This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBabe(puro)-Omi-mCherry
catalog :
48685
citations: 1
Reference
Tait S, PARSONS M, Llambi F, Bouchier Hayes L, Connell S, Munoz Pinedo C, et al. Resistance to caspase-independent cell death requires persistence of intact mitochondria. Dev Cell. 2010;18:802-13 pubmed publisher
product information
Catalog Number :
48685
Product Name :
pBabe(puro)-Omi-mCherry
article :
doi10.1016/j.devcel.2010.03.014
id5955
pubmed_id20493813
bacterial resistance :
Ampicillin
cloning :
backbonepBabe (puro)
backbone_mutationmCherry cloned via 5 BamH1/3 EcoRI restriction digest into pBabe puro. Human Omi (aka HtrA2) cloned via PCR/restriction digest using Omi-TC vector as template and primers with BglII restriction sites. PCR product was cut with BglII and cloned into pBabe mCherry via the BamHI site.
backbone_origin
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
growth strain :
Expresses fusion protein in mitochondria inter-membrane space, and is released upon MOMP.
origin :
30
pi :
alt_names
HTRA2
HtrA serine peptidase 2
PARK13
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoterLTR
sequencing_primer_33'-accctaactgacacacattcc
sequencing_primer_55'-ctttatccagccctcac
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesMGCA8, OMI, PARK13, PRSS25
geneHTRA2
id27429
genbank_ids
NM_013247.4
mutation
nameOmi
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmCherry
resistance markers :
1374
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA