This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.BRWD3-V5H
catalog :
48624
citations: 1
Reference
Ozturk N, VanVickle Chavez S, Akileswaran L, Van Gelder R, Sancar A. Ramshackle (Brwd3) promotes light-induced ubiquitylation of Drosophila Cryptochrome by DDB1-CUL4-ROC1 E3 ligase complex. Proc Natl Acad Sci U S A. 2013;110:4980-5 pubmed publisher
product information
Catalog Number :
48624
Product Name :
pcDNA3.BRWD3-V5H
article :
doi10.1073/pnas.1303234110
id7276
pubmed_id23479607
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses Drosophila BRWD3 (with V5 and His tags at the C-terminus) in mammalian cells
origin :
37
pi :
alt_names
Ramshackle
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRV
cloning_site_5KpnI
promoterCMV
sequencing_primer_3BGH Rev
sequencing_primer_5T7 for
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesDmel_CG31132, CG18670, CG31132, CG6400, Dmel CG31132, anon-WO0118547.520, anon-WO0118547.620, dBRWD3, l(3)05842, l(3)s5349, ram
geneBRWD3
id42898
genbank_ids
mutationno mutations, stop was removed to fuse V5His at C-ter.
nameBRWD3
shRNA_sequence
size6808
species
7227
Drosophila melanogaster
tags
locationC terminal on insert
tagV5 and His tags
resistance markers :
436
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA