This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSpCas9n(BB)-2A-Puro (PX462)
catalog :
48141
citations: 57
Reference
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Yu Y, Suryo Rahmanto Y, Shen Y, Ardighieri L, Davidson B, Gaillard S, et al. Spleen tyrosine kinase activity regulates epidermal growth factor receptor signaling pathway in ovarian cancer. EBioMedicine. 2019;47:184-194 pubmed publisher
Diaz Osterman C, Ozmadenci D, Kleinschmidt E, Taylor K, Barrie A, Jiang S, et al. FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy. elife. 2019;8: pubmed publisher
Alateeq S, Ovchinnikov D, Tracey T, Whitworth D, Al Rubaish A, Al Ali A, et al. Identification of on-target mutagenesis during correction of a beta-thalassemia splice mutation in iPS cells with optimised CRISPR/Cas9-double nickase reveals potential safety concerns. APL Bioeng. 2018;2:046103 pubmed publisher
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Yu Y, Suryo Rahmanto Y, Lee M, Wu P, Phillip J, Huang C, et al. Inhibition of ovarian tumor cell invasiveness by targeting SYK in the tyrosine kinase signaling pathway. Oncogene. 2018;37:3778-3789 pubmed publisher
Sun H, Lagarrigue F, Gingras A, Fan Z, Ley K, Ginsberg M. Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development. J Cell Biol. 2018;217:1453-1465 pubmed publisher
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Zhou X, Zou X, Chung H, Gao Y, Liu Y, Qi L, et al. A Single-Chain Photoswitchable CRISPR-Cas9 Architecture for Light-Inducible Gene Editing and Transcription. ACS Chem Biol. 2018;13:443-448 pubmed publisher
Ge Y, Paisie T, Newman J, McIntyre L, Concannon P. UBASH3A Mediates Risk for Type 1 Diabetes Through Inhibition of T-Cell Receptor-Induced NF-κB Signaling. Diabetes. 2017;66:2033-2043 pubmed publisher
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Lehmann J, Seebode C, Smolorz S, Schubert S, Emmert S. XPF knockout via CRISPR/Cas9 reveals that ERCC1 is retained in the cytoplasm without its heterodimer partner XPF. Cell Mol Life Sci. 2017;74:2081-2094 pubmed publisher
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Horibata Y, Ando H, Zhang P, Vergnes L, Aoyama C, Itoh M, et al. StarD7 Protein Deficiency Adversely Affects the Phosphatidylcholine Composition, Respiratory Activity, and Cristae Structure of Mitochondria. J Biol Chem. 2016;291:24880-24891 pubmed
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Richter B, Sliter D, Herhaus L, Stolz A, Wang C, Beli P, et al. Phosphorylation of OPTN by TBK1 enhances its binding to Ub chains and promotes selective autophagy of damaged mitochondria. Proc Natl Acad Sci U S A. 2016;113:4039-44 pubmed publisher
Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, et al. Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res. 2016;44:5615-28 pubmed publisher
Nielsen C, Huttner D, Bizard A, Hirano S, Li T, Palmai Pallag T, et al. PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis. Nat Commun. 2015;6:8962 pubmed publisher
Flynn R, Grundmann A, Renz P, Hänseler W, James W, Cowley S, et al. CRISPR-mediated genotypic and phenotypic correction of a chronic granulomatous disease mutation in human iPS cells. Exp Hematol. 2015;43:838-848.e3 pubmed publisher
Kelly S, Georgomanolis T, Zirkel A, Diermeier S, O Reilly D, Murphy S, et al. Splicing of many human genes involves sites embedded within introns. Nucleic Acids Res. 2015;43:4721-32 pubmed publisher
Ran F, Hsu P, Wright J, Agarwala V, Scott D, Zhang F. Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013;8:2281-2308 pubmed publisher
product information
Catalog Number :
48141
Product Name :
pSpCas9n(BB)-2A-Puro (PX462)
article :
doi10.1038/nprot.2013.143
id7475
pubmed_id24157548
bacterial resistance :
Ampicillin
cloning :
backbonePX462
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/.
growth strain :
NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987
origin :
37
pi :
alt_names
SpCas9n-2A-Puro
Cas9n
PX462
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoterCbh
sequencing_primer_3
sequencing_primer_5TTTATGGCGAGGCGGCGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationCas9 D10A nickase mutant
namehSpCas9n-2A-Puro
shRNA_sequence
size6000
species
100
Synthetic
tags
locationN terminal on insert
tag3XFLAG
locationC terminal on insert
tag2A-Puro
resistance markers :
898
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA