pcDNA3.2-Cx43-ML-HA | Addgene 49857 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.2-Cx43-ML-HA

catalog :

49857

citations: 1

Reference
Smyth J, Shaw R. Autoregulation of connexin43 gap junction formation by internally translated isoforms. Cell Rep. 2013;5:611-8 pubmed publisher

product information

Catalog Number :

49857

Product Name :

pcDNA3.2-Cx43-ML-HA

article :

doi	10.1016/j.celrep.2013.10.009
id	7573
pubmed_id	24210816

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.2/HA-DEST

backbone_mutation

Replaced V5 c-terminal fusion tag with HA tag

backbone_origin

Invitrogen

backbone_size

7711

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag

origin :

pi :

alt_names

GJA1

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	TK-pA-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA
gene	GJA1
id	2697

genbank_ids

NM_000165.3

mutation

All internal Methionines mutated to Leucine. Contains several wobble mutations to confer resistance to siRNA. No stop codon to create c-terminal fusion

name

Connexin 43

shRNA_sequence

size

1146

species

9606

Homo sapiens