This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDNA3.1-nAChr-beta3-CFP
catalog :
50485
citations: 1
Reference
Drenan R, Nashmi R, Imoukhuede P, Just H, McKinney S, Lester H. Subcellular trafficking, pentameric assembly, and subunit stoichiometry of neuronal nicotinic acetylcholine receptors containing fluorescently labeled alpha6 and beta3 subunits. Mol Pharmacol. 2008;73:27-41 pubmed
product information
Catalog Number :
50485
Product Name :
pCDNA3.1-nAChr-beta3-CFP
article :
doi10.1124/mol.107.039180
id7336
pubmed_id17932221
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originLife Technologies
backbone_size5597
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
origin :
37
pi :
alt_names
nAchR beta3-CFP
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRV
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed1
entrez_gene
aliases5730417K16Rik, Acrb3
geneChrnb3
id108043
genbank_ids
AAL75573.1
AF467896_1
mutationCFP fusion in M3-M4 loop (after residue P379). Our CFP fusion cassette also consisted of a Gly-Ala-Gly flexible linker flanking the CFP open reading frame on both N and C terminal sides
namenAChRBeta3 subunit, isoform 1
shRNA_sequence
size2230
species
10090
Mus musculus
tags
location
tagCFP
resistance markers :
288
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA