MSCV-myrAkt | Addgene 49267 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MSCV-myrAkt

catalog :

49267

citations: 2

Reference
Rasool R, O Connor C, Das C, Alhusayan M, Verma B, Islam S, et al. Loss of LCMT1 and biased protein phosphatase 2A heterotrimerization drive prostate cancer progression and therapy resistance. Nat Commun. 2023;14:5253 pubmed publisher
Kane L, Andres P, Howland K, Abbas A, Weiss A. Akt provides the CD28 costimulatory signal for up-regulation of IL-2 and IFN-gamma but not TH2 cytokines. Nat Immunol. 2001;2:37-44 pubmed

product information

Catalog Number :

49267

Product Name :

MSCV-myrAkt

article :

doi	10.1038/83144
id	7457
pubmed_id	11135576

bacterial resistance :

Ampicillin

cloning :

backbone

MSCV 2.2 IRES GFP

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

Please note that Addgene's sequencing results identified N2D, D149E and A478G mutations in the Akt sequence in this plasmid when compared to GenBank reference sequence AAW71957.1. These mutations were not intentional and the plasmid functions as described in the associated publication (i.e. it is constitutively active).

growth strain :

retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence

origin :

pi :

alt_names

AKT1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	BglII
promoter
sequencing_primer_3	pCDH-rev (GCATTCCTTTGGCGAGAG)
sequencing_primer_5	pLXSN-5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BOS_20453
gene	AKT1
id	280991

genbank_ids

mutation

N2D, D149E and A478G compared to AAW71957.1

name

Akt

shRNA_sequence

size

1500

species

9913

Bos taurus