MLM3739 | Addgene 49959 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MLM3739

catalog :

49959

citations: 3

Reference
Liu X, Wu H, Krzisch M, Wu X, Graef J, Muffat J, et al. Rescue of Fragile X Syndrome Neurons by DNA Methylation Editing of the FMR1 Gene. Cell. 2018;172:979-992.e6 pubmed publisher
Liu X, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, et al. Editing DNA Methylation in the Mammalian Genome. Cell. 2016;167:233-247.e17 pubmed publisher
Maeder M, Angstman J, Richardson M, Linder S, Cascio V, Tsai S, et al. Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Nat Biotechnol. 2013;31:1137-42 pubmed publisher

product information

Catalog Number :

49959

Product Name :

MLM3739

article :

doi	10.1038/nbt.2726
id	7448
pubmed_id	24108092

bacterial resistance :

Ampicillin

cloning :

backbone

pJFA345D4

backbone_mutation

backbone_origin

Joung lab (Addgene plasmid #49238)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

TALEN

growth notes :

TALE target site is: TCTTCAATATGCTTACCAAG. Note that due to the TET1 mutations, this plasmid neither demethylated the proximal CpGs nor activated HBB gene expression. See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.

growth strain :

Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control

origin :

pi :

alt_names

HB-4

human beta-globin (HBB) site #4

cloning

clone_method	Restriction Enzyme
cloning_site_3	BsmBI
cloning_site_5	BsmBI
promoter	EF1a
sequencing_primer_3	OJA199 5'-CACCATCACAGCAGTTGG-3'
sequencing_primer_5	EF1a-F 5'-TCAAGCCTCAGACAGTGGTTC-3'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CD113t-C, ECYT6, beta-globin
gene	HBB
id	3043

genbank_ids

mutation

catalytic domain of TET1 inactive (H1671Y and D1673A mutations)

name

3x Flag Tet1CDmut HB-4

shRNA_sequence

size

species

9606

Homo sapiens