This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pmirGLO-Bmpr2- 17
catalog :
49380
citations: 2
Reference
Kwak J, Kim M, Kim K. Generation of microRNA-30e-producing recombinant viral hemorrhagic septicemia virus (VHSV) and its effect on in vitro immune responses. Fish Shellfish Immunol. 2019;94:381-388 pubmed publisher
Murphy B, Obad S, Bihannic L, Ayrault O, Zindy F, Kauppinen S, et al. Silencing of the miR-17~92 cluster family inhibits medulloblastoma progression. Cancer Res. 2013;73:7068-78 pubmed publisher
product information
Catalog Number :
49380
Product Name :
pmirGLO-Bmpr2- 17
article :
doi10.1158/0008-5472.CAN-13-0927
id7481
pubmed_id24145352
bacterial resistance :
Ampicillin
cloning :
backbonepmirGLO
backbone_mutationFor cloning oligonucleotides into pmirGLO, bp 7314-7331 were excised (region between PmeI and XbaI).
backbone_originPromega
backbone_size7350
promoterHuman phosphoglycerate kinase (PGK) promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Luciferase
growth strain :
Insert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5PmeI
promoter
sequencing_primer_3CACTGCATTCTAGTTGTGGT
sequencing_primer_5GAAGCTGAGTTGGCTGCT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBMPR-II, BMPR3, BMR2, BRK-3, POVD1, PPH1, T-ALK
geneBMPR2
id659
genbank_ids
NM_001204
mutationchanged nucleotides AC to TG at bp 7347-7348
nameBmpr2
shRNA_sequence
size45
species
9606
Homo sapiens
10090
Mus musculus
tags
resistance markers :
1113
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA