This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pICH47742::2x35S-5 UTR-hCas9(STOP)-NOST
catalog :
49771
citations: 17
Reference
Giacomelli L, Zeilmaker T, Giovannini O, Salvagnin U, Masuero D, Franceschi P, et al. Simultaneous editing of two DMR6 genes in grapevine results in reduced susceptibility to downy mildew. Front Plant Sci. 2023;14:1242240 pubmed publisher
Procko C, Wong W, Patel J, Mousavi S, Dabi T, Duque M, et al. Mutational analysis of mechanosensitive ion channels in the carnivorous Venus flytrap plant. Curr Biol. 2023;: pubmed publisher
Drapal M, Enfissi E, Almeida J, Rapacz E, Nogueira M, Fraser P. The potential of metabolomics in assessing global compositional changes resulting from the application of CRISPR/Cas9 technologies. Transgenic Res. 2023;: pubmed publisher
Shelake R, Pramanik D, Kim J. Improved Dual Base Editor Systems (iACBEs) for Simultaneous Conversion of Adenine and Cytosine in the Bacterium Escherichia coli. MBio. 2023;:e0229622 pubmed publisher
Son G, Moon J, Shelake R, Vuong U, Ingle R, Gassmann W, et al. Conserved Opposite Functions in Plant Resistance to Biotrophic and Necrotrophic Pathogens of the Immune Regulator SRFR1. Int J Mol Sci. 2021;22: pubmed publisher
Pramanik D, Shelake R, Park J, Kim M, Hwang I, Park Y, et al. CRISPR/Cas9-Mediated Generation of Pathogen-Resistant Tomato against Tomato Yellow Leaf Curl Virus and Powdery Mildew. Int J Mol Sci. 2021;22: pubmed publisher
Tran M, Doan D, Kim J, Song Y, Sung Y, Das S, et al. CRISPR/Cas9-based precise excision of SlHyPRP1 domain(s) to obtain salt stress-tolerant tomato. Plant Cell Rep. 2020;: pubmed publisher
Kourelis J, Malik S, Mattinson O, Krauter S, Kahlon P, Paulus J, et al. Evolution of a guarded decoy protease and its receptor in solanaceous plants. Nat Commun. 2020;11:4393 pubmed publisher
Fan P, Wang P, Lou Y, Leong B, Moore B, Schenck C, et al. Evolution of a plant gene cluster in Solanaceae and emergence of metabolic diversity. elife. 2020;9: pubmed publisher
Israeli A, Capua Y, Shwartz I, Tal L, Meir Z, Levy M, et al. Multiple Auxin-Response Regulators Enable Stability and Variability in Leaf Development. Curr Biol. 2019;: pubmed publisher
Wu S, Zhang B, Keyhaninejad N, Rodríguez G, Kim H, Chakrabarti M, et al. A common genetic mechanism underlies morphological diversity in fruits and other plant organs. Nat Commun. 2018;9:4734 pubmed publisher
Tomlinson L, Yang Y, Emenecker R, Smoker M, Taylor J, Perkins S, et al. Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele. Plant Biotechnol J. 2019;17:132-140 pubmed publisher
Bollier N, Sicard A, Leblond J, Latrasse D, Gonzalez N, Gévaudant F, et al. At-MINI ZINC FINGER2 and Sl-INHIBITOR OF MERISTEM ACTIVITY, a Conserved Missing Link in the Regulation of Floral Meristem Termination in Arabidopsis and Tomato. Plant Cell. 2018;30:83-100 pubmed publisher
Rodríguez Leal D, Lemmon Z, Man J, Bartlett M, Lippman Z. Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing. Cell. 2017;171:470-480.e8 pubmed publisher
Alexandrov A, Shu M, Steitz J. Fluorescence Amplification Method for Forward Genetic Discovery of Factors in Human mRNA Degradation. Mol Cell. 2017;65:191-201 pubmed publisher
Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, et al. Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2017;89:155-168 pubmed publisher
Belhaj K, Chaparro Garcia A, Kamoun S, Nekrasov V. Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013;9:39 pubmed publisher
product information
Catalog Number :
49771
Product Name :
pICH47742::2x35S-5 UTR-hCas9(STOP)-NOST
article :
doi10.1186/1746-4811-9-39
id7552
pubmed_id24112467
bacterial resistance :
Ampicillin
cloning :
backbonepICH47742
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth notes :
We removed BsaI and BbsI sites from hCas9 preserving the amino acid composition and placed it under the 35S promoter using the Golden Gate system from S. Marillonnet.
growth strain :
Level 1 hCas9 module
origin :
37
pi :
alt_names
cloning
entrez_gene
genbank_ids
mutation
name35Sp::hCas9
shRNA_sequence
size
species
100
Synthetic
tags
plasmid copy :
hCas9 from George Church
resistance markers :
1659
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA