pcDNA3.1-nAChr-Alpha6-CFP | Addgene 50482 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.1-nAChr-Alpha6-CFP

catalog :

50482

citations: 1

Reference
Drenan R, Nashmi R, Imoukhuede P, Just H, McKinney S, Lester H. Subcellular trafficking, pentameric assembly, and subunit stoichiometry of neuronal nicotinic acetylcholine receptors containing fluorescently labeled alpha6 and beta3 subunits. Mol Pharmacol. 2008;73:27-41 pubmed

product information

Catalog Number :

50482

Product Name :

pcDNA3.1-nAChr-Alpha6-CFP

article :

doi	10.1124/mol.107.039180
id	7336
pubmed_id	17932221

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5428

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

origin :

pi :

alt_names

Alpha6-CFP

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	KpnI
promoter	CMV
sequencing_primer_3	XFP3 _R CTTGTACAGCTCGTCCATGCC sits on 3 end of the fluorophore and is common to GFP, CFP, YFP and mCherry
sequencing_primer_5	XFP5 _F ATGGTGAGCAAGGGCGAGGAG sits on 5 end of the fluorophore and is common to GFP ,CFP, YFP and mCherry
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Acra6, Nica6
gene	Chrna6
id	11440

genbank_ids

BC031985

mutation

Gly-Ala-Gly flexible linker flanking the CFP open reading frame on both N and C terminal sides

name

nAChr-alpha6

shRNA_sequence

size

3569

species

10090

Mus musculus