pAAV-hSyn-DIO-rM3D(Gs)-mCherry | Addgene 50458 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAAV-hSyn-DIO-rM3D(Gs)-mCherry

catalog :

50458

citations: 9

Reference
Priest M, Freda S, Rieth I, Badong D, Dumrongprechachan V, Kozorovitskiy Y. Peptidergic and functional delineation of the Edinger-Westphal nucleus. Cell Rep. 2023;42:112992 pubmed publisher
Licht Murava A, Meadows S, Palaguachi F, Song S, Jackvony S, Bram Y, et al. Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines. Sci Adv. 2023;9:eade1282 pubmed publisher
Inaba H, Li H, Kawatake Kuno A, Dewa K, Nagai J, Oishi N, et al. GPCR-mediated calcium and cAMP signaling determines psychosocial stress susceptibility and resiliency. Sci Adv. 2023;9:eade5397 pubmed publisher
Park G, Fukasawa K, Horie T, Masuo Y, Inaba Y, Tatsuno T, et al. l-Type amino acid transporter 1 in hypothalamic neurons in mice maintains energy and bone homeostasis. JCI Insight. 2023;8: pubmed publisher
Zhang C, Vincelette L, Reimann F, Liberles S. A brainstem circuit for nausea suppression. Cell Rep. 2022;39:110953 pubmed publisher
Zhang T, Perkins M, Chang H, Han W, de Araujo I. An inter-organ neural circuit for appetite suppression. Cell. 2022;185:2478-2494.e28 pubmed publisher
Zhang C, Kaye J, Cai Z, Wang Y, Prescott S, Liberles S. Area Postrema Cell Types that Mediate Nausea-Associated Behaviors. Neuron. 2021;109:461-472.e5 pubmed publisher
Bączyk M, Alami N, Delestrée N, Martinot C, Tang L, Commisso B, et al. Synaptic restoration by cAMP/PKA drives activity-dependent neuroprotection to motoneurons in ALS. J Exp Med. 2020;217: pubmed publisher
Pisansky M, Hanson L, Gottesman I, Gewirtz J. Oxytocin enhances observational fear in mice. Nat Commun. 2017;8:2102 pubmed publisher

product information

Catalog Number :

50458

Product Name :

pAAV-hSyn-DIO-rM3D(Gs)-mCherry

article :

doi
id	7641
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pAAV

backbone_mutation

backbone_origin

backbone_size

4818

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

AAV

growth notes :

These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.

growth strain :

Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	Nhe I
cloning_site_5	Asc I
promoter	human Synapsin 1
sequencing_primer_3	GCATTAAAGCAGCGTATCCACATAGC
sequencing_primer_5	TCGTGTCGTGCCTGAGAGCG
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

See supplemental documents for DREADD mutations

name

rM3D-mCherry

shRNA_sequence

size

1974

species

10116

Rattus norvegicus