This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAFW-Ago2
catalog :
50554
citations: 1
Reference
Iwasaki S, Kobayashi M, Yoda M, Sakaguchi Y, Katsuma S, Suzuki T, et al. Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes. Mol Cell. 2010;39:292-9 pubmed publisher
product information
Catalog Number :
50554
Product Name :
pAFW-Ago2
article :
doi10.1016/j.molcel.2010.05.015
id7652
pubmed_id20605501
bacterial resistance :
Ampicillin
cloning :
backbonepAFW
backbone_mutation
backbone_originThe Drosophila Gateway Vector Collection
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
Please note that when compared to GenBank reference sequence NP_648775.1, the Ago2 sequence in this plasmid is missing amino acids 49-54 (LQQPQQ).
growth strain :
Expression of FLAG tagged Ago2 in S2 cell
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterActin5C
sequencing_primer_3
sequencing_primer_5AC5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG7439, AG02, AGO 2, AGO1, Ago-2, Ago2, Ago2a, CG13452, CG7439, Dm Ago2, DmAgo2, Dmel CG7439, ago, ago-2, ago2, argonaute2, dAGO2, dAgo2, eIF2C2
geneAGO2
id39683
genbank_ids
mutation
nameArgonaute2
shRNA_sequence
size
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagFLAG
plasmid copy :
Gene origin is from Phillip Zamore at Univ. of Massachusetts.
resistance markers :
529
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA