This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAc-y1sgRNA-Cas9
catalog :
49331
citations: 2
Reference
de Toeuf B, Soin R, Nazih A, Dragojevic M, Jurenas D, Delacourt N, et al. ARE-mediated decay controls gene expression and cellular metabolism upon oxygen variations. Sci Rep. 2018;8:5211 pubmed publisher
Bassett A, Tibbit C, Ponting C, Liu J. Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Biol Open. 2014;3:42-9 pubmed publisher
product information
Catalog Number :
49331
Product Name :
pAc-y1sgRNA-Cas9
article :
doi10.1242/bio.20137120
id7696
pubmed_id24326186
bacterial resistance :
Ampicillin
cloning :
backbonepAc-STABLE1-Puro
backbone_mutation
backbone_originDr. James Sutherland
backbone_size6600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
CRISPR
growth notes :
gRNA target sequence GGTTTTGGACACTGGAACCG
growth strain :
Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells
origin :
37
pi :
alt_names
CRISPR associated 9
hspCas9
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5EcoRI
promoterActin-5c
sequencing_primer_3tcgagacaaacggcgaaacc
sequencing_primer_5gagttcttgtgctgtgtgga
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationHuman codon optimised
nameCas9
shRNA_sequence
size4278
species
100
Synthetic
32644
Other
Streptococcus pyogenes
tags
locationN terminal on insert
tag3xFLAG
locationN terminal on insert
tagNLS
locationC terminal on insert
tagNLS
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5BglII
promoterDrosophila U6
sequencing_primer_3AAAAAAGCACCGACTCGGTGC
sequencing_primer_5GTTCGACTTGCAGCCTGAAATACG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namedU6-sgRNA
shRNA_sequence
size500
species
7227
Drosophila melanogaster
100
Synthetic
tags
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BspQI
cloning_site_5BspQI
promoterDrosophila U6
sequencing_primer_3
sequencing_primer_5GTTCGACTTGCAGCCTGAAATACG
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesDmel_CG3757, CG3757, Dmel CG3757, EG:125H10.2, T6, Y
geney
id30980
genbank_ids
mutation
namey1 sgRNA
shRNA_sequence
size20
species
7227
Drosophila melanogaster
tags
plasmid copy :
The human codon optimised spCas9 gene was amplified from pX330 (Addgene plasmid 42230) deposited by Feng Zhang. The pAc-STABLE1-Puro plasmid was obtained from Dr James Sutherland.
resistance markers :
1752
tags :
Low Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA