This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDNA3.1-nAChr-beta3
catalog :
48811
citations: 1
Reference
Drenan R, Nashmi R, Imoukhuede P, Just H, McKinney S, Lester H. Subcellular trafficking, pentameric assembly, and subunit stoichiometry of neuronal nicotinic acetylcholine receptors containing fluorescently labeled alpha6 and beta3 subunits. Mol Pharmacol. 2008;73:27-41 pubmed
product information
Catalog Number :
48811
Product Name :
pCDNA3.1-nAChr-beta3
article :
doi10.1124/mol.107.039180
id7337
pubmed_id17932221
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originLife Technologies
backbone_size5597
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
*Compared to the NCBI Genbank Entrez entry for nAChr-beta3, this plasmid has a K107R amino acid residue substitution. See accession ID: AAL75573.1 AF467896_1 for the correct reference sequence.
growth strain :
This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of 6- and 3-containing receptors using TIRF.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5EcoRV
promoterCMV
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed1
entrez_gene
aliases5730417K16Rik, Acrb3
geneChrnb3
id108043
genbank_ids
AAL75573.1
AF467896_1
mutation*
namenAChr Beta 3 subunit, isoform 1
shRNA_sequence
size1530
species
10090
Mus musculus
tags
resistance markers :
288
1757
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA