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product summary
company name :
Addgene
product type :
cDNA
product name :
pLPS-hSox2-gfp
catalog :
49390
product information
Catalog Number :
49390
Product Name :
pLPS-hSox2-gfp
article :
| doi | |
| id | 7484 |
| pubmed_id |
bacterial resistance :
Chloramphenicol and Kanamycin
cloning :
| backbone | pLPS-3'EGFP | ||||
| backbone_mutation | |||||
| backbone_origin | Clontech | ||||
| backbone_size | 4900 | ||||
| promoter | |||||
| sequencing_primer_3 | |||||
| sequencing_primer_5 | |||||
| vector_types |
|
growth notes :
hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites. When the recombinant construct is expressed in mammalian cells, EGFP is expressed from the immediate early promoter of cytomegalovirus (pCMV IE) as a 3' fusion to Sox2, through intron splicing.
growth strain :
mammalian expression of human Sox2 fused to EGFP
origin :
37
pi :
|
resistance markers :
| 1760 |
tags :
High Copy
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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