This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTPGI_dCas9_VP64
catalog :
49013
citations: 7
Reference
Brion C, Lutz S, ALBERT F. Simultaneous quantification of mRNA and protein in single cells reveals post-transcriptional effects of genetic variation. elife. 2020;9: pubmed publisher
Donovan D, Crandall J, Banks O, Jensvold Z, Truong V, Dinwiddie D, et al. Engineered Chromatin Remodeling Proteins for Precise Nucleosome Positioning. Cell Rep. 2019;29:2520-2535.e4 pubmed publisher
Li P, Fu X, Zhang L, Li S. CRISPR/Cas-based screening of a gene activation library in Saccharomyces cerevisiae identifies a crucial role of OLE1 in thermotolerance. Microb Biotechnol. 2018;: pubmed publisher
Li J, Xu Z, Chupalov A, Marchisio M. Anti-CRISPR-based biosensors in the yeast S. cerevisiae. J Biol Eng. 2018;12:11 pubmed publisher
Chen Y, Farzadfard F, Gharaei N, Chen W, Cao J, Lu T. Randomized CRISPR-Cas Transcriptional Perturbation Screening Reveals Protective Genes against Alpha-Synuclein Toxicity. Mol Cell. 2017;68:247-257.e5 pubmed publisher
Jusiak B, Cleto S, Perez Piñera P, Lu T. Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Trends Biotechnol. 2016;34:535-547 pubmed publisher
Farzadfard F, Perli S, Lu T. Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas. ACS Synth Biol. 2013;2:604-13 pubmed publisher
product information
Catalog Number :
49013
Product Name :
pTPGI_dCas9_VP64
article :
doi10.1021/sb400081r
id7373
pubmed_id23977949
bacterial resistance :
Ampicillin
cloning :
backbonepTPGI (pRS304)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
CRISPR
Synthetic Biology
growth strain :
encodes yeast-optimized dCas9_VP64 synthetic transcription factor
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BamHI
promoterpTPGI (galactose+aTc inducible)
sequencing_primer_3
sequencing_primer_5GCCTCGCGCCGCACTGCTCC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namedCas9_VP64 (codon-optimized for expression in S. cerevisiae)
shRNA_sequence
size4335
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagSV40 NLS
resistance markers :
1726
tags :
High Copy
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA