pLenti4/TO-hEPO | Addgene 50437 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLenti4/TO-hEPO

catalog :

50437

citations: 2

Reference
Latorre Y, Torres M, Vergara M, Berrios J, Sampayo M, G xf6 decke N, et al. Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO production. Sci Rep. 2023;13:1482 pubmed publisher
Lin R, Dreyzin A, Aamodt K, Li D, Jaminet S, Dudley A, et al. Induction of erythropoiesis using human vascular networks genetically engineered for controlled erythropoietin release. Blood. 2011;118:5420-8 pubmed publisher

product information

Catalog Number :

50437

Product Name :

pLenti4/TO-hEPO

article :

doi	10.1182/blood-2011-08-372946
id	7635
pubmed_id	21937702

bacterial resistance :

Ampicillin

cloning :

backbone

pLenti4/TO/V5-DEST gateway vector

backbone_mutation

backbone_origin

Invitrogen

backbone_size

8598

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

Other

Tetracycline-regulatable (T-REx)

growth notes :

Human erythropoietin (hEPO) ORF with stop codon (582 bp; Ultimate ORF clone number IOH7334) from GeneCopoeia ORF cDNA clones in pDONR vector (GeneCopoeia; GC-A1011) Because the stop codon is inserted before V5 epitope, the V5 tag on the pLenti6.3/V5-DEST vector won t be transcribed.

growth strain :

express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system

origin :

pi :

alt_names

erythropoietin

MVCD2

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV/TO
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	DBAL, ECYT5, EP, MVCD2
gene	EPO
id	2056

genbank_ids

mutation

name

EPO

shRNA_sequence

size

582

species

9606

Homo sapiens