MLM3762 | Addgene 49947 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MLM3762

catalog :

49947

citations: 1

Reference
Maeder M, Angstman J, Richardson M, Linder S, Cascio V, Tsai S, et al. Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Nat Biotechnol. 2013;31:1137-42 pubmed publisher

product information

Catalog Number :

49947

Product Name :

MLM3762

article :

doi	10.1038/nbt.2726
id	7448
pubmed_id	24108092

bacterial resistance :

Ampicillin

cloning :

backbone

pJFA334E9

backbone_mutation

backbone_origin

Joung lab (Addgene plasmid #49237)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

TALEN

growth notes :

TALE target site is: TCAGCACAGCAGCTTCCCCC. Note that due to the TET1 mutations, this plasmid neither demethylated the proximal CpGs nor activated RHOXF2 gene expression. See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.

growth strain :

Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene for use as a control

origin :

pi :

alt_names

RH-4

RHOXF2 site #4

cloning

clone_method	Restriction Enzyme
cloning_site_3	BsmBI
cloning_site_5	BsmBI
promoter	EF1a
sequencing_primer_3	OJA199 5'-CACCATCACAGCAGTTGG-3'
sequencing_primer_5	EF1a-F 5'-TCAAGCCTCAGACAGTGGTTC-3'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CT107, PEPP-2, PEPP2, THG1
gene	RHOXF2
id	84528

genbank_ids

mutation

catalytic domain of TET1 inactive (H1671Y and D1673A mutations)

name

3x FLAG Tet1CDmut RH-4

shRNA_sequence

size

species

9606

Homo sapiens