pET-15b-EK_C122S_His5 | Addgene 49048 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET-15b-EK_C122S_His5

catalog :

49048

citations: 3

Reference
Ly T, Moroz N, Pappas C, Novak S, Tolkatchev D, Wooldridge D, et al. The N-terminal tropomyosin- and actin-binding sites are important for leiomodin 2's function. Mol Biol Cell. 2016;27:2565-75 pubmed publisher
Colpan M, Tolkatchev D, Grover S, Helms G, Cort J, Moroz N, et al. Localization of the binding interface between leiomodin-2 and Î±-tropomyosin. Biochim Biophys Acta. 2016;1864:523-30 pubmed publisher
Skala W, Goettig P, Brandstetter H. Do-it-yourself histidine-tagged bovine enterokinase: a handy member of the protein engineer's toolbox. J Biotechnol. 2013;168:421-5 pubmed publisher

product information

Catalog Number :

49048

Product Name :

pET-15b-EK_C122S_His5

article :

doi	10.1016/j.jbiotec.2013.10.022
id	7809
pubmed_id	24184090

bacterial resistance :

Ampicillin

cloning :

backbone

pET-15b

backbone_mutation

backbone_origin

Novagen

backbone_size

5708

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth strain :

Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

origin :

pi :

alt_names

Enterokinase

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NcoI
promoter	T7
sequencing_primer_3	T7 term
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	PRSS7
gene	TMPRSS15
id	282009

genbank_ids

NM_174439.2

mutation

P92R, E185D, C122S

name

Enteropeptidase, peptidase domain

shRNA_sequence

size

species

9913

Bos taurus