This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLPS-hAkt1-gfp
catalog :
49388
product information
Catalog Number :
49388
Product Name :
pLPS-hAkt1-gfp
article :
doi
id7484
pubmed_id
bacterial resistance :
Chloramphenicol and Kanamycin
cloning :
backbonepLPS-3'EGFP
backbone_mutation
backbone_originClontech
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Cre/Lox
Other
acceptor vector
growth notes :
hAkt1 was transferred from the donor vector (pDNR-hAkt1, Addgene plasmid #49387) to the acceptor vector using Cre-mediated recombination at the LoxP sites. When the recombinant construct is expressed in mammalian cells, EGFP is expressed from the immediate early promoter of cytomegalovirus (pCMV IE) as a 3' fusion to Akt1, through intron splicing.
growth strain :
mammalian expression of human Akt1 fused to EGFP
origin :
37
pi :
alt_names
v-akt murine thymoma viral oncogene homolog 1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
geneAKT1
id207
genbank_ids
BC000479
mutationtga to cca at nt 1725 with respect to GenBank ID BC000479
nameAkt1
shRNA_sequence
size1487
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
resistance markers :
1760
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA