pIRES2-EGFP-deltaNp53:WTp53 | Addgene 49244 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pIRES2-EGFP-deltaNp53:WTp53

catalog :

49244

citations: 1

Reference
Lin S, Karoly E, Taatjes D. The human ?Np53 isoform triggers metabolic and gene expression changes that activate mTOR and alter mitochondrial function. Aging Cell. 2013;12:863-72 pubmed publisher

product information

Catalog Number :

49244

Product Name :

pIRES2-EGFP-deltaNp53:WTp53

article :

doi	10.1111/acel.12108
id	7450
pubmed_id	23734707

bacterial resistance :

Kanamycin

cloning :

backbone

pIRES2-EGFP

backbone_mutation

backbone_origin

clonetch

backbone_size

5300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Note there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences are not known to affect function, though they may affect diagnostic digests.

growth strain :

expresses human p53 deltaN linked to full length p53 with C terminal His tag and also expresses EGFP in mammalian cells

origin :

pi :

alt_names

TP53

cloning

clone_method	Restriction Enzyme
cloning_site_3	KpnI
cloning_site_5	NheI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

name

p53 deltaN and p53 WT

shRNA_sequence

size

2343

species

9606

Homo sapiens