This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAFW-Ago1
catalog :
50553
citations: 1
Reference
Kawamata T, Seitz H, Tomari Y. Structural determinants of miRNAs for RISC loading and slicer-independent unwinding. Nat Struct Mol Biol. 2009;16:953-60 pubmed publisher
product information
Catalog Number :
50553
Product Name :
pAFW-Ago1
article :
doi10.1038/nsmb.1630
id7651
pubmed_id19684602
bacterial resistance :
Ampicillin
cloning :
backbonepAFW
backbone_mutation
backbone_originThe Drosophila Gateway Vector Collection
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
The closest reference sequence for the Ago1 insert in this plasmid appears to be NM_079010.4, Argonaute-1, isoform B.
growth strain :
Expression of FLAG tagged Ago1 in S2 cell
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterActin5C
sequencing_primer_3
sequencing_primer_5AC5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG6671, AGO-1, Ago, Ago-1, Ago1, Agp1, CG6671, Dm Ago1, DmAgo1, Dmel CG6671, MRE20, ago1, ago1-1, anon-WO0257455.29, argonaute1, dAGO1, dAgo1, dmAgo1, l(2)04845, l(2)4845, l(2)k00208, l(2)k08121
geneAGO1
id36544
genbank_ids
mutation
nameArgonaute1
shRNA_sequence
size
species
7227
Drosophila melanogaster
tags
locationN terminal on backbone
tagFLAG
plasmid copy :
Gene origin is from Phillip Zamore at Univ. of Massachusetts.
resistance markers :
529
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA