pAAV-EF1a-DIO-hM3D(Gq)-mCherry | Addgene 50460 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAAV-EF1a-DIO-hM3D(Gq)-mCherry

catalog :

50460

citations: 16

Reference
Huang Hobbs E, Cheng Y, Ko Y, Luna Figueroa E, Lozzi B, Taylor K, et al. Remote neuronal activity drives glioma progression through SEMA4F. Nature. 2023;619:844-850 pubmed publisher
Calafate S, xd6 zturan G, Thrupp N, Vanderlinden J, Santa Marinha L, Morais Ribeiro R, et al. Early alterations in the MCH system link aberrant neuronal activity and sleep disturbances in a mouse model of Alzheimer's disease. Nat Neurosci. 2023;26:1021-1031 pubmed publisher
Takahashi T, Hirano A, Kanda T, Saito V, Ashitomi H, Tanaka K, et al. Optogenetic induction of hibernation-like state with modified human Opsin4 in mice. Cell Rep Methods. 2022;2:100336 pubmed publisher
Willmore L, Cameron C, Yang J, Witten I, Falkner A. Behavioural and dopaminergic signatures of resilience. Nature. 2022;611:124-132 pubmed publisher
Herrera Moro Chao D, Kirchner M, Pham C, Foppen E, Denis R, Castel J, et al. Hypothalamic astrocytes control systemic glucose metabolism and energy balance. Cell Metab. 2022;34:1532-1547.e6 pubmed publisher
Sheu S, Upadhyayula S, Dupuy V, Pang S, Deng F, Wan J, et al. A serotonergic axon-cilium synapse drives nuclear signaling to alter chromatin accessibility. Cell. 2022;185:3390-3407.e18 pubmed publisher
Liu S, Ye M, Pao G, Song S, Jhang J, Jiang H, et al. Divergent brainstem opioidergic pathways that coordinate breathing with pain and emotions. Neuron. 2021;: pubmed publisher
Konishi Y, Ichise H, Watabe T, Oki C, Tsukiji S, Hamazaki Y, et al. Intravital Imaging Identifies the VEGF-TXA2 Axis as a Critical Promoter of PGE2 Secretion from Tumor Cells and Immune Evasion. Cancer Res. 2021;81:4124-4132 pubmed publisher
Horio N, Liberles S. Hunger enhances food-odour attraction through a neuropeptide Y spotlight. Nature. 2021;592:262-266 pubmed publisher
Yamada S, Islam M, van Kooten N, Bovee S, Oh Y, Tsujimura A, et al. Neuropeptide Y neurons in the nucleus accumbens modulate anxiety-like behavior. Exp Neurol. 2020;327:113216 pubmed publisher
Aklan I, Sayar Atasoy N, Yavuz Y, Ates T, Coban İ, Koksalar F, et al. NTS Catecholamine Neurons Mediate Hypoglycemic Hunger via Medial Hypothalamic Feeding Pathways. Cell Metab. 2019;: pubmed publisher
Inoue S, Yang R, Tantry A, Davis C, Yang T, Knoedler J, et al. Periodic Remodeling in a Neural Circuit Governs Timing of Female Sexual Behavior. Cell. 2019;179:1393-1408.e16 pubmed publisher
Aggarwal S, Tang C, Sing K, Kim H, Millar R, Tello J. Medial Amygdala Kiss1 Neurons Mediate Female Pheromone Stimulation of Luteinizing Hormone in Male Mice. Neuroendocrinology. 2019;108:172-189 pubmed publisher
Fabelo C, Hernandez J, Chang R, Seng S, Alicea N, Tian S, et al. Endocannabinoid Signaling at Hypothalamic Steroidogenic Factor-1/Proopiomelanocortin Synapses Is Sex- and Diet-Sensitive. Front Mol Neurosci. 2018;11:214 pubmed publisher
Yang T, Yang C, Chizari M, Maheswaranathan N, Burke K, Borius M, et al. Social Control of Hypothalamus-Mediated Male Aggression. Neuron. 2017;95:955-970.e4 pubmed publisher
Conde K, Fabelo C, Krause W, Propst R, Goethel J, Fischer D, et al. Testosterone Rapidly Augments Retrograde Endocannabinoid Signaling in Proopiomelanocortin Neurons to Suppress Glutamatergic Input from Steroidogenic Factor 1 Neurons via Upregulation of Diacylglycerol Lipase-?. Neuroendocrinology. 2017;105:341-356 pubmed publisher

product information

Catalog Number :

50460

Product Name :

pAAV-EF1a-DIO-hM3D(Gq)-mCherry

article :

doi
id	7641
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pAAV

backbone_mutation

backbone_origin

backbone_size

4818

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

AAV

growth notes :

These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.

growth strain :

Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Nhe I
cloning_site_5	Asc I
promoter	EF1a
sequencing_primer_3	GCATTAAAGCAGCGTATCCACATAGC
sequencing_primer_5	TTTTTGAGTTTGGATCTTGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	EGBRS, HM3, PBS, m3AChR
gene	CHRM3
id	1131

genbank_ids

mutation

See supplemental documents for DREADD mutations

name

hM3D(Gq)-mCherry

shRNA_sequence

size

2508

species

9606

Homo sapiens