This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-nAChr-Alpha6-mCherry
catalog :
50480
citations: 1
Reference
Drenan R, Nashmi R, Imoukhuede P, Just H, McKinney S, Lester H. Subcellular trafficking, pentameric assembly, and subunit stoichiometry of neuronal nicotinic acetylcholine receptors containing fluorescently labeled alpha6 and beta3 subunits. Mol Pharmacol. 2008;73:27-41 pubmed
product information
Catalog Number :
50480
Product Name :
pcDNA3.1-nAChr-Alpha6-mCherry
article :
doi10.1124/mol.107.039180
id7336
pubmed_id17932221
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
origin :
37
pi :
alt_names
Alpha6-mCherry
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5KpnI
promoterCMV
sequencing_primer_3XFP3 _R CTTGTACAGCTCGTCCATGCC sits on 3 end of the fluorophore and is common to GFP, CFP, YFP and mCherry
sequencing_primer_5XFP5 _F ATGGTGAGCAAGGGCGAGGAG sits on 5 end of the fluorophore and is common to GFP ,CFP, YFP and mCherry
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAcra6, Nica6
geneChrna6
id11440
genbank_ids
BC031985
mutation
namenAChr-alpha6
shRNA_sequence
size3569
species
10090
Mus musculus
tags
location
tagmCherry fusion in M3-M4 loop (after residue A405)
resistance markers :
288
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA