This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
CuGFP-ATG8(416)
catalog :
49423
citations: 3
Reference
Park S, Park S, Liebman S. TDP-43 Toxicity in Yeast Is Associated with a Reduction in Autophagy, and Deletions of TIP41 and PBP1 Counteract These Effects. Viruses. 2022;14: pubmed publisher
Kainz K, Pendl T, Madeo F, Carmona Gutierrez D. Assessing autophagic flux in yeast. Methods Cell Biol. 2021;164:73-94 pubmed publisher
Wang K, Yang Z, Liu X, Mao K, Nair U, Klionsky D. Phosphatidylinositol 4-kinases are required for autophagic membrane trafficking. J Biol Chem. 2012;287:37964-72 pubmed publisher
product information
Catalog Number :
49423
Product Name :
CuGFP-ATG8(416)
article :
doi10.1074/jbc.M112.371591
id7494
pubmed_id22977244
bacterial resistance :
Ampicillin
cloning :
backbonepRS416
backbone_mutation
backbone_origin
backbone_size4898
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Yeast Expression
growth strain :
Expresses ATG8 fused at the N terminus to GFP under the control of the CUP1 promoter.
origin :
37
pi :
alt_names
ATG8
AUT7
APG8
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XmaI
promoterCUP1
sequencing_primer_3NA
sequencing_primer_5NA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYBL078C, APG8, AUT7, CVT5
geneATG8
id852200
genbank_ids
mutation
nameautophagy-related 8
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tagGFP
resistance markers :
1772
tags :
Low Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA