This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-ATG8(416)/GFP-AUT7(416)
catalog :
49425
citations: 7
Reference
Vijayraghavan S, Kozmin S, Xi W, McCusker J. A novel narnavirus is widespread in Saccharomyces cerevisiae and impacts multiple host phenotypes. G3 (Bethesda). 2022;: pubmed publisher
Brown J, Alibhai J, Zhu E, Frankel A. Methylarginine efflux in nutrient-deprived yeast mitigates disruption of nitric oxide synthesis. Amino Acids. 2022;: pubmed publisher
Sakamaki J, Ode K, Kurikawa Y, Ueda H, Yamamoto H, Mizushima N. Ubiquitination of phosphatidylethanolamine in organellar membranes. Mol Cell. 2022;82:3677-3692.e11 pubmed publisher
Chen X, Muñoz Arellano A, Petranovic D. UBB+1 reduces amyloid-β cytotoxicity by activation of autophagy in yeast. Aging (Albany NY). 2021;13:23953-23980 pubmed publisher
Kainz K, Pendl T, Madeo F, Carmona Gutierrez D. Assessing autophagic flux in yeast. Methods Cell Biol. 2021;164:73-94 pubmed publisher
Plummer J, Johnson J. Extension of Cellular Lifespan by Methionine Restriction Involves Alterations in Central Carbon Metabolism and Is Mitophagy-Dependent. Front Cell Dev Biol. 2019;7:301 pubmed publisher
Guan J, Stromhaug P, George M, Habibzadegah Tari P, Bevan A, Dunn W, et al. Cvt18/Gsa12 is required for cytoplasm-to-vacuole transport, pexophagy, and autophagy in Saccharomyces cerevisiae and Pichia pastoris. Mol Biol Cell. 2001;12:3821-38 pubmed
product information
Catalog Number :
49425
Product Name :
GFP-ATG8(416)/GFP-AUT7(416)
article :
doi10.1091/mbc.12.12.3821
id7496
pubmed_id11739783
bacterial resistance :
Ampicillin
cloning :
backbonepRS416
backbone_mutation
backbone_origin
backbone_size4898
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Yeast Expression
growth strain :
Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.
origin :
37
pi :
alt_names
ATG8
AUT7
APG8
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoterATG8
sequencing_primer_3NA
sequencing_primer_5NA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYBL078C, APG8, AUT7, CVT5
geneATG8
id852200
genbank_ids
mutation
nameautophagy-related 8
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagGFP
resistance markers :
1772
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA