This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MLM3713
catalog :
49946
citations: 2
Reference
Ciccarone F, Valentini E, Zampieri M, Caiafa P. 5mC-hydroxylase activity is influenced by the PARylation of TET1 enzyme. Oncotarget. 2015;6:24333-47 pubmed
Maeder M, Angstman J, Richardson M, Linder S, Cascio V, Tsai S, et al. Targeted DNA demethylation and activation of endogenous genes using programmable TALE-TET1 fusion proteins. Nat Biotechnol. 2013;31:1137-42 pubmed publisher
product information
Catalog Number :
49946
Product Name :
MLM3713
article :
doi10.1038/nbt.2726
id7448
pubmed_id24108092
bacterial resistance :
Ampicillin
cloning :
backbonepJFA334E9
backbone_mutation
backbone_originJoung lab (Addgene plasmid #49237)
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
TALEN
growth notes :
TALE target site is: TCAGCACAGCAGCTTCCCCC. See Supplementary Figure 13 of associated publication for amino acid sequences of backbone and TALE repeat array.
growth strain :
Expresses a 3x Flag TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site
origin :
37
pi :
alt_names
RH-4
RHOXF2 site #4
cloning
clone_methodRestriction Enzyme
cloning_site_3BsmBI
cloning_site_5BsmBI
promoterEF1a
sequencing_primer_3OJA199 5'-CACCATCACAGCAGTTGG-3'
sequencing_primer_5EF1a-F 5'-TCAAGCCTCAGACAGTGGTTC-3'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCT107, PEPP-2, PEPP2, THG1
geneRHOXF2
id84528
genbank_ids
mutation
name3x FLAG Tet1CD RH-4
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3x FLAG
resistance markers :
236
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA