This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET-UNC60B
catalog :
48740
citations: 1
Reference
Ono S, Benian G. Two Caenorhabditis elegans actin depolymerizing factor/cofilin proteins, encoded by the unc-60 gene, differentially regulate actin filament dynamics. J Biol Chem. 1998;273:3778-83 pubmed
product information
Catalog Number :
48740
Product Name :
pET-UNC60B
article :
doi10.1074/jbc.273.6.3778
id7314
pubmed_id9452511
bacterial resistance :
Ampicillin
cloning :
backbonepET-3d
backbone_mutation
backbone_originNovagen
backbone_size459
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Please see the insert sequence provided by the depositing laboratory and Figure 1 from the associated publication (Ono S and Benian GM, JBC 1998) listed below for information on the UNC-60 isoform in this plasmid. This plasmid encodes the UNC-60B isoform as described in the publication and now referred to as UNC-60C by NCBI and Wormbase (GenBank reference sequence NP_503427.2). The original nomenclature of UNC-60A and UNC-60B was defined in the publication Genetic organization of the unc-60 region in Caenorhabditis elegans. McKim KS, Heschl MF, Rosenbluth RE, Baillie DL. Genetics. 1988 Jan;118(1):49-59. https://www.ncbi.nlm.nih.gov/pubmed/8608931
growth strain :
Bacterial expression of UNC-60B (a C. elegans ADF/cofilin)
origin :
37
pi :
alt_names
C. elegans ADF/cofilin
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NcoI
promoterT7
sequencing_primer_3T7term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_C38C3.5
geneunc-60
id178640
genbank_ids
mutation
nameC. elegans UNC-60B cDNA
shRNA_sequence
size459
species
6239
Caenorhabditis elegans
tags
resistance markers :
1711
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA