This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.2-Cx43-M100/125/147L-siResist
catalog :
49856
citations: 1
Reference
Smyth J, Shaw R. Autoregulation of connexin43 gap junction formation by internally translated isoforms. Cell Rep. 2013;5:611-8 pubmed publisher
product information
Catalog Number :
49856
Product Name :
pcDNA3.2-Cx43-M100/125/147L-siResist
article :
doi10.1016/j.celrep.2013.10.009
id7573
pubmed_id24210816
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.2/V5-DEST
backbone_mutation
backbone_originLife Technologies
backbone_size7711
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Encodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNA
origin :
37
pi :
alt_names
GJA1
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3V5
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA
geneGJA1
id2697
genbank_ids
NM_000165.3
mutationMethionine100, Methionine 125, and Methionine 147 mutated to Leucine. Contains several wobble mutations to confer resistance to siRNA
nameConnexin 43
shRNA_sequence
size1146
species
9606
Homo sapiens
tags
resistance markers :
877
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA