This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
GFP-ATG8(414)/GFP-AUT7(414)
catalog :
49424
citations: 2
Reference
Reinhart E, Litt N, Katzenell S, Pellegrini M, Yamamoto A, Ragusa M. A highly conserved glutamic acid in ALFY inhibits membrane binding to aid in aggregate clearance. Traffic. 2020;: pubmed publisher
Abeliovich H, Zhang C, Dunn W, Shokat K, Klionsky D. Chemical genetic analysis of Apg1 reveals a non-kinase role in the induction of autophagy. Mol Biol Cell. 2003;14:477-90 pubmed
product information
Catalog Number :
49424
Product Name :
GFP-ATG8(414)/GFP-AUT7(414)
article :
doi10.1091/mbc.e02-07-0413
id7495
pubmed_id12589048
bacterial resistance :
Ampicillin
cloning :
backbonepRS414
backbone_mutation
backbone_origin
backbone_size4788
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Yeast Expression
growth strain :
Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.
origin :
37
pi :
alt_names
ATG8
AUT7
APG8
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoterATG8
sequencing_primer_3NA
sequencing_primer_5NA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYBL078C, APG8, AUT7, CVT5
geneATG8
id852200
genbank_ids
mutation
nameautophagy-related 8
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagGFP
resistance markers :
1772
tags :
Low Copy
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA