This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PSL1180polyUBdsRED
catalog :
49327
citations: 3
Reference
Raji J, Melo N, Castillo J, González S, Saldana V, Stensmyr M, et al. Aedes aegypti Mosquitoes Detect Acidic Volatiles Found in Human Odor Using the IR8a Pathway. Curr Biol. 2019;29:1253-1262.e7 pubmed publisher
Corfas R, Vosshall L. The cation channel TRPA1 tunes mosquito thermotaxis to host temperatures. elife. 2015;4: pubmed publisher
Kistler K, Vosshall L, Matthews B. Genome engineering with CRISPR-Cas9 in the mosquito Aedes aegypti. Cell Rep. 2015;11:51-60 pubmed publisher
product information
Catalog Number :
49327
Product Name :
PSL1180polyUBdsRED
article :
doi
id7468
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepsl1180
backbone_mutationAedes polyubiquitin promoter and dsRED were cloned as a single fragment into psl1180 using BamH1 and Mlu1.
backbone_origin
backbone_size3422
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
Addgene's quality control sequencing finds a one nucleotide discrepancy in the Aedes aegypti PUb promoter. This discrepancy does not affect promoter function, and the plasmid should work as described.
growth strain :
Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Mlu1
cloning_site_5BamH1
promoterAedes polyubiquitin
sequencing_primer_3
sequencing_primer_5M13 reverse
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namedsRED
shRNA_sequence
size681
species
tags
plasmid copy :
Aedes aegypti PUb promoter and dsRED used in pSL1180polyUBdsRED was cloned using BamH1/Mlu1 from pMos-PUbDsRed-5HE-MCS-5HE as cited in Carpenetti et al. (2012) Insect Mol Biol. 21 : 97-106 (a kind gift of Dr Zach Adelman, Virginia Tech).
resistance markers :
555
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA