pcDNA3.2-GJA1-20K-V5 | Addgene 49859 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.2-GJA1-20K-V5

catalog :

49859

citations: 1

Reference
Smyth J, Shaw R. Autoregulation of connexin43 gap junction formation by internally translated isoforms. Cell Rep. 2013;5:611-8 pubmed publisher

product information

Catalog Number :

49859

Product Name :

pcDNA3.2-GJA1-20K-V5

article :

doi	10.1016/j.celrep.2013.10.009
id	7573
pubmed_id	24210816

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.2/V5-DEST

backbone_mutation

backbone_origin

Life Technologies

backbone_size

7711

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Encodes C-terminal fragment of human connexin 43 initiating at M213, with M281 and M320 mutated to L, and a V5 C-terminal fusion tag

origin :

pi :

alt_names

GJA1

Connexin 43

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	V5
sequencing_primer_5	CMV
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA
gene	GJA1
id	2697

genbank_ids

NM_000165.3

mutation

GJA1-20k fragment corresponds to residues 213-382 of Connexin 43, no stop codon to allow for c-terminal fusion

name

GJA1-20k

shRNA_sequence

size

510

species

9606

Homo sapiens