pSK+ chickBMPR-1B DN | Addgene 49529 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSK+ chickBMPR-1B DN

catalog :

49529

citations: 1

Reference
Zou H, Wieser R, Massague J, Niswander L. Distinct roles of type I bone morphogenetic protein receptors in the formation and differentiation of cartilage. Genes Dev. 1997;11:2191-203 pubmed

product information

Catalog Number :

49529

Product Name :

pSK+ chickBMPR-1B DN

article :

doi	10.1101/gad.11.17.2191
id	7524
pubmed_id	9303535

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript SK+

backbone_mutation

backbone_origin

Stratagene

backbone_size

2959

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

The BMPR-1B insert in this plasmid contains P2A, M11V and S18G mutations when compared to GenBank reference sequence NP_990463.1. These mutations are not a concern for the function of the plasmid, as described in the associated publication.

growth strain :

cloning vector for dominant negative chick BMP receptor 1B

origin :

pi :

alt_names

rpk-1

BMPR-1B

alk-6

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter	T3
sequencing_primer_3	T7
sequencing_primer_5	T3
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	RPK-1
gene	BMPR1B
id	396030

genbank_ids

mutation

dominant negative mutation (K231R); also P2A, M11V and S18G

name

BMPR-1B DN

shRNA_sequence

size

species

9031

Gallus gallus