pDEST-EGFP-Cx43ML-N1 | Addgene 49860 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDEST-EGFP-Cx43ML-N1

catalog :

49860

citations: 3

Reference
Tishchenko A, Azorín D, Vidal Brime L, Muñoz M, Arenas P, Pearce C, et al. Cx43 and Associated Cell Signaling Pathways Regulate Tunneling Nanotubes in Breast Cancer Cells. Cancers (Basel). 2020;12: pubmed publisher
Nadeem L, Shynlova O, Mesiano S, Lye S. Progesterone Via its Type-A Receptor Promotes Myometrial Gap Junction Coupling. Sci Rep. 2017;7:13357 pubmed publisher
Smyth J, Shaw R. Autoregulation of connexin43 gap junction formation by internally translated isoforms. Cell Rep. 2013;5:611-8 pubmed publisher

product information

Catalog Number :

49860

Product Name :

pDEST-EGFP-Cx43ML-N1

article :

doi	10.1016/j.celrep.2013.10.009
id	7573
pubmed_id	24210816

bacterial resistance :

Kanamycin

cloning :

backbone

pegfp-N1

backbone_mutation

Gateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.

backbone_origin

Clontech

backbone_size

4722

promoter

CMV

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal EGFP fusion tag

origin :

pi :

alt_names

GJA1

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	EGFP
sequencing_primer_5	CMV
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA
gene	GJA1
id	2697

genbank_ids

NM_000165.3

mutation

All internal Methionines mutated to Leucine. No stop codon to create c-terminal fusion

name

Connexin 43

shRNA_sequence

size

1146

species

9606

Homo sapiens