This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFP-BICD1
catalog :
49487
citations: 1
Reference
Moorhead A, Rzomp K, Scidmore M. The Rab6 effector Bicaudal D1 associates with Chlamydia trachomatis inclusions in a biovar-specific manner. Infect Immun. 2007;75:781-91 pubmed
product information
Catalog Number :
49487
Product Name :
EGFP-BICD1
article :
doi10.1128/IAI.01447-06
id7518
pubmed_id17101644
bacterial resistance :
Kanamycin
cloning :
backboneEGFP C2
backbone_mutation
backbone_originClontech
backbone_size4371
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
PCR from HeLa cDNA with 5' BamHI Linkers plus 2 additional AA prior to ATG) and 3' SalI linkers cloned into BglII/SalI sites of EGFP C2 **From the associated publication: Upon DNA sequencing, it was determined that amino acids 821 to 920 were deleted from . EGFP-BICD1.
growth strain :
expresses EGFP-BICD1 in mammalian cells
origin :
37
pi :
alt_names
bicaudal D1
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI/BglII
promoterCMV
sequencing_primer_3
sequencing_primer_5EGFP C (CATGGTCCTGCTGGAGTTCGTG)
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesBICD, bic-D 1
geneBICD1
id636
genbank_ids
NM_001003398.1
mutation** see notes
nameBICD1
shRNA_sequence
size2937
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
1762
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA