This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAc-sgRNA-Cas9
catalog :
49330
citations: 33
Reference
Sheng P, Li L, Li T, Wang Y, Hiers N, Mejia J, et al. Screening of Drosophila microRNA-degradation sequences reveals Argonaute1 mRNA's role in regulating miR-999. Nat Commun. 2023;14:2108 pubmed publisher
Walsh E, Torres T, R xfc ckert C. Culex Mosquito Piwi4 Is Antiviral against Two Negative-Sense RNA Viruses. Viruses. 2022;14: pubmed publisher
Klaus L, de Almeida B, Vlasova A, Nem x10d ko F, Schleiffer A, Bergauer K, et al. Systematic identification and characterization of repressive domains in Drosophila transcription factors. EMBO J. 2022;:e112100 pubmed publisher
Kingston E, Blodgett L, Bartel D. Endogenous transcripts direct microRNA degradation in Drosophila, and this targeted degradation is required for proper embryonic development. Mol Cell. 2022;82:3872-3884.e9 pubmed publisher
Torres T, Prince B, Robison A, R xfc ckert C. Optimized In Vitro CRISPR/Cas9 Gene Editing Tool in the West Nile Virus Mosquito Vector, Culex quinquefasciatus. Insects. 2022;13: pubmed publisher
Hendy O, Serebreni L, Bergauer K, Muerdter F, Huber L, Nem x10d ko F, et al. Developmental and housekeeping transcriptional programs in Drosophila require distinct chromatin remodelers. Mol Cell. 2022;82:3598-3612.e7 pubmed publisher
Meng Q, Stoyko D, Andrews C, Konstantinidou P, Genzor P, O T, et al. Functional editing of endogenous genes through rapid selection of cell pools (Rapid generation of endogenously tagged genes in Drosophila ovarian somatic sheath cells). Nucleic Acids Res. 2022;: pubmed publisher
de Malmanche H, Marcellin E, Reid S. Knockout of Sf-Caspase-1 generates apoptosis-resistant Sf9 cell lines: Implications for baculovirus expression. Biotechnol J. 2022;:e2100532 pubmed publisher
Emtenani S, Martin E, Gyoergy A, Bicher J, Genger J, Kocher T, et al. Macrophage mitochondrial bioenergetics and tissue invasion are boosted by an Atossa-Porthos axis in Drosophila. EMBO J. 2022;41:e109049 pubmed publisher
Andreev V, Yu C, Wang J, Schnabl J, Tirián L, Gehre M, et al. Panoramix SUMOylation on chromatin connects the piRNA pathway to the cellular heterochromatin machinery. Nat Struct Mol Biol. 2022;29:130-142 pubmed publisher
Genzor P, Konstantinidou P, Stoyko D, Manzourolajdad A, Marlin Andrews C, Elchert A, et al. Cellular abundance shapes function in piRNA-guided genome defense. Genome Res. 2021;31:2058-2068 pubmed publisher
Scherer C, Knowles J, Sreenu V, Fredericks A, Fuss J, Maringer K, et al. An Aedes aegypti-Derived Ago2 Knockout Cell Line to Investigate Arbovirus Infections. Viruses. 2021;13: pubmed publisher
Kingston E, Bartel D. Ago2 protects Drosophila siRNAs and microRNAs from target-directed degradation, even in the absence of 2'-O-methylation. RNA. 2021;27:710-724 pubmed publisher
Schnabl J, Wang J, Hohmann U, Gehre M, Batki J, Andreev V, et al. Molecular principles of Piwi-mediated cotranscriptional silencing through the dimeric SFiNX complex. Genes Dev. 2021;: pubmed publisher
Azlan A, Obeidat S, Theva Das K, Yunus M, Azzam G. Genome-wide identification of Aedes albopictus long noncoding RNAs and their association with dengue and Zika virus infection. PLoS Negl Trop Dis. 2021;15:e0008351 pubmed publisher
Bobkov G, Huang A, van den Berg S, Mitra S, Anselm E, Lazou V, et al. Spt6 is a maintenance factor for centromeric CENP-A. Nat Commun. 2020;11:2919 pubmed publisher
Kulkarni A, Yu W, Moon A, Pandey A, Hanley K, Xu J. Programmable CRISPR interference for gene silencing using Cas13a in mosquitoes. J Genomics. 2020;8:30-36 pubmed publisher
Batki J, Schnabl J, Wang J, Handler D, Andreev V, Stieger C, et al. The nascent RNA binding complex SFiNX licenses piRNA-guided heterochromatin formation. Nat Struct Mol Biol. 2019;26:720-731 pubmed publisher
Reichholf B, Herzog V, Fasching N, Manzenreither R, Sowemimo I, Ameres S. Time-Resolved Small RNA Sequencing Unravels the Molecular Principles of MicroRNA Homeostasis. Mol Cell. 2019;75:756-768.e7 pubmed publisher
Pandey A, Li Kroeger D, Sethi M, Lee T, Buettner F, Bakker H, et al. Sensitized genetic backgrounds reveal differential roles for EGF repeat xylosyltransferases in Drosophila Notch signaling. Glycobiology. 2018;28:849-859 pubmed publisher
Lloyd D, Toegel M, Fulga T, Wilkie A. The Drosophila homologue of MEGF8 is essential for early development. Sci Rep. 2018;8:8790 pubmed publisher
de Toeuf B, Soin R, Nazih A, Dragojevic M, Jurenas D, Delacourt N, et al. ARE-mediated decay controls gene expression and cellular metabolism upon oxygen variations. Sci Rep. 2018;8:5211 pubmed publisher
Wu Q, Ferry Q, Baeumler T, Michaels Y, Vitsios D, Habib O, et al. In situ functional dissection of RNA cis-regulatory elements by multiplex CRISPR-Cas9 genome engineering. Nat Commun. 2017;8:2109 pubmed publisher
Ghosh S, Liu J. Genomic Tagging of AGO1 Using CRISPR/Cas9-Mediated Homologous Recombination. Methods Mol Biol. 2018;1680:217-235 pubmed publisher
Franz A, Brunner E, Basler K. Generation of genome-modified Drosophila cell lines using SwAP. Fly (Austin). 2017;11:303-311 pubmed publisher
Varjak M, Maringer K, Watson M, Sreenu V, Fredericks A, Pondeville E, et al. Aedes aegypti Piwi4 Is a Noncanonical PIWI Protein Involved in Antiviral Responses. mSphere. 2017;2: pubmed publisher
Franz A, Shlyueva D, Brunner E, Stark A, Basler K. Probing the canonicity of the Wnt/Wingless signaling pathway. PLoS Genet. 2017;13:e1006700 pubmed publisher
Ali T, Kruger M, Bhuju S, Jarek M, Bartkuhn M, Renkawitz R. Chromatin binding of Gcn5 in Drosophila is largely mediated by CP190. Nucleic Acids Res. 2017;45:2384-2395 pubmed publisher
Ahmad M, Xue Y, Lee S, Martindale J, Shen W, Li W, et al. RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals. Nucleic Acids Res. 2016;44:6335-49 pubmed publisher
Mabashi Asazuma H, Kuo C, Khoo K, Jarvis D. Modifying an Insect Cell N-Glycan Processing Pathway Using CRISPR-Cas Technology. ACS Chem Biol. 2015;10:2199-208 pubmed publisher
Bassett A, Kong L, Liu J. A genome-wide CRISPR library for high-throughput genetic screening in Drosophila cells. J Genet Genomics. 2015;42:301-9 pubmed publisher
Bassett A, Azzam G, Wheatley L, Tibbit C, Rajakumar T, McGowan S, et al. Understanding functional miRNA-target interactions in vivo by site-specific genome engineering. Nat Commun. 2014;5:4640 pubmed publisher
Bassett A, Tibbit C, Ponting C, Liu J. Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9. Biol Open. 2014;3:42-9 pubmed publisher
product information
Catalog Number :
49330
Product Name :
pAc-sgRNA-Cas9
article :
doi10.1242/bio.20137120
id7696
pubmed_id24326186
bacterial resistance :
Ampicillin
cloning :
backbonepAc-STABLE1-Puro
backbone_mutation
backbone_originDr. James Sutherland
backbone_size6600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
CRISPR
growth notes :
There are mismatches in actin promoter region. Plasmid should be fine.
growth strain :
Expresses sgRNA and Cas9-Puro in Drosophila S2 cells
origin :
37
pi :
alt_names
CRISPR associated 9
hspCas9
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5EcoRI
promoterActin-5c
sequencing_primer_3tcgagacaaacggcgaaacc
sequencing_primer_5gagttcttgtgctgtgtgga
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationHuman codon optimised
nameCas9
shRNA_sequence
size4278
species
100
Synthetic
32644
Other
Streptococcus pyogenes
tags
locationN terminal on insert
tag3xFLAG
locationN terminal on insert
tagNLS
locationC terminal on insert
tagNLS
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BglII
cloning_site_5BglII
promoterDrosophila U6
sequencing_primer_3GTTCGACTTGCAGCCTGAAATACG
sequencing_primer_5AAAAAAGCACCGACTCGGTGC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namedU6-sgRNA
shRNA_sequence
size500
species
7227
Drosophila melanogaster
100
Synthetic
tags
plasmid copy :
The human codon optimised spCas9 gene was amplified from pX330 (Addgene plasmid 42230) deposited by Feng Zhang. The pAc-STABLE1-Puro plasmid was obtained from Dr James Sutherland.
resistance markers :
1752
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA