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product summary
company name :
Addgene
product type :
cDNA
product name :
pSpCas9n(BB)-2A-GFP (PX461)
catalog :
48140
citations: 126
Reference
Li W, Zhang S, Peng W, Shi Y, Yuan B, Yuan Y, et al. Whole-brain in vivo base editing reverses behavioral changes in Mef2c-mutant mice. Nat Neurosci. 2024;27:116-128 pubmed publisher
Pinto L, Pailas A, Bondarchenko M, Sharma A, Neumann K, Rizzo A, et al. DAXX promotes centromeric stability independently of ATRX by preventing the accumulation of R-loop-induced DNA double-stranded breaks. Nucleic Acids Res. 2023;: pubmed publisher
Aboulkassim T, Tian X, Liu Q, Qiu D, Hancock M, Wu J, et al. A NRF2 inhibitor selectively sensitizes KEAP1 mutant tumor cells to cisplatin and gefitinib by restoring NRF2-inhibitory function of KEAP1 mutants. Cell Rep. 2023;42:113104 pubmed publisher
Irmer B, Efing J, Reitnauer L, Angenendt A, Heinrichs S, Schubert A, et al. Extracellular vesicle-associated tyrosine kinase-like orphan receptors ROR1 and ROR2 promote breast cancer progression. Cell Commun Signal. 2023;21:171 pubmed publisher
Li D, Ou M, Zhang W, Luo Q, Cai W, Mo C, et al. CRISPR/Cas9-Mediated Gene Correction in Osteopetrosis Patient-Derived iPSCs. Front Biosci (Landmark Ed). 2023;28:131 pubmed publisher
van der Mijn J, Laursen K, Fu L, Khani F, Dow L, Nowak D, et al. Novel genetically engineered mouse models for clear cell renal cell carcinoma. Sci Rep. 2023;13:8246 pubmed publisher
Amhaz S, Bo xeb da B, Chouch xe8 ne M, Colasse S, Dingli F, Loew D, et al. The UAS thioredoxin-like domain of UBXN7 regulates E3 ubiquitin ligase activity of RNF111/Arkadia. BMC Biol. 2023;21:73 pubmed publisher
Liu J, Zhang C, Xu D, Zhang T, Chang C, Wang J, et al. The ubiquitin ligase TRIM21 regulates mutant p53 accumulation and gain of function in cancer. J Clin Invest. 2023;133: pubmed publisher
Zhang S, Song L, Yuan B, Zhang C, Cao J, Chen J, et al. TadA reprogramming to generate potent miniature base editors with high precision. Nat Commun. 2023;14:413 pubmed publisher
Liu J, Zhang C, Zhang T, Chang C, Wang J, Bazile L, et al. Metabolic enzyme LDHA activates Rac1 GTPase as a noncanonical mechanism to promote cancer. Nat Metab. 2022;4:1830-1846 pubmed publisher
Hosios A, Wilkinson M, McNamara M, Kalafut K, Torrence M, Asara J, et al. mTORC1 regulates a lysosome-dependent adaptive shift in intracellular lipid species. Nat Metab. 2022;4:1792-1811 pubmed publisher
Bahrami Nejad Z, Zhang Z, Tholen S, Sharma S, Rabiee A, Zhao M, et al. Early enforcement of cell identity by a functional component of the terminally differentiated state. PLoS Biol. 2022;20:e3001900 pubmed publisher
Pardal A, Bowman A. A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3. elife. 2022;11: pubmed publisher
De Angeli P, Reuter P, Hauser S, Sch xf6 ls L, Stingl K, Wissinger B, et al. Effective splicing restoration of a deep-intronic ABCA4 variant in cone photoreceptor precursor cells by CRISPR/SpCas9 approaches. Mol Ther Nucleic Acids. 2022;29:511-524 pubmed publisher
Dibble C, Barritt S, Perry G, Lien E, Geck R, DuBois Coyne S, et al. PI3K drives the de novo synthesis of coenzyme A from vitamin B5. Nature. 2022;608:192-198 pubmed publisher
Lu Y, Mu Y, Chen J, Guan X, Guo L, Wu C. CLP36 promotes p53 deficient sarcoma progression through suppression of atrophin-1 interacting protein-4 (AIP-4)-dependent degradation of YAP1. Theranostics. 2022;12:5051-5068 pubmed publisher
Lin X, Swedlund B, Ton M, Ghazanfar S, Guibentif C, Paulissen C, et al. Mesp1 controls the chromatin and enhancer landscapes essential for spatiotemporal patterning of early cardiovascular progenitors. Nat Cell Biol. 2022;24:1114-1128 pubmed publisher
Chen Q, Bates A, Hanquier J, Simpson E, Rusch D, Podicheti R, et al. Structural and genome-wide analyses suggest that transposon-derived protein SETMAR alters transcription and splicing. J Biol Chem. 2022;298:101894 pubmed publisher
Prijic S, Chang J. ABCA1 Expression Is Upregulated in an EMT in Breast Cancer Cell Lines via MYC-Mediated De-Repression of Its Proximal Ebox Element. Biomedicines. 2022;10: pubmed publisher
Jachowicz J, Strehle M, Banerjee A, Blanco M, Thai J, Guttman M. Xist spatially amplifies SHARP/SPEN recruitment to balance chromosome-wide silencing and specificity to the X chromosome. Nat Struct Mol Biol. 2022;29:239-249 pubmed publisher
Xu M, Zhao Y, Zhang W, Geng M, Liu Q, Gao Q, et al. Genome-scale screening in a rat haploid system identifies Thop1 as a modulator of pluripotency exit. Cell Prolif. 2022;55:e13209 pubmed publisher
Lee D, Jeong Y, Won J, Sim H, Park Y, Jin H. PBK/TOPK Is a Favorable Prognostic Biomarker Correlated with Antitumor Immunity in Colon Cancers. Biomedicines. 2022;10: pubmed publisher
Leary N, Walser S, He Y, Cousin N, Pereira P, Gallo A, et al. Melanoma-derived extracellular vesicles mediate lymphatic remodelling and impair tumour immunity in draining lymph nodes. J Extracell Vesicles. 2022;11:e12197 pubmed publisher
Oh C, Dolatabadi N, Cieplak P, Diaz Meco M, Moscat J, Nolan J, et al. S-Nitrosylation of p62 Inhibits Autophagic Flux to Promote α-Synuclein Secretion and Spread in Parkinson's Disease and Lewy Body Dementia. J Neurosci. 2022;42:3011-3024 pubmed publisher
Diaz R, Kronenberg N, Martinelli A, Liehm P, Riches A, Gather M, et al. KIAA0319 influences cilia length, cell migration and mechanical cell-substrate interaction. Sci Rep. 2022;12:722 pubmed publisher
Rodriguez E, Boelaars K, Brown K, Madunić K, van Ee T, Dijk F, et al. Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits. Commun Biol. 2022;5:41 pubmed publisher
Zheng D, Wang X, Zhang Z, Li E, Yeung C, Borkar R, et al. Engineering of human mesenchymal stem cells resistant to multiple natural killer subtypes. Int J Biol Sci. 2022;18:426-440 pubmed publisher
Nitzahn M, Truong B, Khoja S, Vega Crespo A, Le C, Eliav A, et al. CRISPR-Mediated Genomic Addition to CPS1 Deficient iPSCs is Insufficient to Restore Nitrogen Homeostasis. Yale J Biol Med. 2021;94:545-557 pubmed
Wilk C, Effenberg L, Abberger H, Steenpass L, Hansen W, Zeschnigk M, et al. CRISPR/Cas9-mediated demethylation of FOXP3-TSDR toward Treg-characteristic programming of Jurkat T cells. Cell Immunol. 2021;371:104471 pubmed publisher
Rahman M, Hyodo T, Karnan S, Ota A, Hasan M, Mihara Y, et al. Experimental strategies to achieve efficient targeted knock-in via tandem paired nicking. Sci Rep. 2021;11:22627 pubmed publisher
Earley A, Burbulla L, Krainc D, Awatramani R. Identification of ASCL1 as a determinant for human iPSC-derived dopaminergic neurons. Sci Rep. 2021;11:22257 pubmed publisher
Park S, Kim Y, Ra J, Wie M, Kang M, Kang S, et al. Timely termination of repair DNA synthesis by ATAD5 is important in oxidative DNA damage-induced single-strand break repair. Nucleic Acids Res. 2021;49:11746-11764 pubmed publisher
Smith L, Ladner J, Hodara V, Parodi L, Harris R, Callery J, et al. Multiplexed Simian Immunodeficiency Virus-Specific Paired RNA-Guided Cas9 Nickases Inactivate Proviral DNA. J Virol. 2021;95:e0088221 pubmed publisher
de Jong J, Llapashtica C, Genestine M, Strauss K, Provenzano F, Sun Y, et al. Cortical overgrowth in a preclinical forebrain organoid model of CNTNAP2-associated autism spectrum disorder. Nat Commun. 2021;12:4087 pubmed publisher
Santa Inez D, Fuziwara C, Saito K, Kimura E. Targeting the Highly Expressed microRNA miR-146b with CRISPR/Cas9n Gene Editing System in Thyroid Cancer. Int J Mol Sci. 2021;22: pubmed publisher
Lee H, Lee I, Kim J, Lee S, Kwak S, Suh M, et al. Phosphorylation of OGFOD1 by Cell Cycle-Dependent Kinase 7/9 Enhances the Transcriptional Activity of RNA Polymerase II in Breast Cancer Cells. Cancers (Basel). 2021;13: pubmed publisher
Chen K, Gorgen A, Ding A, Du L, Jiang K, Ding Y, et al. Dual-Specificity Phosphatase 9 Regulates Cellular Proliferation and Predicts Recurrence After Surgery in Hepatocellular Carcinoma. Hepatol Commun. 2021;5:1310-1328 pubmed publisher
Xu Y, Guo C, Pan W, Zhao C, Ding Y, Xie X, et al. Nephrotic syndrome-associated mutation of KANK2 induces pathologic binding competition with physiological interactor KIF21A. J Biol Chem. 2021;:100958 pubmed publisher
Klouwer F, Falkenberg K, Ofman R, Koster J, van Gent D, Ferdinandusse S, et al. Autophagy Inhibitors Do Not Restore Peroxisomal Functions in Cells With the Most Common Peroxisome Biogenesis Defect. Front Cell Dev Biol. 2021;9:661298 pubmed publisher
Liao Z, Gingras A, Lagarrigue F, Ginsberg M, Shattil S. Optogenetics-based localization of talin to the plasma membrane promotes activation of β3 integrins. J Biol Chem. 2021;296:100675 pubmed publisher
Kim S, Wong Y, Gao F, Krainc D. Dysregulation of mitochondria-lysosome contacts by GBA1 dysfunction in dopaminergic neuronal models of Parkinson's disease. Nat Commun. 2021;12:1807 pubmed publisher
Jackson M, Fidanza A, Taylor A, Rybtsov S, Axton R, Kydonaki M, et al. Modulation of APLNR Signaling Is Required during the Development and Maintenance of the Hematopoietic System. Stem Cell Reports. 2021;16:727-740 pubmed publisher
Sayedyahossein S, Huang K, Li Z, Zhang C, Kozlov A, Johnston D, et al. Pannexin 1 binds β-catenin to modulate melanoma cell growth and metabolism. J Biol Chem. 2021;296:100478 pubmed publisher
Tang Y, Liao S, Liu G, Xiong X, Liu H, Li F, et al. Advanced single-cell pooled CRISPR screening identifies C19orf53 required for cell proliferation based on mTORC1 regulators. Cell Biol Toxicol. 2021;: pubmed publisher
Ye T, Duan Y, Tsang H, Xu H, Chen Y, Cao H, et al. Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases. Commun Biol. 2021;4:195 pubmed publisher
Nouri Nejad D, O Donnell B, Patil C, Sanchez Pupo R, Johnston D, Sayedyahossein S, et al. Pannexin 1 mutation found in melanoma tumour reduces phosphorylation, glycosylation, and trafficking of the channel-forming protein. Mol Biol Cell. 2021;:mbcE19100585 pubmed publisher
Kato M, Abdollahi M, Tunduguru R, Tsark W, Chen Z, Wu X, et al. miR-379 deletion ameliorates features of diabetic kidney disease by enhancing adaptive mitophagy via FIS1. Commun Biol. 2021;4:30 pubmed publisher
Li S, Yuan B, Cao J, Chen J, Chen J, Qiu J, et al. Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination. Nat Commun. 2020;11:5827 pubmed publisher
Pinzaru A, Kareh M, Lamm N, Lazzerini Denchi E, Cesare A, Sfeir A. Replication stress conferred by POT1 dysfunction promotes telomere relocalization to the nuclear pore. Genes Dev. 2020;34:1619-1636 pubmed publisher
Ito F, Yanatori I, Maeda Y, Nimura K, Ito S, Hirayama T, et al. Asbestos conceives Fe(II)-dependent mutagenic stromal milieu through ceaseless macrophage ferroptosis and β-catenin induction in mesothelium. Redox Biol. 2020;36:101616 pubmed publisher
Lee M, Choi N, Park S, Bang J, Lee Y, Jeong D, et al. Generation of OCT4-EGFP, NANOG-tdTomato dual reporter human induced pluripotent stem cell line, KKUi001-A, using the CRISPR/Cas9 system. Stem Cell Res. 2020;48:101943 pubmed publisher
Bu W, Levitskaya Z, Loh Z, Jin S, Basu S, Ero R, et al. Structural basis of human full-length kindlin-3 homotrimer in an auto-inhibited state. PLoS Biol. 2020;18:e3000755 pubmed publisher
Brannvoll A, Xue X, Kwon Y, Kompocholi S, Simonsen A, Viswalingam K, et al. The ZGRF1 Helicase Promotes Recombinational Repair of Replication-Blocking DNA Damage in Human Cells. Cell Rep. 2020;32:107849 pubmed publisher
Ikeda M, Taniguchi Ikeda M, Kato T, Shinkai Y, Tanaka S, Hagiwara H, et al. Unexpected Mutations by CRISPR-Cas9 CTG Repeat Excision in Myotonic Dystrophy and Use of CRISPR Interference as an Alternative Approach. Mol Ther Methods Clin Dev. 2020;18:131-144 pubmed publisher
Harms F, Parthasarathy P, Zorndt D, Alawi M, Fuchs S, Halliday B, et al. Biallelic loss-of-function variants in TBC1D2B cause a neurodevelopmental disorder with seizures and gingival overgrowth. Hum Mutat. 2020;41:1645-1661 pubmed publisher
Liu J, Zhang C, Wu H, Sun X, Li Y, Huang S, et al. Parkin ubiquitinates phosphoglycerate dehydrogenase to suppress serine synthesis and tumor progression. J Clin Invest. 2020;130:3253-3269 pubmed publisher
Cruz Becerra G, Kadonaga J. Enhancement of homology-directed repair with chromatin donor templates in cells. elife. 2020;9: pubmed publisher
An J, Kinney C, Tan J, Liao G, Kremer E, Xu B. TrkB-expressing paraventricular hypothalamic neurons suppress appetite through multiple neurocircuits. Nat Commun. 2020;11:1729 pubmed publisher
Patkunarajah A, Stear J, Moroni M, Schroeter L, Blaszkiewicz J, Tearle J, et al. TMEM87a/Elkin1, a component of a novel mechanoelectrical transduction pathway, modulates melanoma adhesion and migration. elife. 2020;9: pubmed publisher
Pramfalk C, Jakobsson T, Verzijl C, Minniti M, Obensa C, Ripamonti F, et al. Generation of new hepatocyte-like in vitro models better resembling human lipid metabolism. Biochim Biophys Acta Mol Cell Biol Lipids. 2020;1865:158659 pubmed publisher
Park S, Kang N, Song E, Wie M, Lee E, Hwang S, et al. ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress. Nat Commun. 2019;10:5718 pubmed publisher
Zhang T, Wallis M, Petrovic V, Challis J, Kalitsis P, Hudson D. Loss of TOP3B leads to increased R-loop formation and genome instability. Open Biol. 2019;9:190222 pubmed publisher
Wang X, Rusin A, Walkey C, Lin J, Johnson D. The RNA polymerase III repressor MAF1 is regulated by ubiquitin-dependent proteasome degradation and modulates cancer drug resistance and apoptosis. J Biol Chem. 2019;294:19255-19268 pubmed publisher
Graham M, Kim J, Da J, Brosnan Cashman J, Rizzo A, Baena Del Valle J, et al. Functional Loss of ATRX and TERC Activates Alternative Lengthening of Telomeres (ALT) in LAPC4 Prostate Cancer Cells. Mol Cancer Res. 2019;17:2480-2491 pubmed publisher
Li J, Ji H, Porter D, Broude E, Roninson I, Chen M. Characterizing CDK8/19 Inhibitors through a NFκB-Dependent Cell-Based Assay. Cells. 2019;8: pubmed publisher
Chen J, Cao Y, Markelc B, Kaeppler J, Vermeer J, Muschel R. Type I IFN protects cancer cells from CD8+ T cell-mediated cytotoxicity after radiation. J Clin Invest. 2019;129:4224-4238 pubmed publisher
Menges J, Cremanns M, Steenpass L. Generation of two H1 hESC sublines carrying deletions of RB1 exon 1/promoter in heterozygous or compound heterozygous state. Stem Cell Res. 2019;39:101517 pubmed publisher
Cheng T, Li S, Yuan B, Wang X, Zhou W, Qiu Z. Expanding C-T base editing toolkit with diversified cytidine deaminases. Nat Commun. 2019;10:3612 pubmed publisher
Ellefsen K, Holt J, Chang A, Nourse J, Arulmoli J, Mekhdjian A, et al. Myosin-II mediated traction forces evoke localized Piezo1-dependent Ca2+ flickers. Commun Biol. 2019;2:298 pubmed publisher
Dieterich L, Kapaklikaya K, Cetintas T, Proulx S, Commerford C, Ikenberg K, et al. Transcriptional profiling of breast cancer-associated lymphatic vessels reveals VCAM-1 as regulator of lymphatic invasion and permeability. Int J Cancer. 2019;145:2804-2815 pubmed publisher
Diquigiovanni C, Bergamini C, Diaz R, Liparulo I, Bianco F, Masin L, et al. A novel mutation in SPART gene causes a severe neurodevelopmental delay due to mitochondrial dysfunction with complex I impairments and altered pyruvate metabolism. FASEB J. 2019;:fj201802722R pubmed publisher
Gregersen L, Mitter R, Ugalde A, Nojima T, Proudfoot N, Agami R, et al. SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Cell. 2019;: pubmed publisher
Alateeq S, Ovchinnikov D, Tracey T, Whitworth D, Al Rubaish A, Al Ali A, et al. Identification of on-target mutagenesis during correction of a beta-thalassemia splice mutation in iPS cells with optimised CRISPR/Cas9-double nickase reveals potential safety concerns. APL Bioeng. 2018;2:046103 pubmed publisher
Colognori D, Sunwoo H, Kriz A, Wang C, Lee J. Xist Deletional Analysis Reveals an Interdependency between Xist RNA and Polycomb Complexes for Spreading along the Inactive X. Mol Cell. 2019;74:101-117.e10 pubmed publisher
Baron D, Matheny T, Lin Y, Leszyk J, Kenna K, Gall K, et al. Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS. Hum Mol Genet. 2019;: pubmed publisher
Bäck S, Necarsulmer J, Whitaker L, Coke L, Koivula P, Heathward E, et al. Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats. Neuron. 2019;102:105-119.e8 pubmed publisher
Heyn P, Logan C, Fluteau A, Challis R, Auchynnikava T, Martin C, et al. Gain-of-function DNMT3A mutations cause microcephalic dwarfism and hypermethylation of Polycomb-regulated regions. Nat Genet. 2019;51:96-105 pubmed publisher
Smythies J, Sun M, Masson N, Salama R, Simpson P, Murray E, et al. Inherent DNA-binding specificities of the HIF-1α and HIF-2α transcription factors in chromatin. EMBO Rep. 2019;20: pubmed publisher
Reilly M, Stokman M, Magry V, Jeanpierre C, Alves M, Paydar M, et al. Loss-of-function mutations in KIF14 cause severe microcephaly and kidney development defects in humans and zebrafish. Hum Mol Genet. 2019;28:778-795 pubmed publisher
Yang J, Park J, Zheng D, Xu R. Universal Corneal Epithelial-Like Cells Derived from Human Embryonic Stem Cells for Cellularization of a Corneal Scaffold. Transl Vis Sci Technol. 2018;7:23 pubmed publisher
Zhang Q, Agoston A, Pham T, Zhang W, Zhang X, Huo X, et al. Acidic Bile Salts Induce Epithelial to Mesenchymal Transition via VEGF Signaling in Non-Neoplastic Barrett's Cells. Gastroenterology. 2019;156:130-144.e10 pubmed publisher
Brosnan Cashman J, Yuan M, Graham M, Rizzo A, Myers K, Davis C, et al. ATRX loss induces multiple hallmarks of the alternative lengthening of telomeres (ALT) phenotype in human glioma cell lines in a cell line-specific manner. PLoS ONE. 2018;13:e0204159 pubmed publisher
Hernandez C, Wang Z, Ramazanov B, Tang Y, Mehta S, Dambrot C, et al. Dppa2/4 Facilitate Epigenetic Remodeling during Reprogramming to Pluripotency. Cell Stem Cell. 2018;23:396-411.e8 pubmed publisher
Li L, Tian E, Chen X, Chao J, Klein J, Qu Q, et al. GFAP Mutations in Astrocytes Impair Oligodendrocyte Progenitor Proliferation and Myelination in an hiPSC Model of Alexander Disease. Cell Stem Cell. 2018;23:239-251.e6 pubmed publisher
Høye A, Tolstrup S, Horton E, Nicolau M, Frost H, Woo J, et al. Tumor endothelial marker 8 promotes cancer progression and metastasis. Oncotarget. 2018;9:30173-30188 pubmed publisher
Chen A, Liu A, Krishnakumar R, Freimer J, DeVeale B, Blelloch R. GRHL2-Dependent Enhancer Switching Maintains a Pluripotent Stem Cell Transcriptional Subnetwork after Exit from Naive Pluripotency. Cell Stem Cell. 2018;23:226-238.e4 pubmed publisher
Zimmermann M, Murina O, Reijns M, Agathanggelou A, Challis R, Tarnauskaitė Ž, et al. CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. Nature. 2018;559:285-289 pubmed publisher
Benitez Guijarro M, Lopez Ruiz C, Tarnauskaitė Ž, Murina O, Mian Mohammad M, Williams T, et al. RNase H2, mutated in Aicardi-Goutières syndrome, promotes LINE-1 retrotransposition. EMBO J. 2018;37: pubmed publisher
Zhang M, Di Martino J, Bowman R, Campbell N, Baksh S, Simon Vermot T, et al. Adipocyte-Derived Lipids Mediate Melanoma Progression via FATP Proteins. Cancer Discov. 2018;8:1006-1025 pubmed publisher
Sahasrabudhe N, Lenos K, van der Horst J, Rodriguez E, van Vliet S. Oncogenic BRAFV600E drives expression of MGL ligands in the colorectal cancer cell line HT29 through N-acetylgalactosamine-transferase 3. Biol Chem. 2018;399:649-659 pubmed publisher
Riesenberg S, Maricic T. Targeting repair pathways with small molecules increases precise genome editing in pluripotent stem cells. Nat Commun. 2018;9:2164 pubmed publisher
Corsini N, Peer A, Moeseneder P, Roiuk M, Burkard T, Theussl H, et al. Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation. Cell Stem Cell. 2018;22:543-558.e12 pubmed publisher
Chen J, Markelc B, Kaeppler J, Ogundipe V, Cao Y, McKenna W, et al. STING-Dependent Interferon-λ1 Induction in HT29 Cells, a Human Colorectal Cancer Cell Line, After Gamma-Radiation. Int J Radiat Oncol Biol Phys. 2018;101:97-106 pubmed publisher
Moroni M, Servin Vences M, Fleischer R, Sánchez Carranza O, Lewin G. Voltage gating of mechanosensitive PIEZO channels. Nat Commun. 2018;9:1096 pubmed publisher
Lie A Ling M, Marinopoulou E, Lilly A, Challinor M, Patel R, Lancrin C, et al. Regulation of RUNX1 dosage is crucial for efficient blood formation from hemogenic endothelium. Development. 2018;145: pubmed publisher
Yang L, Wang R, Yang S, Ma Z, Lin S, Nan Y, et al. Karyopherin Alpha 6 Is Required for Replication of Porcine Reproductive and Respiratory Syndrome Virus and Zika Virus. J Virol. 2018;92: pubmed publisher
Olley G, Ansari M, Bengani H, Grimes G, Rhodes J, von Kriegsheim A, et al. BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange-like syndrome. Nat Genet. 2018;50:329-332 pubmed publisher
Cleyrat C, Girard R, Choi E, Jeziorski E, Lavabre Bertrand T, Hermouet S, et al. Gene editing rescue of a novel MPL mutant associated with congenital amegakaryocytic thrombocytopenia. Blood Adv. 2017;1:1815-1826 pubmed publisher
Steenpass L. Generation of two H1 hESC sublines carrying a heterozygous and homozygous knock-out of RB1. Stem Cell Res. 2017;25:270-273 pubmed publisher
Adikusuma F, Pfitzner C, Thomas P. Versatile single-step-assembly CRISPR/Cas9 vectors for dual gRNA expression. PLoS ONE. 2017;12:e0187236 pubmed publisher
Vanli G, Cuesta Marbán A, Widmann C. Evaluation and validation of commercial antibodies for the detection of Shb. PLoS ONE. 2017;12:e0188311 pubmed publisher
Mitkin N, Muratova A, Sharonov G, Korneev K, Sviriaeva E, Mazurov D, et al. p63 and p73 repress CXCR5 chemokine receptor gene expression in p53-deficient MCF-7 breast cancer cells during genotoxic stress. Biochim Biophys Acta Gene Regul Mech. 2017;1860:1169-1178 pubmed publisher
Stevenson N, Bergen D, Skinner R, Kague E, Martin Silverstone E, Robson Brown K, et al. Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression. J Cell Sci. 2017;130:4132-4143 pubmed publisher
Kopp Y, Lang W, Schuster T, Martínez Limón A, Hofbauer H, Ernst R, et al. CHIP as a membrane-shuttling proteostasis sensor. elife. 2017;6: pubmed publisher
Bartosovic M, Molares H, Gregorová P, Hrossova D, Kudla G, Vanacova S. N6-methyladenosine demethylase FTO targets pre-mRNAs and regulates alternative splicing and 3'-end processing. Nucleic Acids Res. 2017;45:11356-11370 pubmed publisher
Samwer M, Schneider M, Hoefler R, Schmalhorst P, Jude J, Zuber J, et al. DNA Cross-Bridging Shapes a Single Nucleus from a Set of Mitotic Chromosomes. Cell. 2017;170:956-972.e23 pubmed publisher
Rattanasinchai C, Llewellyn B, Conrad S, Gallo K. MLK3 regulates FRA-1 and MMPs to drive invasion and transendothelial migration in triple-negative breast cancer cells. Oncogenesis. 2017;6:e345 pubmed publisher
Pettersen K, Andersen S, Degen S, Tadini V, Grosjean J, Hatakeyama S, et al. Cancer cachexia associates with a systemic autophagy-inducing activity mimicked by cancer cell-derived IL-6 trans-signaling. Sci Rep. 2017;7:2046 pubmed publisher
Ashdown G, Burn G, Williamson D, Pandžić E, Peters R, Holden M, et al. Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse. Biophys J. 2017;112:1703-1713 pubmed publisher
Liu N, ter Huurne M, Nguyen L, Peng T, Wang S, Studd J, et al. The non-coding variant rs1800734 enhances DCLK3 expression through long-range interaction and promotes colorectal cancer progression. Nat Commun. 2017;8:14418 pubmed publisher
Gao Y, Wu H, Wang Y, Liu X, Chen L, Li Q, et al. Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects. Genome Biol. 2017;18:13 pubmed publisher
Wall A, Luo L, Hung Y, Tong S, Condon N, Blumenthal A, et al. Small GTPase Rab8a-recruited Phosphatidylinositol 3-Kinase γ Regulates Signaling and Cytokine Outputs from Endosomal Toll-like Receptors. J Biol Chem. 2017;292:4411-4422 pubmed publisher
Hudson D, Amor D, Boys A, Butler K, Williams L, Zhang T, et al. Loss of RMI2 Increases Genome Instability and Causes a Bloom-Like Syndrome. PLoS Genet. 2016;12:e1006483 pubmed publisher
Shah R, Cholewa Waclaw J, Davies F, Paton K, Chaligne R, Heard E, et al. Efficient and versatile CRISPR engineering of human neurons in culture to model neurological disorders. Wellcome Open Res. 2016;1:13 pubmed publisher
Termglinchan V, Seeger T, Chen C, Wu J, Karakikes I. Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro. Methods Mol Biol. 2017;1521:55-68 pubmed
Westphal A, Cheng W, Yu J, Grassl G, Krautkrämer M, Holst O, et al. Lysosomal trafficking regulator Lyst links membrane trafficking to toll-like receptor-mediated inflammatory responses. J Exp Med. 2017;214:227-244 pubmed publisher
Martínez Limón A, Alriquet M, Lang W, Calloni G, Wittig I, Vabulas R. Recognition of enzymes lacking bound cofactor by protein quality control. Proc Natl Acad Sci U S A. 2016;113:12156-12161 pubmed
Santos D, Kiskinis E, Eggan K, Merkle F. Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Curr Protoc Stem Cell Biol. 2016;38:5B.6.1-5B.6.60 pubmed publisher
Bento C, Ashkenazi A, Jimenez Sanchez M, Rubinsztein D. The Parkinson's disease-associated genes ATP13A2 and SYT11 regulate autophagy via a common pathway. Nat Commun. 2016;7:11803 pubmed publisher
Chiapparo G, Lin X, Lescroart F, Chabab S, Paulissen C, Pitisci L, et al. Mesp1 controls the speed, polarity, and directionality of cardiovascular progenitor migration. J Cell Biol. 2016;213:463-77 pubmed publisher
Chiang T, le Sage C, Larrieu D, Demir M, Jackson S. CRISPR-Cas9(D10A) nickase-based genotypic and phenotypic screening to enhance genome editing. Sci Rep. 2016;6:24356 pubmed publisher
Vojta A, Dobrinić P, Tadić V, Bočkor L, Korać P, Julg B, et al. Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res. 2016;44:5615-28 pubmed publisher
Mackenzie K, Carroll P, Lettice L, TarnauskaitÄ— Å, Reddy K, Dix F, et al. Ribonuclease H2 mutations induce a cGAS/STING-dependent innate immune response. EMBO J. 2016;35:831-44 pubmed publisher
Nielsen C, Huttner D, Bizard A, Hirano S, Li T, Palmai Pallag T, et al. PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis. Nat Commun. 2015;6:8962 pubmed publisher
Kelly S, Georgomanolis T, Zirkel A, Diermeier S, O Reilly D, Murphy S, et al. Splicing of many human genes involves sites embedded within introns. Nucleic Acids Res. 2015;43:4721-32 pubmed publisher
Ran F, Hsu P, Wright J, Agarwala V, Scott D, Zhang F. Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013;8:2281-2308 pubmed publisher
product information
Catalog Number :
48140
Product Name :
pSpCas9n(BB)-2A-GFP (PX461)
article :
doi10.1038/nprot.2013.143
id7475
pubmed_id24157548
bacterial resistance :
Ampicillin
cloning :
backbonePX461
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/.
growth strain :
Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA
origin :
37
pi :
alt_names
SpCas9n-2A-GFP
Cas9
PX461
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoterCbh
sequencing_primer_3
sequencing_primer_5TTTATGGCGAGGCGGCGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationCas9 D10A nickase mutant
namehSpCas9n
shRNA_sequence
size5400
species
100
Synthetic
tags
locationN terminal on insert
tag3XFLAG
locationC terminal on insert
tagGFP
resistance markers :
898
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA