This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-CaMKIIa-hM3D(Gq)-mCherry
catalog :
50476
citations: 17
Reference
Lee M, Peng W, Wu C, Kan H, Wang D, Teng Y, et al. Impaired Ventrolateral Periaqueductal Gray-Ventral Tegmental area Pathway Contributes to Chronic Pain-Induced Depression-Like Behavior in Mice. Mol Neurobiol. 2023;: pubmed publisher
Kim Y, Lee G, Shim S, Kim D, Oh S. A Critical Involvement of Glutamatergic Neurons in the Anterior Insular Cortex for Subdiaphragmatic Vagotomy-induced Analgesia. Exp Neurobiol. 2023;32:68-82 pubmed publisher
Inaba H, Li H, Kawatake Kuno A, Dewa K, Nagai J, Oishi N, et al. GPCR-mediated calcium and cAMP signaling determines psychosocial stress susceptibility and resiliency. Sci Adv. 2023;9:eade5397 pubmed publisher
Yen T, Huang T, Lin M, Hsu T, Lu M, Shih P, et al. Sex bias in social deficits, neural circuits and nutrient demand in Cttnbp2 autism models. Brain. 2022;: pubmed publisher
Halbout B, Hutson C, Wassum K, Ostlund S. Dorsomedial prefrontal cortex activation disrupts Pavlovian incentive motivation. Front Behav Neurosci. 2022;16:999320 pubmed publisher
Lamothe Molina P, Franzelin A, Beck L, Li D, Auksutat L, Fieblinger T, et al. ΔFosB accumulation in hippocampal granule cells drives cFos pattern separation during spatial learning. Nat Commun. 2022;13:6376 pubmed publisher
Li M, Zhou H, Teng S, Yang G. Activation of VIP interneurons in the prefrontal cortex ameliorates neuropathic pain aversiveness. Cell Rep. 2022;40:111333 pubmed publisher
Schwitalla J, Pakusch J, Mücher B, Bruckner A, Depke D, Fenzl T, et al. Controlling absence seizures from the cerebellar nuclei via activation of the Gq signaling pathway. Cell Mol Life Sci. 2022;79:197 pubmed publisher
Lee E, Park J, Kwon H, Han P. Repeated exposure with short-term behavioral stress resolves pre-existing stress-induced depressive-like behavior in mice. Nat Commun. 2021;12:6682 pubmed publisher
Silva B, Astori S, Burns A, Heiser H, van den Heuvel L, Santoni G, et al. A thalamo-amygdalar circuit underlying the extinction of remote fear memories. Nat Neurosci. 2021;24:964-974 pubmed publisher
Chao O, Zhang H, Pathak S, Huston J, Yang Y. Functional Convergence of Motor and Social Processes in Lobule IV/V of the Mouse Cerebellum. Cerebellum. 2021;: pubmed publisher
Umpierre A, Bystrom L, Ying Y, Liu Y, Worrell G, Wu L. Microglial calcium signaling is attuned to neuronal activity in awake mice. elife. 2020;9: pubmed publisher
Tomek S, Stegmann G, Leyrer Jackson J, Piña J, Olive M. Restoration of prosocial behavior in rats after heroin self-administration via chemogenetic activation of the anterior insular cortex. Soc Neurosci. 2020;:1-12 pubmed publisher
Chao O, Marron Fernandez de Velasco E, Pathak S, Maitra S, Zhang H, Duvick L, et al. Targeting inhibitory cerebellar circuitry to alleviate behavioral deficits in a mouse model for studying idiopathic autism. Neuropsychopharmacology. 2020;45:1159-1170 pubmed publisher
Miyawaki T, Morikawa S, Susaki E, Nakashima A, Takeuchi H, Yamaguchi S, et al. Visualization and molecular characterization of whole-brain vascular networks with capillary resolution. Nat Commun. 2020;11:1104 pubmed publisher
Siemsen B, McFaddin J, Haigh K, Brock A, Nan Leath M, Hooker K, et al. Amperometric measurements of cocaine cue and novel context-evoked glutamate and nitric oxide release in the nucleus accumbens core. J Neurochem. 2020;153:599-616 pubmed publisher
Chandrasekar A, Heuvel F, Tar L, Hagenston A, Palmer A, Linkus B, et al. Parvalbumin Interneurons Shape Neuronal Vulnerability in Blunt TBI. Cereb Cortex. 2019;29:2701-2715 pubmed publisher
product information
Catalog Number :
50476
Product Name :
pAAV-CaMKIIa-hM3D(Gq)-mCherry
article :
doi
id7641
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutation
backbone_origin
backbone_size4818
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
AAV
growth notes :
These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.
growth strain :
Gq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR I
cloning_site_5Sal I
promoterCaMKIIa
sequencing_primer_3GCATTAAAGCAGCGTATCCACATAGC
sequencing_primer_5ATGCTGACGAAGGCTCGCGA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesEGBRS, HM3, PBS, m3AChR
geneCHRM3
id1131
genbank_ids
mutationSee supplemental documents for DREADD mutations
namehM3D(Gq)-mCherry
shRNA_sequence
size2502
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmCherry
resistance markers :
1493
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA