This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSpCas9n(BB) (PX460)
catalog :
48873
citations: 9
Reference
Malik R, Svoboda P. CRISPR-Induced Expression of N-Terminally Truncated Dicer in Mouse Cells. Genes (Basel). 2021;12: pubmed publisher
Benito Kwiecinski S, Giandomenico S, Sutcliffe M, Riis E, Freire Pritchett P, Kelava I, et al. An early cell shape transition drives evolutionary expansion of the human forebrain. Cell. 2021;184:2084-2102.e19 pubmed publisher
Arnoult N, Correia A, Ma J, Merlo A, Garcia Gomez S, Maric M, et al. Regulation of DNA repair pathway choice in S and G2 phases by the NHEJ inhibitor CYREN. Nature. 2017;549:548-552 pubmed publisher
He Z, Chen L, Souto F, Canasto Chibuque C, Bongers G, Deshpande M, et al. Epithelial-derived IL-33 promotes intestinal tumorigenesis in Apc Min/+ mice. Sci Rep. 2017;7:5520 pubmed publisher
Koehler K, Nie J, Longworth Mills E, Liu X, Lee J, Holt J, et al. Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells. Nat Biotechnol. 2017;35:583-589 pubmed publisher
Santos D, Kiskinis E, Eggan K, Merkle F. Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Curr Protoc Stem Cell Biol. 2016;38:5B.6.1-5B.6.60 pubmed publisher
Domingues P, Bamford C, Boutell C, McLauchlan J. Inhibition of hepatitis C virus RNA replication by ISG15 does not require its conjugation to protein substrates by the HERC5 E3 ligase. J Gen Virol. 2015;96:3236-42 pubmed
Bialk P, Rivera Torres N, Strouse B, Kmiec E. Regulation of Gene Editing Activity Directed by Single-Stranded Oligonucleotides and CRISPR/Cas9 Systems. PLoS ONE. 2015;10:e0129308 pubmed publisher
Ran F, Hsu P, Wright J, Agarwala V, Scott D, Zhang F. Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013;8:2281-2308 pubmed publisher
product information
Catalog Number :
48873
Product Name :
pSpCas9n(BB) (PX460)
article :
doi10.1038/nprot.2013.143
id7475
pubmed_id24157548
bacterial resistance :
Ampicillin
cloning :
backbonePX460
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/.
growth strain :
Cas9n (D10A nickase mutant) from S. pyogenes
origin :
37
pi :
alt_names
SpCas9n
Cas9n
PX460
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoterCbh
sequencing_primer_3
sequencing_primer_5TTTATGGCGAGGCGGCGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationCas9 D10A nickase mutant
namehSpCas9n
shRNA_sequence
size5400
species
100
Synthetic
tags
locationN terminal on insert
tag3XFLAG
resistance markers :
898
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA