This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pICH41308::hCas9
catalog :
49770
citations: 5
Reference
Cheng Y, Chen G, Yang X, Wang Y, Hong L. Effects of HPV Pseudotype Virus in Cutting E6 Gene Selectively in SiHa Cells. Curr Med Sci. 2018;38:212-221 pubmed publisher
Alexandrov A, Shu M, Steitz J. Fluorescence Amplification Method for Forward Genetic Discovery of Factors in Human mRNA Degradation. Mol Cell. 2017;65:191-201 pubmed publisher
Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, et al. Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit. Plant J. 2017;89:155-168 pubmed publisher
Pohl C, Kiel J, Driessen A, Bovenberg R, Nygård Y. CRISPR/Cas9 Based Genome Editing of Penicillium chrysogenum. ACS Synth Biol. 2016;5:754-64 pubmed publisher
Belhaj K, Chaparro Garcia A, Kamoun S, Nekrasov V. Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods. 2013;9:39 pubmed publisher
product information
Catalog Number :
49770
Product Name :
pICH41308::hCas9
article :
doi10.1186/1746-4811-9-39
id7552
pubmed_id24112467
bacterial resistance :
Spectinomycin
cloning :
backbonepICH41308
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth notes :
BsaI and BbsI sites have been removed from the sequence by site-directed mutagenesis, however the amino acid sequence is as in the original hCas9.
growth strain :
Level 0 hCas9 module
origin :
37
pi :
alt_names
cloning
entrez_gene
genbank_ids
mutation
namehCas9
shRNA_sequence
size
species
100
Synthetic
tags
plasmid copy :
hCas9 is from George Church.
resistance markers :
1659
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA