This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3 HA-Sumo1 WT
catalog :
48966
citations: 4
Reference
Yamada S, Sato A, Ishihara N, Akiyama H, Sakakibara S. Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development. iScience. 2021;24:103484 pubmed publisher
Choi J, Fan C, Kim D, Sharif M, An H, Park Y. Elucidating the transactivation domain of the pleiotropic transcription factor Myrf. Sci Rep. 2018;8:13075 pubmed publisher
Hoard T, Yang X, Jetten A, Zeruth G. PIAS-family proteins negatively regulate Glis3 transactivation function through SUMO modification in pancreatic β cells. Heliyon. 2018;4:e00709 pubmed publisher
Bekes M, Prudden J, Srikumar T, Raught B, Boddy M, Salvesen G. The dynamics and mechanism of SUMO chain deconjugation by SUMO-specific proteases. J Biol Chem. 2011;286:10238-47 pubmed publisher
product information
Catalog Number :
48966
Product Name :
pcDNA3 HA-Sumo1 WT
article :
doi10.1074/jbc.M110.205153
id5308
pubmed_id21247896
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note this plasmid contains the mature form of SUMO1, which lacks the last 4 amino acids from the C-terminus.
growth strain :
Expresses HA-Sumo1 WT in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5HindIII
promoterT7
sequencing_primer_3BGH Reverse
sequencing_primer_5T7 Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDAP1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, UBL1
geneSUMO1
id7341
genbank_ids
mutation
nameSUMO1
shRNA_sequence
size327
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
435
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA