This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti6.3-hEPO
catalog :
50436
citations: 1
Reference
Lin R, Dreyzin A, Aamodt K, Li D, Jaminet S, Dudley A, et al. Induction of erythropoiesis using human vascular networks genetically engineered for controlled erythropoietin release. Blood. 2011;118:5420-8 pubmed publisher
product information
Catalog Number :
50436
Product Name :
pLenti6.3-hEPO
article :
doi10.1182/blood-2011-08-372946
id7635
pubmed_id21937702
bacterial resistance :
Ampicillin
cloning :
backbonepLenti6.3/V5-DEST gateway vector
backbone_mutation
backbone_originInvitrogen
backbone_size9387
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Human erythropoietin (hEPO) ORF with stop codon (582 bp; Ultimate ORF clone number IOH7334) from GeneCopoeia ORF cDNA clones in pDONR vector (GeneCopoeia; GC-A1011). Because the stop codon is inserted before V5 epitope, the V5 tag on the pLenti6.3/V5-DEST vector won t be transcribed.
growth strain :
express human erythropoietin using lentiviral delivery system
origin :
37
pi :
alt_names
erythropoietin
MVCD2
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3WPRE-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDBAL, ECYT5, EP, MVCD2
geneEPO
id2056
genbank_ids
mutation
nameEPO
shRNA_sequence
size582
species
9606
Homo sapiens
tags
resistance markers :
1789
tags :
Unknown
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA