pDEST-mCherry-GJA1-20k-N1 | Addgene 49861 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDEST-mCherry-GJA1-20k-N1

catalog :

49861

citations: 3

Reference
Shimura D, Nuebel E, Baum R, Valdez S, Xiao S, Warren J, et al. Protective mitochondrial fission induced by stress-responsive protein GJA1-20k. elife. 2021;10: pubmed publisher
Nadeem L, Shynlova O, Mesiano S, Lye S. Progesterone Via its Type-A Receptor Promotes Myometrial Gap Junction Coupling. Sci Rep. 2017;7:13357 pubmed publisher
Smyth J, Shaw R. Autoregulation of connexin43 gap junction formation by internally translated isoforms. Cell Rep. 2013;5:611-8 pubmed publisher

product information

Catalog Number :

49861

Product Name :

pDEST-mCherry-GJA1-20k-N1

article :

doi	10.1016/j.celrep.2013.10.009
id	7573
pubmed_id	24210816

bacterial resistance :

Kanamycin

cloning :

backbone

pmcherry-N1

backbone_mutation

Gateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.

backbone_origin

Clontech

backbone_size

4722

promoter

CMV

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

origin :

pi :

alt_names

GJA1

Connexin 43

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	mCherrry
sequencing_primer_5	CMV
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA
gene	GJA1
id	2697

genbank_ids

NM_000165.3

mutation

GJA1-20k corresponds to residues 213 to 382 to Connexin 43. No stop codon to create c-terminal fusion

name

GJA1-20k

shRNA_sequence

size

510

species

9606

Homo sapiens