pCMV_dCas9_VP64 | Addgene 49015 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV_dCas9_VP64

catalog :

49015

citations: 4

Reference
Zhang C, Zhang Y, Zhang W, Tong H, Li S, Yan Y. WISP1 promotes bovine MDSC differentiation via recruitment of ANXA1 for the regulation of the TGF-β signalling pathway. Mol Cell Biochem. 2020;470:215-227 pubmed publisher
McDonald J, Celik H, Rois L, Fishberger G, Fowler T, Rees R, et al. Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation. Biol Open. 2016;5:866-74 pubmed publisher
Jusiak B, Cleto S, Perez PiÃ±era P, Lu T. Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Trends Biotechnol. 2016;34:535-547 pubmed publisher
Farzadfard F, Perli S, Lu T. Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas. ACS Synth Biol. 2013;2:604-13 pubmed publisher

product information

Catalog Number :

49015

Product Name :

pCMV_dCas9_VP64

article :

doi	10.1021/sb400081r
id	7373
pubmed_id	23977949

bacterial resistance :

Ampicillin

cloning :

backbone

phi-Yellow-Dest

backbone_mutation

Inserted dCas9-3xNLS-VP64 along with pPGK1_mKATE-HSVpolyA

backbone_origin

Evrogen

backbone_size

4000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

CRISPR

Synthetic Biology

growth notes :

L591M mutation with dCas9

growth strain :

encodes human-optimized dCas9_VP64 synthetic transcription factor

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	AGTGCGGCGACGATAGTCATGCCCC
sequencing_primer_5	TCTTCGCTATTACGCCAGCCC
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

dCas9_VP64 (human-codon-optimized)

shRNA_sequence

size

4410

species

9606

Homo sapiens