pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine | Addgene 50463 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

catalog :

50463

citations: 5

Reference
Nahar L, Grant C, Hewett C, Cortes D, Reker A, Kang S, et al. Regulation of Pv-specific interneurons in the medial prefrontal cortex and reward-seeking behaviors. J Neurochem. 2021;156:212-224 pubmed publisher
Denaxa M, Neves G, Burrone J, Pachnis V. Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains. J Vis Exp. 2019;: pubmed publisher
Wang Q, Shen F, Zou R, Zheng J, Yu X, Wang Y. Ketamine-induced apoptosis in the mouse cerebral cortex follows similar characteristic of physiological apoptosis and can be regulated by neuronal activity. Mol Brain. 2017;10:24 pubmed publisher
Li S, Yang C, Zhang L, Gao X, Wang X, Liu W, et al. Promoting axon regeneration in the adult CNS by modulation of the melanopsin/GPCR signaling. Proc Natl Acad Sci U S A. 2016;113:1937-42 pubmed publisher
Yuan P, Grutzendler J. Attenuation of Î²-Amyloid Deposition and Neurotoxicity by Chemogenetic Modulation of Neural Activity. J Neurosci. 2016;36:632-41 pubmed publisher

product information

Catalog Number :

50463

Product Name :

pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

article :

doi
id	7641
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pAAV

backbone_mutation

backbone_origin

backbone_size

4818

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

AAV

growth notes :

These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.

growth strain :

Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoR I
cloning_site_5	Sal I
promoter	human Synapsin 1
sequencing_primer_3	GCATTAAAGCAGCGTATCCACATAGC
sequencing_primer_5	TCGTGTCGTGCCTGAGAGCG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	EGBRS, HM3, PBS, m3AChR
gene	CHRM3
id	1131

genbank_ids

mutation

See supplemental documents for DREADD mutations

name

hM3D(Gq)-IRES-mCitrine

shRNA_sequence

size

3124

species

9606

Homo sapiens