasnier M, Alvarez Y, Lim H, Bissiere S, et al. Expanding Actin Rings Zipper the Mouse Embryo for Blastocyst Formation. Cell. 2018;173:776-791.e17 pubmed publisher
Biswas S, Kalil K. The Microtubule-Associated Protein Tau Mediates the Organization of Microtubules and Their Dynamic Exploration of Actin-Rich Lamellipodia and Filopodia of Cortical Growth Cones. J Neurosci. 2018;38:291-307 pubmed publisher
Kern D, Nicholls P, Page D, Cheeseman I. A mitotic SKAP isoform regulates spindle positioning at astral microtubule plus ends. J Cell Biol. 2016;213:315-28 pubmed publisher
Merriam E, Millette M, Lumbard D, Saengsawang W, Fothergill T, Hu X, et al. Synaptic regulation of microtubule dynamics in dendritic spines by calcium, F-actin, and drebrin. J Neurosci. 2013;33:16471-82 pubmed publisher
product information
Catalog Number :
50708
Product Name :
EB3-tdTomato
article :
doi10.1523/JNEUROSCI.0661-13.2013
id7675
pubmed_id24133252
bacterial resistance :
Kanamycin
cloning :
backbonetdTomato-N1
backbone_mutation
backbone_originClontech
backbone_size5443
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
We received the EB3 construct from Niels Galjart (Erasmus U, Netherlands) originally and replaced GFP with tdTomato. There is a Sal I site at the 5' end of EB3 and a Not I site at the 3' end of tdTomato.
growth strain :
expresses EB3-tdTomato fusion in mammalian cells for labeling the growing tips of MTs
origin :
37
pi :
alt_names
MAPRE3
cloning
clone_methodRestriction Enzyme
cloning_site_3unknown
cloning_site_5Sal1
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEB3, EBF3, EBF3-S, RP3
geneMAPRE3
id22924
genbank_ids
mutation
nameEB3
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagtdTomato
resistance markers :
1800
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA