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product summary
company name :
Addgene
product type :
cDNA
product name :
pSpCas9(BB)-2A-Puro (PX459)
catalog :
48139
citations: 469
Reference
Chaib S, L xf3 pez Dom xed nguez J, Lalinde Guti xe9 rrez M, Prats N, Marin I, Boix O, et al. The efficacy of chemotherapy is limited by intratumoral senescent cells expressing PD-L2. Nat Cancer. 2024;: pubmed publisher
Tan Y, Chiou Y, Guo H, Zhang S, Huang X, Dukanya D, et al. Vertical pathway inhibition of receptor tyrosine kinases and BAD with synergistic efficacy in triple negative breast cancer. NPJ Precis Oncol. 2024;8:8 pubmed publisher
Sun M, Wang Y, Xin G, Yang B, Jiang Q, Zhang C. NuSAP regulates microtubule flux and Kif2A localization to ensure accurate chromosome congression. J Cell Biol. 2024;223: pubmed publisher
Wu G, Wang Q, Wang D, Xiong F, Liu W, Chen J, et al. Targeting polycomb repressor complex 2-mediated bivalent promoter epigenetic silencing of secreted frizzled-related protein 1 inhibits cholangiocarcinoma progression. Clin Transl Med. 2023;13:e1502 pubmed publisher
Muccioli S, Brillo V, Varanita T, Rossin F, Zaltron E, Velle A, et al. Transglutaminase Type 2-MITF axis regulates phenotype switching in skin cutaneous melanoma. Cell Death Dis. 2023;14:704 pubmed publisher
Limthongkul J, Akkarasereenon K, Yodweerapong T, Songthammawat P, Tong Ngam P, Tubsuwan A, et al. Novel Potent Autophagy Inhibitor Ka-003 Inhibits Dengue Virus Replication. Viruses. 2023;15: pubmed publisher
Sugahara S, Ishino Y, Sawada K, Iwata T, Shimanaka Y, Aoki J, et al. Disease-related PSS1 mutant impedes the formation and function of osteoclasts. J Lipid Res. 2023;64:100443 pubmed publisher
Nemudraia A, Nemudryi A, Wiedenheft B. Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells. bioRxiv. 2023;: pubmed publisher
Dey D, Dasgupta A, Ghosh D, Bhattacharjee O, Ghosh A, Honda A, et al. Host proteins alpha 2 macroglobulin and LRP1 associate with Chandipura virus. Biochimie. 2023;: pubmed publisher
Lee J, Kim Y, Ataliotis P, Kim H, Kim D, Bennett D, et al. Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development. Sci Rep. 2023;13:12309 pubmed publisher
Kakumani P, Ko Y, Ramakrishna S, Christopher G, Dodgson M, Shrinet J, et al. CSDE1 promotes miR-451 biogenesis. Nucleic Acids Res. 2023;: pubmed publisher
Hsu F, Wu Q, Fabyanic E, Wei A, Wu H, Clark A. TET1 facilitates specification of early human lineages including germ cells. iScience. 2023;26:107191 pubmed publisher
Sato K, Yamashita T, Ohuchi H. Mammalian type Opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response. J Biol Chem. 2023;:105020 pubmed publisher
Salem O, Jia S, Qian B, Hansen C. AR activates YAP/TAZ differentially in prostate cancer. Life Sci Alliance. 2023;6: pubmed publisher
Sindram E, Caballero Oteyza A, Kogata N, Chor Mei Huang S, Alizadeh Z, G xe1 mez D xed az L, et al. ARPC5 deficiency leads to severe early-onset systemic inflammation and mortality. Dis Model Mech. 2023;16: pubmed publisher
Pleiner T, Hazu M, Pinton Tomaleri G, Nguyen V, Januszyk K, Voorhees R. A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER. J Cell Biol. 2023;222: pubmed publisher
Nwosu Z, Ward M, Sajjakulnukit P, Poudel P, Ragulan C, Kasperek S, et al. Uridine-derived ribose fuels glucose-restricted pancreatic cancer. Nature. 2023;618:151-158 pubmed publisher
Powell A, Edwards N, Hunter H, Kiser P, Watson A, Cumming R, et al. Deletion of p66Shc Dysregulates ERK and STAT3 Activity in Embryonic Stem Cells, Enhancing Their Naive-Like Self-Renewal in the Presence of Leukemia Inhibitory Factor. Stem Cells Dev. 2023;: pubmed publisher
Chen Y, Zhou J, Wu S, Wang L, Chen G, Chen D, et al. ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1. Cell Biosci. 2023;13:84 pubmed publisher
Fu Y, Zi R, Xiong S. Infection by exosome-carried Coxsackievirus B3 induces immune escape resulting in an aggravated pathogenesis. Microbes Infect. 2023;:105148 pubmed publisher
Yang L, Li H, Han Y, Song Y, Wei M, Fang M, et al. CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation. Genes (Basel). 2023;14: pubmed publisher
Malik N, Ferreira B, Hollstein P, Curtis S, Trefts E, Weiser Novak S, et al. Induction of lysosomal and mitochondrial biogenesis by AMPK phosphorylation of FNIP1. Science. 2023;380:eabj5559 pubmed publisher
Du Q, Stow E, LaCoste D, Freeman B, Baddoo M, Shareef A, et al. A novel role of TRIM28 B box domain in L1 retrotransposition and ORF2p-mediated cDNA synthesis. Nucleic Acids Res. 2023;51:4429-4450 pubmed publisher
Kurhade C, Kang S, Biering S, Hwang S, Randall G. CAPRIN1 Is Required for Control of Viral Replication Complexes by Interferon Gamma. MBio. 2023;14:e0017223 pubmed publisher
Carnie C, Armstrong L, Sebesta M, Ariza A, Wang X, Graham E, et al. ERCC6L2 mitigates replication stress and promotes centromere stability. Cell Rep. 2023;42:112329 pubmed publisher
Park W, Gray J, Holewinski R, Andresson T, So J, Carmona Rivera C, et al. Apoptosis-induced nuclear expulsion in tumor cells drives S100a4-mediated metastatic outgrowth through the RAGE pathway. Nat Cancer. 2023;4:419-435 pubmed publisher
Gao S, Zhang W, Ma J, Ni X. PHF6 recruits BPTF to promote HIF-dependent pathway and progression in YAP-high breast cancer. J Transl Med. 2023;21:220 pubmed publisher
Das S, Gupta V, Bjorge J, Shi X, Gong W, Garry M, et al. ETV2 and VEZF1 interaction and regulation of the hematoendothelial lineage during embryogenesis. Front Cell Dev Biol. 2023;11:1109648 pubmed publisher
Hu L, Zhao C, Liu M, Liu S, Ye J, Wang K, et al. CENP-I directly targets centromeric DNA to support CENP-A deposition and centromere maintenance. Proc Natl Acad Sci U S A. 2023;120:e2219170120 pubmed publisher
Hall M, Shajahan A, Burch A, Hatchett C, Azadi P, Schwalbe R. Limited N-Glycan Processing Impacts Chaperone Expression Patterns, Cell Growth and Cell Invasiveness in Neuroblastoma. Biology (Basel). 2023;12: pubmed publisher
Vanoli F, Herviou L, Tsuda Y, Sung P, Xie Z, Fishinevich E, et al. Generating in vitro models of NTRK-fusion mesenchymal neoplasia as tools for investigating kinase oncogenic activation and response to targeted therapy. Oncogenesis. 2023;12:8 pubmed publisher
Bhardwaj M, Lee J, Versace A, Harper S, Goldman A, Crissey M, et al. Lysosomal lipid peroxidation regulates tumor immunity. J Clin Invest. 2023;133: pubmed publisher
Xie W, Han Z, Zuo Z, Xin D, Chen H, Huang J, et al. ASAP1 activates the IQGAP1/CDC42 pathway to promote tumor progression and chemotherapy resistance in gastric cancer. Cell Death Dis. 2023;14:124 pubmed publisher
Nakashima S, Matsui T, Fukuda M. Vps9d1 regulates tubular endosome formation through specific activation of Rab22A. J Cell Sci. 2023;136: pubmed publisher
Cunningham R, Jia S, Purohit K, Salem O, Hui N, Lin Y, et al. YAP/TAZ activation predicts clinical outcomes in mesothelioma and is conserved in in vitro model of driver mutations. Clin Transl Med. 2023;13:e1190 pubmed publisher
Lv J, Zhou Y, Zhou N, Wang Z, Chen J, Chen H, et al. Epigenetic modification of CSDE1 locus dictates immune recognition of nascent tumorigenic cells. Sci Transl Med. 2023;15:eabq6024 pubmed publisher
F xe4 xdf ler F, Javoor M, Datler J, D xf6 ring H, Hofer F, Dimchev G, et al. ArpC5 isoforms regulate Arp2/3 complex-dependent protrusion through differential Ena/VASP positioning. Sci Adv. 2023;9:eadd6495 pubmed publisher
Arimoto K, Miyauchi S, Troutman T, Zhang Y, Liu M, Stoner S, et al. Expansion of interferon inducible gene pool via USP18 inhibition promotes cancer cell pyroptosis. Nat Commun. 2023;14:251 pubmed publisher
Chen K, Reinshagen C, Van Schaik T, Rossignoli F, Borges P, Mendonca N, et al. Bifunctional cancer cell-based vaccine concomitantly drives direct tumor killing and antitumor immunity. Sci Transl Med. 2023;15:eabo4778 pubmed publisher
Saponaro M, Flottmann S, Eckstein M, Hommerding O, Kl xfc mper N, Corvino D, et al. CDCP1 expression is frequently increased in aggressive urothelial carcinoma and promotes urothelial tumor progression. Sci Rep. 2023;13:73 pubmed publisher
Senkevich K, Zorca C, Dworkind A, Rudakou U, Somerville E, Yu E, et al. GALC variants affect galactosylceramidase enzymatic activity and risk of Parkinson's disease. Brain. 2023;146:1859-1872 pubmed publisher
Gu X, Dai Q, Du P, Li N, Li J, Zeng S, et al. Pold4 is dispensable for mouse development, DNA replication and DNA repair. Gene. 2023;851:147029 pubmed publisher
Yamashita G, Takano N, Kazama H, Tsukahara K, Miyazawa K. p53 regulates lysosomal membrane permeabilization as well as cytoprotective autophagy in response to DNA-damaging drugs. Cell Death Discov. 2022;8:502 pubmed publisher
Griess K, Rieck M, M xfc ller N, Karsai G, Hartwig S, Pelligra A, et al. Sphingolipid subtypes differentially control proinsulin processing and systemic glucose homeostasis. Nat Cell Biol. 2022;: pubmed publisher
Ozawa M, Taguchi J, Katsuma K, Ishikawa Yamauchi Y, Kikuchi M, Sakamoto R, et al. Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice. Sci Rep. 2022;12:21558 pubmed publisher
Guo D, Merrill R, Qian L, Hsu Y, Zhang Q, Lin Z, et al. The BBSome regulates mitochondria dynamics and function. Mol Metab. 2022;67:101654 pubmed publisher
Karpov D, Demidova N, Kulagin K, Shuvalova A, Kovalev M, Simonov R, et al. Complete and Prolonged Inhibition of Herpes Simplex Virus Type 1 Infection In Vitro by CRISPR/Cas9 and CRISPR/CasX Systems. Int J Mol Sci. 2022;23: pubmed publisher
Weber R, Kleemann L, Hirschberg I, Chung M, Valkov E, Igreja C. DAP5 enables main ORF translation on mRNAs with structured and uORF-containing 5' leaders. Nat Commun. 2022;13:7510 pubmed publisher
Aylon Y, Furth N, Mallel G, Friedlander G, Nataraj N, Dong M, et al. Breast cancer plasticity is restricted by a LATS1-NCOR1 repressive axis. Nat Commun. 2022;13:7199 pubmed publisher
Akerberg A, Trembley M, Butty V, Schwertner A, Zhao L, Beerens M, et al. RBPMS2 Is a Myocardial-Enriched Splicing Regulator Required for Cardiac Function. Circ Res. 2022;131:980-1000 pubmed publisher
Kang K, Shi Q, Wang X, Chen Y. Dishevelled phase separation promotes Wnt signalosome assembly and destruction complex disassembly. J Cell Biol. 2022;221: pubmed publisher
Yang Y, Cao L, Guo Z, Gu H, Zhang K, Qiu Z. Deubiquitinase UCHL5 stabilizes ELK3 to potentiate cancer stemness and tumor progression in pancreatic adenocarcinoma (PAAD). Exp Cell Res. 2022;421:113402 pubmed publisher
Mahen R. cNap1 bridges centriole contact sites to maintain centrosome cohesion. PLoS Biol. 2022;20:e3001854 pubmed publisher
Zhou M, Huang P, Bai R, Liu X. Generation of a homozygous TAZ knockout hESCs line by CRISPR/Cas9 system. Stem Cell Res. 2022;64:102923 pubmed publisher
Hino N, Matsuda K, Jikko Y, Maryu G, Sakai K, Imamura R, et al. A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. Dev Cell. 2022;57:2290-2304.e7 pubmed publisher
Yoon S, Lee S, Park C, Choi H, Yoo M, Lee S, et al. An Efficacious Transgenic Strategy for Triple Knockout of Xeno-Reactive Antigen Genes GGTA1, CMAH, and B4GALNT2 from Jeju Native Pigs. Vaccines (Basel). 2022;10: pubmed publisher
Zhang W, Yang X, Li Y, Yu L, Zhang B, Zhang J, et al. GCAF(TMEM251) regulates lysosome biogenesis by activating the mannose-6-phosphate pathway. Nat Commun. 2022;13:5351 pubmed publisher
Tsujimoto G, Ito R, Yoshikawa K, Ueki C, Okada N. NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression. Front Cell Dev Biol. 2022;10:983599 pubmed publisher
Ohshima T, Yamamoto H, Sakamaki Y, Saito C, Mizushima N. NCOA4 drives ferritin phase separation to facilitate macroferritinophagy and microferritinophagy. J Cell Biol. 2022;221: pubmed publisher
Averbeck N, Barent C, Jakob B, Syzonenko T, Durante M, Taucher Scholz G. The Ubiquitin Ligase RNF138 Cooperates with CtIP to Stimulate Resection of Complex DNA Double-Strand Breaks in Human G1-Phase Cells. Cells. 2022;11: pubmed publisher
Volkmar N, Gawden Bone C, Williamson J, Nixon Abell J, West J, St George Hyslop P, et al. Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2. EMBO J. 2022;41:e110777 pubmed publisher
Dubois A, Vincenti L, Chervova A, Greenberg M, Vandormael Pournin S, Bourc his D, et al. H3K9 tri-methylation at Nanog times differentiation commitment and enables the acquisition of primitive endoderm fate. Development. 2022;149: pubmed publisher
Masud S, Chandrashekhar M, Aregger M, Tan G, Zhang X, Mero P, et al. Chemical genomics with pyrvinium identifies C1orf115 as a regulator of drug efflux. Nat Chem Biol. 2022;18:1370-1379 pubmed publisher
Huang K, Jia Z, Li H, Peng Y, Chen X, Luo N, et al. Proto-oncogene FAM83A contributes to casein kinase 1-mediated mitochondrial maintenance and white adipocyte differentiation. J Biol Chem. 2022;298:102339 pubmed publisher
Millrine D, Cummings T, Matthews S, Peter J, Magnussen H, Lange S, et al. Human UFSP1 is an active protease that regulates UFM1 maturation and UFMylation. Cell Rep. 2022;40:111168 pubmed publisher
Tsukamoto Y, Kurogi S, Shibata T, Suzuki K, Hirashita Y, Fumoto S, et al. Enhanced phosphorylation of c-Jun by cisplatin treatment as a potential predictive biomarker for cisplatin response in combination with patient-derived tumor organoids. Lab Invest. 2022;102:1355-1366 pubmed publisher
Zhao C, Gong J, Bai Y, Yin T, Zhou M, Pan S, et al. A self-amplifying USP14-TAZ loop drives the progression and liver metastasis of pancreatic ductal adenocarcinoma. Cell Death Differ. 2022;: pubmed publisher
Quijano Rubio C, Silginer M, Weller M. CD95 gene deletion may reduce clonogenic growth and invasiveness of human glioblastoma cells in a CD95 ligand-independent manner. Cell Death Discov. 2022;8:341 pubmed publisher
Zuo Z, Liu J, Sun Z, Silverstein R, Zou M, Finkel T, et al. A potent tumor-selective ERK pathway inactivator with high therapeutic index. PNAS Nexus. 2022;1:pgac104 pubmed publisher
Park J, Jia S, Salter D, Bagnaninchi P, Hansen C. The Hippo pathway drives the cellular response to hydrostatic pressure. EMBO J. 2022;41:e108719 pubmed publisher
Wang Y, Liu X, Obser T, Bauer A, Heyes M, Starzonek S, et al. Heparan sulfate dependent binding of plasmatic von Willebrand factor to blood circulating melanoma cells attenuates metastasis. Matrix Biol. 2022;: pubmed publisher
Zhang Y, Fang Y, Tang Y, Han S, Jia J, Wan X, et al. SMYD5 catalyzes histone H3 lysine 36 trimethylation at promoters. Nat Commun. 2022;13:3190 pubmed publisher
Okumura W, Kozono T, Sato H, Matsui H, Takagi T, Tonozuka T, et al. Jaw1/LRMP increases Ca2+ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype. Sci Rep. 2022;12:9476 pubmed publisher
Somuncu B, Ekmekcioglu A, Antmen F, Ertuzun T, Deniz E, Keskin N, et al. Targeting mitochondrial DNA polymerase gamma for selective inhibition of MLH1 deficient colon cancer growth. PLoS ONE. 2022;17:e0268391 pubmed publisher
Liu H, Bai Y, Zhang X, Gao T, Liu Y, Li E, et al. SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication. J Virol. 2022;96:e0041222 pubmed publisher
Severino J, Bauer M, Mattimoe T, Arecco N, Cozzuto L, Lorden P, et al. Controlled X-chromosome dynamics defines meiotic potential of female mouse in vitro germ cells. EMBO J. 2022;41:e109457 pubmed publisher
Schembs L, Willems A, Hasenpusch Theil K, Cooper J, Whiting K, Burr K, et al. The ciliary gene INPP5E confers dorsal telencephalic identity to human cortical organoids by negatively regulating Sonic hedgehog signaling. Cell Rep. 2022;39:110811 pubmed publisher
Liu Y, Deguchi Y, Wei D, Liu F, Moussalli M, Deguchi E, et al. Rapid acceleration of KRAS-mutant pancreatic carcinogenesis via remodeling of tumor immune microenvironment by PPARδ. Nat Commun. 2022;13:2665 pubmed publisher
Papes F, Camargo A, de Souza J, Carvalho V, Szeto R, LaMontagne E, et al. Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content. Nat Commun. 2022;13:2387 pubmed publisher
Zhu L, Gao T, Huang Y, Jin J, Wang D, Zhang L, et al. Ebola virus VP35 hijacks the PKA-CREB1 pathway for replication and pathogenesis by AKIP1 association. Nat Commun. 2022;13:2256 pubmed publisher
Hashemabadi M, Sasan H, Amandadi M, Esmaeilzadeh Salestani K, Esmaeili Mahani S, Ravan H. CRISPR/Cas9-Mediated Disruption of ZNF543 Gene: An Approach Toward Discovering Its Relation to TRIM28 Gene in Parkinson's Disease. Mol Biotechnol. 2022;: pubmed publisher
Zhang T, Zheng R, Li M, Yan C, Lan X, Tong B, et al. Active endogenous retroviral elements in human pluripotent stem cells play a role in regulating host gene expression. Nucleic Acids Res. 2022;50:4959-4973 pubmed publisher
Cao K, Riley J, Heilig R, Montes Gómez A, Vringer E, Berthenet K, et al. Mitochondrial dynamics regulate genome stability via control of caspase-dependent DNA damage. Dev Cell. 2022;57:1211-1225.e6 pubmed publisher
Solvik T, Nguyen T, Tony Lin Y, Marsh T, Huang E, Wiita A, et al. Secretory autophagy maintains proteostasis upon lysosome inhibition. J Cell Biol. 2022;221: pubmed publisher
Kage F, Vicente Manzanares M, McEwan B, Kettenbach A, Higgs H. Myosin II proteins are required for organization of calcium-induced actin networks upstream of mitochondrial division. Mol Biol Cell. 2022;33:ar63 pubmed publisher
Lu G, Tan H, Schmauck Medina T, Wang L, Chen J, Cho Y, et al. WIPI2 positively regulates mitophagy by promoting mitochondrial recruitment of VCP. Autophagy. 2022;:1-15 pubmed publisher
Mizuno K, Izumi T. Munc13b stimulus-dependently accumulates on granuphilin-mediated, docked granules prior to fusion. Cell Struct Funct. 2022;47:31-41 pubmed publisher
Duy P, Weise S, Marini C, Li X, Liang D, Dahl P, et al. Impaired neurogenesis alters brain biomechanics in a neuroprogenitor-based genetic subtype of congenital hydrocephalus. Nat Neurosci. 2022;25:458-473 pubmed publisher
Miyake K, Takano N, Kazama H, Kikuchi H, Hiramoto M, Tsukahara K, et al. Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells. Int J Oncol. 2022;60: pubmed publisher
Peters F, Ebner L, Atac D, Maggi J, Berger W, den Hollander A, et al. Regulation of ABCA1 by AMD-Associated Genetic Variants and Hypoxia in iPSC-RPE. Int J Mol Sci. 2022;23: pubmed publisher
Fung T, Chakrabarti R, Kollasser J, Rottner K, Stradal T, Kage F, et al. Parallel kinase pathways stimulate actin polymerization at depolarized mitochondria. Curr Biol. 2022;32:1577-1592.e8 pubmed publisher
Xu M, Zhao Y, Zhang W, Geng M, Liu Q, Gao Q, et al. Genome-scale screening in a rat haploid system identifies Thop1 as a modulator of pluripotency exit. Cell Prolif. 2022;55:e13209 pubmed publisher
Lee D, Apelt K, Lee S, Chan H, Luijsterburg M, Leung J, et al. ZMYM2 restricts 53BP1 at DNA double-strand breaks to favor BRCA1 loading and homologous recombination. Nucleic Acids Res. 2022;50:3922-3943 pubmed publisher
Bollen Y, Hageman J, van Leenen P, Derks L, Ponsioen B, Buissant des Amorie J, et al. Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage. PLoS Biol. 2022;20:e3001527 pubmed publisher
Watanabe A, Maeda K, Nara A, Hashida M, Ozono M, Nakao A, et al. Quantitative analysis of mitochondrial calcium uniporter (MCU) and essential MCU regulator (EMRE) in mitochondria from mouse tissues and HeLa cells. FEBS Open Bio. 2022;12:811-826 pubmed publisher
Remy J, Linder B, Weirauch U, Day B, Stringer B, Herold Mende C, et al. STAT3 Enhances Sensitivity of Glioblastoma to Drug-Induced Autophagy-Dependent Cell Death. Cancers (Basel). 2022;14: pubmed publisher
Alerasool N, Leng H, Lin Z, Gingras A, Taipale M. Identification and functional characterization of transcriptional activators in human cells. Mol Cell. 2022;82:677-695.e7 pubmed publisher
Uceda Castro R, van Asperen J, Vennin C, Sluijs J, van Bodegraven E, Margarido A, et al. GFAP splice variants fine-tune glioma cell invasion and tumour dynamics by modulating migration persistence. Sci Rep. 2022;12:424 pubmed publisher
Seronick E, SON J, Michael C, Fogg H, Gromley Z, Gromley A. CRISPR/Cas9 genome editing system confirms centriolin's role in cytokinesis. BMC Res Notes. 2022;15:8 pubmed publisher
Song X, Cui Y, Wang Y, Zhang Y, He Q, Yu Z, et al. Genome Editing with AAV-BR1-CRISPR in Postnatal Mouse Brain Endothelial Cells. Int J Biol Sci. 2022;18:652-660 pubmed publisher
Wei R, Yang J, Cheng C, Ho W, Li N, Hu Y, et al. CRISPR-targeted genome editing of human induced pluripotent stem cell-derived hepatocytes for the treatment of Wilson's disease. JHEP Rep. 2022;4:100389 pubmed publisher
Feng J, Li Y, Hu Y, Zhou Y, Zhang H, Wu F. Novel Quinic Acid Glycerates from Tussilago farfara Inhibit Polypeptide GalNAc-Transferase. Chembiochem. 2022;23:e202100539 pubmed publisher
Cortizo F, Pfaff D, Wirth A, Schlotterer A, Medert R, Morgenstern J, et al. The activity of glyoxylase 1 is regulated by glucose-responsive phosphorylation on Tyr136. Mol Metab. 2022;55:101406 pubmed publisher
Miyashita Y, Kouwaki T, Tsukamoto H, Okamoto M, Nakamura K, Oshiumi H. TICAM-1/TRIF associates with Act1 and suppresses IL-17 receptor-mediated inflammatory responses. Life Sci Alliance. 2022;5: pubmed publisher
Deng C, Hu F, Zhao Z, Zhou Y, Liu Y, Zhang T, et al. The Establishment of Quantitatively Regulating Expression Cassette with sgRNA Targeting BIRC5 to Elucidate the Synergistic Pathway of Survivin with P-Glycoprotein in Cancer Multi-Drug Resistance. Front Cell Dev Biol. 2021;9:797005 pubmed publisher
Strohmeier K, Hofmann M, Hauser F, Sivun D, Puthukodan S, Karner A, et al. CRISPR/Cas9 Genome Editing vs. Over-Expression for Fluorescent Extracellular Vesicle-Labeling: A Quantitative Analysis. Int J Mol Sci. 2021;23: pubmed publisher
Lam D, Williams R, Lujan E, Tanabe K, Huber G, Saw N, et al. Collagen VI regulates motor circuit plasticity and motor performance by cannabinoid modulation. J Neurosci. 2021;: pubmed publisher
Ito N, Takahashi T, Shiiba I, Nagashima S, Inatome R, Yanagi S. MITOL regulates phosphatidic acid-binding activity of RMDN3/PTPIP51. J Biochem. 2021;: pubmed publisher
Ikami Y, Terasawa K, Sakamoto K, Ohtake K, Harada H, Watabe T, et al. The two-domain architecture of LAMP2A regulates its interaction with Hsc70. Exp Cell Res. 2021;411:112986 pubmed publisher
Hirayama H, Tachida Y, Seino J, Suzuki T. A method for assaying peptide: N-glycanase/N-Glycanase 1 activities in crude extracts using an N-glycosylated cyclopeptide. Glycobiology. 2021;: pubmed publisher
Zhou Q, Wang K, Qiu J, Zhu D, Tian T, Zhang Y, et al. Comparative transcriptome analysis and CRISPR/Cas9 gene editing reveal that E4BP4 mediates lithium upregulation of Per2 expression. Open Biol. 2021;11:210140 pubmed publisher
Jacobs Catane L, Moshel O, Smith Y, Davidson B, Reich R. Splice-Variant Knock-Out of TGFβ Receptors Perturbates the Proteome of Ovarian Carcinoma Cells. Int J Mol Sci. 2021;22: pubmed publisher
Dong X, Zhang Q, Hao J, Xie Q, Xu B, Zhang P, et al. Large Multicohort Study Reveals a Prostate Cancer Susceptibility Allele at 5p15 Regulating TERT via Androgen Signaling-Orchestrated Chromatin Binding of E2F1 and MYC. Front Oncol. 2021;11:754206 pubmed publisher
Podinovskaia M, Prescianotto Baschong C, Buser D, Spang A. A novel live-cell imaging assay reveals regulation of endosome maturation. elife. 2021;10: pubmed publisher
Zhang Z, Han Z, Guo Y, Liu X, Gao Y, Zhang Y. Establishment of an Efficient Immortalization Strategy Using HMEJ-Based bTERT Insertion for Bovine Cells. Int J Mol Sci. 2021;22: pubmed publisher
Wu Y, Tian T, Wu Y, Yang Y, Zhang Y, Qin X. Systematic Studies of the Circadian Clock Genes Impact on Temperature Compensation and Cell Proliferation Using CRISPR Tools. Biology (Basel). 2021;10: pubmed publisher
Eghbalsaied S, Kues W. An electrochemical protocol for CRISPR-mediated gene-editing of sheep embryonic fibroblast cells. Cells Tissues Organs. 2021;: pubmed publisher
Schifferl D, Scholze Wittler M, Wittler L, Veenvliet J, Koch F, Herrmann B. A 37 kb region upstream of brachyury comprising a notochord enhancer is essential for notochord and tail development. Development. 2021;148: pubmed publisher
Yu R, Jin S, Ankarcrona M, Lendahl U, Nister M, Zhao J. The Molecular Assembly State of Drp1 Controls its Association With the Mitochondrial Recruitment Receptors Mff and MIEF1/2. Front Cell Dev Biol. 2021;9:706687 pubmed publisher
Yang D, Wu X, Wang W, Zhou Y, Wang Z. Ciliary Type III Adenylyl Cyclase in the VMH Is Crucial for High-Fat Diet-Induced Obesity Mediated by Autophagy. Adv Sci (Weinh). 2021;:e2102568 pubmed publisher
Scott W, Dhanji E, Dyakov B, Dreseris E, Asa J, Grange L, et al. ATRX proximal protein associations boast roles beyond histone deposition. PLoS Genet. 2021;17:e1009909 pubmed publisher
Hall M, Burch A, Schwalbe R. Functional analysis of N-acetylglucosaminyltransferase-I knockdown in 2D and 3D neuroblastoma cell cultures. PLoS ONE. 2021;16:e0259743 pubmed publisher
Mehidi A, Kage F, Karataş Z, Cercy M, Schaks M, Polesskaya A, et al. Forces generated by lamellipodial actin filament elongation regulate the WAVE complex during cell migration. Nat Cell Biol. 2021;23:1148-1162 pubmed publisher
McGuire M, Mukhopadhyay D, Myers S, Mosher E, Brookheart R, Kammers K, et al. Progesterone receptor membrane component 1 (PGRMC1) binds and stabilizes cytochromes P450 through a heme-independent mechanism. J Biol Chem. 2021;297:101316 pubmed publisher
Thery F, Martina L, Asselman C, Zhang Y, Vessely M, Repo H, et al. Ring finger protein 213 assembles into a sensor for ISGylated proteins with antimicrobial activity. Nat Commun. 2021;12:5772 pubmed publisher
Spek C, Aberson H, Butler J, de Vos A, Duitman J. CEBPD Potentiates the Macrophage Inflammatory Response but CEBPD Knock-Out Macrophages Fail to Identify CEBPD-Dependent Pro-Inflammatory Transcriptional Programs. Cells. 2021;10: pubmed publisher
Hall I, Climent M, Viviani Anselmi C, Papa L, Tragante V, Lambroia L, et al. rs41291957 controls miR-143 and miR-145 expression and impacts coronary artery disease risk. EMBO Mol Med. 2021;13:e14060 pubmed publisher
Liu J, Lin Y, Leidal A, Huang H, Ye J, Wiita A, et al. GRASP55 restricts early-stage autophagy and regulates spatial organization of the early secretory network. Biol Open. 2021;10: pubmed publisher
Mayca Pozo F, Geng X, Tamagno I, Jackson M, Heimsath E, Hammer J, et al. MYO10 drives genomic instability and inflammation in cancer. Sci Adv. 2021;7:eabg6908 pubmed publisher
Miyazaki M, Hiramoto M, Takano N, Kokuba H, Takemura J, Tokuhisa M, et al. Targeted disruption of GAK stagnates autophagic flux by disturbing lysosomal dynamics. Int J Mol Med. 2021;48: pubmed publisher
Yan B, Li T, Coyaud E, Laurent E, St Germain J, Zhou Y, et al. C5orf51 is a component of the MON1-CCZ1 complex and controls RAB7A localization and stability during mitophagy. Autophagy. 2021;:1-12 pubmed publisher
Kannangara A, Poole D, McEwan C, Youngs J, Weerasekara V, Thornock A, et al. BioID reveals an ATG9A interaction with ATG13-ATG101 in the degradation of p62/SQSTM1-ubiquitin clusters. EMBO Rep. 2021;:e51136 pubmed publisher
Li Y, Long W, Gao M, Jiao F, Chen Z, Liu M, et al. TREM2 Regulates High Glucose-Induced Microglial Inflammation via the NLRP3 Signaling Pathway. Brain Sci. 2021;11: pubmed publisher
Ma S, Xu Q, Bai R, Dong T, Peng Z, Liu X. Generation of a TPM1 homozygous knockout embryonic stem cell line by CRISPR/Cas9 editing. Stem Cell Res. 2021;55:102470 pubmed publisher
Garay J, Smith R, Devlin K, Hollern D, Liby T, Liu M, et al. Sensitivity to targeted therapy differs between HER2-amplified breast cancer cells harboring kinase and helical domain mutations in PIK3CA. Breast Cancer Res. 2021;23:81 pubmed publisher
Shi R, Shi X, Qin D, Tang S, Vermeulen M, Zhang X. SNX27-driven membrane localisation of OTULIN antagonises linear ubiquitination and NF-κB signalling activation. Cell Biosci. 2021;11:146 pubmed publisher
Higo S, Hikoso S, Miyagawa S, Sakata Y. Genome Editing in Human Induced Pluripotent Stem Cells (hiPSCs). Methods Mol Biol. 2021;2320:235-245 pubmed publisher
Chadha A, Paniagua A, Williams D. Comparison of ciliary targeting of two rhodopsin-like GPCRs: role of C-terminal localization sequences in relation to cilium type. J Neurosci. 2021;: pubmed publisher
Kaplun D, Starshin A, Sharko F, Gainova K, Filonova G, Zhigalova N, et al. Kaiso Regulates DNA Methylation Homeostasis. Int J Mol Sci. 2021;22: pubmed publisher
Zhang W, Yang X, Chen L, Liu Y, Venkatarangan V, Reist L, et al. A  conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation. PLoS Biol. 2021;19:e3001361 pubmed publisher
Falcomatà C, Bärthel S, Ulrich A, Diersch S, Veltkamp C, Rad L, et al. Genetic screens identify a context-specific PI3K/p27Kip1 node driving extrahepatic biliary cancer. Cancer Discov. 2021;: pubmed publisher
Chrysostomou S, Roy R, Prischi F, Thamlikitkul L, Chapman K, Mufti U, et al. Repurposed floxacins targeting RSK4 prevent chemoresistance and metastasis in lung and bladder cancer. Sci Transl Med. 2021;13: pubmed publisher
Pratto F, Brick K, Cheng G, Lam K, Cloutier J, Dahiya D, et al. Meiotic recombination mirrors patterns of germline replication in mice and humans. Cell. 2021;184:4251-4267.e20 pubmed publisher
Ruiz E, Lan L, Diefenbacher M, Riising E, Da Costa C, Chakraborty A, et al. JunD, not c-Jun, is the AP-1 transcription factor required for Ras-induced lung cancer. JCI Insight. 2021;6: pubmed publisher
Serifi I, Besta S, Karetsou Z, Giardoglou P, Beis D, Niewiadomski P, et al. Targeting of SET/I2PP2A oncoprotein inhibits Gli1 transcription revealing a new modulator of Hedgehog signaling. Sci Rep. 2021;11:13940 pubmed publisher
Dunning K, Martz A, Peralta F, Cevoli F, Boué Grabot E, Compan V, et al. P2X7 Receptors and TMEM16 Channels Are Functionally Coupled with Implications for Macropore Formation and Current Facilitation. Int J Mol Sci. 2021;22: pubmed publisher
Vanoli F, Meskauskaite B, Herviou L, Mallen W, Sung Y, Fujisawa Y, et al. Generation of human embryonic stem cell models to exploit the EWSR1-CREB fusion promiscuity as a common pathway of transformation in human tumors. Oncogene. 2021;40:5095-5104 pubmed publisher
Arlt B, Mastrobuoni G, Wuenschel J, Astrahantseff K, Eggert A, Kempa S, et al. Inhibiting PHGDH with NCT-503 reroutes glucose-derived carbons into the TCA cycle, independently of its on-target effect. J Enzyme Inhib Med Chem. 2021;36:1282-1289 pubmed publisher
Rutten M, Derks T, Huijkman N, Bos T, Kloosterhuis N, van de Kolk K, et al. Modeling Phenotypic Heterogeneity of Glycogen Storage Disease Type 1a Liver Disease in Mice by Somatic CRISPR/CRISPR-associated protein 9-Mediated Gene Editing. Hepatology. 2021;74:2491-2507 pubmed publisher
Xu P, Chang J, Zhou X, Wang W, Bamkole M, Wong E, et al. GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer's disease. J Exp Med. 2021;218: pubmed publisher
Martens Y, Xu S, Tait R, Li G, Zhao X, Lu W, et al. Generation and validation of APOE knockout human iPSC-derived cerebral organoids. STAR Protoc. 2021;2:100571 pubmed publisher
Das S, Chadwick B. CRISPR mediated targeting of DUX4 distal regulatory element represses DUX4 target genes dysregulated in Facioscapulohumeral muscular dystrophy. Sci Rep. 2021;11:12598 pubmed publisher
Huang P, Wen M, Xie X, Xu A, Lee D, Chen T. Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1. Stem Cell Res. 2021;54:102415 pubmed publisher
May H, Jeong J, Revah Politi A, Cohen J, Chassevent A, Baptista J, et al. Truncating variants in the SHANK1 gene are associated with a spectrum of neurodevelopmental disorders. Genet Med. 2021;: pubmed publisher
Xiong F, Wang R, Lee J, Li S, Chen S, Liao Z, et al. RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability. Cell Res. 2021;31:861-885 pubmed publisher
Bauer M, Vidal E, Zorita E, Üresin N, Pinter S, Filion G, et al. Chromosome compartments on the inactive X guide TAD formation independently of transcription during X-reactivation. Nat Commun. 2021;12:3499 pubmed publisher
Liu S, Mok B, Deng S, Liu H, Wang P, Song W, et al. Mammalian cells use the autophagy process to restrict avian influenza virus replication. Cell Rep. 2021;35:109213 pubmed publisher
Fang Y, Tang Y, Zhang Y, Pan Y, Jia J, Sun Z, et al. The H3K36me2 methyltransferase NSD1 modulates H3K27ac at active enhancers to safeguard gene expression. Nucleic Acids Res. 2021;49:6281-6295 pubmed publisher
Banning A, Zakrzewicz A, Chen X, Gray S, Tikkanen R. Knockout of the CMP-Sialic Acid Transporter SLC35A1 in Human Cell Lines Increases Transduction Efficiency of Adeno-Associated Virus 9: Implications for Gene Therapy Potency Assays. Cells. 2021;10: pubmed publisher
Mimura K, Sakamaki J, Morishita H, Kawazu M, Mano H, Mizushima N. Genome-wide CRISPR screening reveals nucleotide synthesis negatively regulates autophagy. J Biol Chem. 2021;296:100780 pubmed publisher
Yasutake H, Lee J, Hashimoto A, Masuyama K, Li J, Kuramoto Y, et al. Decreased YAP activity reduces proliferative ability in human induced pluripotent stem cell of duchenne muscular dystrophy derived cardiomyocytes. Sci Rep. 2021;11:10351 pubmed publisher
Kook S, Wang P, Meng S, Jetter C, Sucre J, Benjamin J, et al. AP-3-dependent targeting of flippase ATP8A1 to lamellar bodies suppresses activation of YAP in alveolar epithelial type 2 cells. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Feng D, Wang J, Yang W, Li J, Lin X, Zha F, et al. Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7-mediated Vangl ubiquitination and endoplasmic reticulum-associated degradation. Sci Adv. 2021;7: pubmed publisher
Montavon T, Baldaccini M, Lefevre M, Girardi E, Chane Woon Ming B, Messmer M, et al. Human DICER helicase domain recruits PKR and modulates its antiviral activity. PLoS Pathog. 2021;17:e1009549 pubmed publisher
Marx N, Dhiman H, Schmieder V, Freire C, Nguyen L, Klanert G, et al. Enhanced targeted DNA methylation of the CMV and endogenous promoters with dCas9-DNMT3A3L entails distinct subsequent histone modification changes in CHO cells. Metab Eng. 2021;66:268-282 pubmed publisher
Pleiner T, Hazu M, Tomaleri G, Januszyk K, Oania R, Sweredoski M, et al. WNK1 is an assembly factor for the human ER membrane protein complex. Mol Cell. 2021;81:2693-2704.e12 pubmed publisher
Li G, Kryczek I, Nam J, Li X, Li S, Li J, et al. LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy. Nat Cell Biol. 2021;23:526-537 pubmed publisher
Piette B, Alerasool N, Lin Z, Lacoste J, Lam M, Qian W, et al. Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains. Mol Cell. 2021;81:2549-2565.e8 pubmed publisher
Wang G, Niu X, Liu H, Dong Q, Yao Y, Wang D, et al. c-Abl kinase regulates cell proliferation and ionizing radiation-induced G2/M arrest via phosphorylation of FHL2. FEBS Open Bio. 2021;11:1731-1738 pubmed publisher
Almozyan S, Coulton J, Babaei Jadidi R, Nateri A. FLYWCH1, a Multi-Functional Zinc Finger Protein Contributes to the DNA Repair Pathway. Cells. 2021;10: pubmed publisher
Wang H, Gong P, Chen T, Gao S, Wu Z, Wang X, et al. Colorectal Cancer Stem Cell States Uncovered by Simultaneous Single-Cell Analysis of Transcriptome and Telomeres. Adv Sci (Weinh). 2021;8:2004320 pubmed publisher
Gao P, Dong X, Wang Y, Wei G. Optimized CRISPR/Cas9-mediated single nucleotide mutation in adherent cancer cell lines. STAR Protoc. 2021;2:100419 pubmed publisher
Hamar J, Kültz D. An efficient vector-based CRISPR/Cas9 system in an Oreochromis mossambicus cell line using endogenous promoters. Sci Rep. 2021;11:7854 pubmed publisher
Tang X, Fu J, Chen Z, Luo L, Li D, Liu Y. Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the MERTK locus. Stem Cell Res. 2021;53:102310 pubmed publisher
Maeng G, Das S, Greising S, Gong W, Singh B, Kren S, et al. Humanized skeletal muscle in MYF5/MYOD/MYF6-null pig embryos. Nat Biomed Eng. 2021;: pubmed publisher
Li N, Zhao Z, Liu P, Zheng Y, Cai S, Sun Y, et al. Upregulation of deubiquitinase USP7 by transcription factor FOXO6 promotes EC progression via targeting the JMJD3/CLU axis. Mol Ther Oncolytics. 2021;20:583-595 pubmed publisher
Duval R, Nicolas G, Willemetz A, Murakami Y, Mikdar M, Vrignaud C, et al. Inherited glycosylphosphatidylinositol defects cause the rare Emm-negative blood phenotype and developmental disorders. Blood. 2021;137:3660-3669 pubmed publisher
Burckhardt C, Minna J, Danuser G. SH3BP4 promotes neuropilin-1 and α5-integrin endocytosis and is inhibited by Akt. Dev Cell. 2021;56:1164-1181.e12 pubmed publisher
Challagulla A, Shi S, Nair K, O Neil T, Morris K, Wise T, et al. Marker counter-selection via CRISPR/Cas9 co-targeting for efficient generation of genome edited avian cell lines and germ cells. Anim Biotechnol. 2021;:1-11 pubmed publisher
Fontaine V, Duboscq Bidot L, Jouve C, Hamlin M, Curjol A, Briand V, et al. Generation of iPSC line from MYH7 R403L mutation carrier with severe hypertrophic cardiomyopathy and isogenic CRISPR/Cas9 corrected control. Stem Cell Res. 2021;52:102245 pubmed publisher
Chiola S, Napan K, Wang Y, Lazarenko R, Armstrong C, Cui J, et al. Defective AMPA-mediated synaptic transmission and morphology in human neurons with hemizygous SHANK3 deletion engrafted in mouse prefrontal cortex. Mol Psychiatry. 2021;: pubmed publisher
Soliman R, Cordero Maldonado M, Martins T, Moein M, Conrotte J, Warmack R, et al. l-Isoaspartyl Methyltransferase Deficiency in Zebrafish Leads to Impaired Calcium Signaling in the Brain. Front Genet. 2020;11:612343 pubmed publisher
Recasens Alvarez C, Alexandre C, Kirkpatrick J, Nojima H, Huels D, Snijders A, et al. Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition. Nat Cell Biol. 2021;23:127-135 pubmed publisher
Yue J, Dai Q, Hao S, Zhu S, Liu X, Tang Z, et al. Suppression of the NTS-CPS1 regulatory axis by AFF1 in lung adenocarcinoma cells. J Biol Chem. 2021;:100319 pubmed publisher
Papa G, Mallery D, Albecka A, Welch L, Cattin Ortolá J, Lupták J, et al. Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion. PLoS Pathog. 2021;17:e1009246 pubmed publisher
Feng Z, Yang Z, Gao X, Xue Y, Wang X. Resveratrol Promotes HIV-1 Tat Accumulation via AKT/FOXO1 Signaling Axis and Potentiates Vorinostat to Antagonize HIV-1 Latency. Curr HIV Res. 2021;19:238-247 pubmed publisher
Meyer N, Henkel L, Linder B, Zielke S, Tascher G, Trautmann S, et al. Autophagy activation, lipotoxicity and lysosomal membrane permeabilization synergize to promote pimozide- and loperamide-induced glioma cell death. Autophagy. 2021;:1-20 pubmed publisher
Kim J, Jung S, Kim Y, Heo H, Hong C, Seo S, et al. Identification of Cathepsin D as a Plasma Biomarker for Alzheimer's Disease. Cells. 2021;10: pubmed publisher
Lonic A, Gehling F, Belle L, Li X, Schieber N, Nguyen E, et al. Phosphorylation of PKCδ by FER tips the balance from EGFR degradation to recycling. J Cell Biol. 2021;220: pubmed publisher
Luo X, Xu Y, Li Y, Zhang G, Huang S, Liu X, et al. BAP1 deletion abrogates growth and metastasis of murine cutaneous melanoma. Melanoma Res. 2021;31:119-129 pubmed publisher
Brouwers B, Coppola I, Vints K, Dislich B, Jouvet N, Van Lommel L, et al. Loss of Furin in β-Cells Induces an mTORC1-ATF4 Anabolic Pathway That Leads to β-Cell Dysfunction. Diabetes. 2021;70:492-503 pubmed publisher
Lin Y, Haas A, Bufe A, Parbin S, Hennecke M, Voloshanenko O, et al. Wnt10b-GSK3β-dependent Wnt/STOP signaling prevents aneuploidy in human somatic cells. Life Sci Alliance. 2021;4: pubmed publisher
Bhattacharya K, Weidenauer L, Luengo T, Pieters E, Echeverria P, Bernasconi L, et al. The Hsp70-Hsp90 co-chaperone Hop/Stip1 shifts the proteostatic balance from folding towards degradation. Nat Commun. 2020;11:5975 pubmed publisher
Kang J, Kim J, Heo H, Lee J, Park K, Yoon J, et al. Identification of BAG2 and Cathepsin D as Plasma Biomarkers for Parkinson's Disease. Clin Transl Sci. 2020;: pubmed publisher
Liu W, Krishnamoorthy A, Zhao R, Cortez D. Two replication fork remodeling pathways generate nuclease substrates for distinct fork protection factors. Sci Adv. 2020;6: pubmed publisher
Takeda A, Takano N, Kokuba H, Hino H, Moriya S, Abe A, et al. Macrolide antibiotics enhance the antitumor effect of lansoprazole resulting in lysosomal membrane permeabilization‑associated cell death. Int J Oncol. 2020;57:1280-1292 pubmed publisher
Tang X, Chen Z, Tan X, Luo L, Liu X, Gong L, et al. Generation of a homozygous CRISPR/Cas9-mediated knockout H9 hESC subline for the CRB1 locus. Stem Cell Res. 2020;49:102057 pubmed publisher
Lee N, Kim S, Yu D, Woo E, Kim M, Seong G, et al. Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT). Commun Biol. 2020;3:636 pubmed publisher
Gupta M, Vethe H, Softic S, Rao T, Wagh V, Shirakawa J, et al. Leptin Receptor Signaling Regulates Protein Synthesis Pathways and Neuronal Differentiation in Pluripotent Stem Cells. Stem Cell Reports. 2020;15:1067-1079 pubmed publisher
Chang C, Xu X, Li M, Xin D, Ding L, Wang Y, et al. Pathogenic mutations reveal a role of RECQ4 in mitochondrial RNA:DNA hybrid formation and resolution. Sci Rep. 2020;10:17033 pubmed publisher
Hsiao K, Noble C, Pitman W, Yadav N, Kumar S, Keele G, et al. A Thalamic Orphan Receptor Drives Variability in Short-Term Memory. Cell. 2020;183:522-536.e19 pubmed publisher
Sen P, Ganguly P, Kulkarni K, Budhwar R, Ganguly N. Differential transcriptome analysis in HPV-positive and HPV-negative cervical cancer cells through CRISPR knockout of miR-214. J Biosci. 2020;45: pubmed
Kohama Y, Higo S, Masumura Y, Shiba M, Kondo T, Ishizu T, et al. Adeno-associated virus-mediated gene delivery promotes S-phase entry-independent precise targeted integration in cardiomyocytes. Sci Rep. 2020;10:15348 pubmed publisher
Mudla A, Jiang Y, Arimoto K, Xu B, Rajesh A, Ryan A, et al. Cell-cycle-gated feedback control mediates desensitization to interferon stimulation. elife. 2020;9: pubmed publisher
Bohlen J, Harbrecht L, Blanco S, Clemm von Hohenberg K, Fenzl K, Kramer G, et al. DENR promotes translation reinitiation via ribosome recycling to drive expression of oncogenes including ATF4. Nat Commun. 2020;11:4676 pubmed publisher
Assar E, Tumbarello D. Loss of the Essential Autophagy Regulators FIP200 or Atg5 Leads to Distinct Effects on Focal Adhesion Composition and Organization. Front Cell Dev Biol. 2020;8:733 pubmed publisher
Singh S, Thomason P, Lilla S, Schaks M, Tang Q, Goode B, et al. Cell-substrate adhesion drives Scar/WAVE activation and phosphorylation by a Ste20-family kinase, which controls pseudopod lifetime. PLoS Biol. 2020;18:e3000774 pubmed publisher
Bucko P, Garcia I, Manocha R, Bhat A, Wordeman L, Scott J. Gravin-associated kinase signaling networks coordinate γ-tubulin organization at mitotic spindle poles. J Biol Chem. 2020;295:13784-13797 pubmed publisher
Tang Q, Schaks M, Koundinya N, Yang C, Pollard L, Svitkina T, et al. WAVE1 and WAVE2 have distinct and overlapping roles in controlling actin assembly at the leading edge. Mol Biol Cell. 2020;31:2168-2178 pubmed publisher
Socha A, Yang D, Bulsiewicz A, Yaprianto K, Kupculak M, Liang C, et al. WRNIP1 Is Recruited to DNA Interstrand Crosslinks and Promotes Repair. Cell Rep. 2020;32:107850 pubmed publisher
Perez M, Ivanyuk D, Panagiotakopoulou V, Di Napoli G, Kalb S, Brunetti D, et al. Loss of function of the mitochondrial peptidase PITRM1 induces proteotoxic stress and Alzheimer's disease-like pathology in human cerebral organoids. Mol Psychiatry. 2020;: pubmed publisher
Huang J, Kan S, Sandfeld E, Dalton N, Rangel A, Chan Y, et al. CRISPR-Cas9 generated Pompe knock-in murine model exhibits early-onset hypertrophic cardiomyopathy and skeletal muscle weakness. Sci Rep. 2020;10:10321 pubmed publisher
Shvets E, Mendoza Topaz C. Tagging and Deleting of Endogenous Caveolar Components Using CRISPR/Cas9 Technology. Methods Mol Biol. 2020;2169:149-166 pubmed publisher
Liu J, Zuo Z, Sastalla I, Liu C, Jang J, Sekine Y, et al. Sequential CRISPR-Based Screens Identify LITAF and CDIP1 as the Bacillus cereus Hemolysin BL Toxin Host Receptors. Cell Host Microbe. 2020;: pubmed publisher
Meng F, Qian M, Peng B, Peng L, Wang X, Zheng K, et al. Synergy between SIRT1 and SIRT6 helps recognize DNA breaks and potentiates the DNA damage response and repair in humans and mice. elife. 2020;9: pubmed publisher
Wetzel L, Blanchard S, Rama S, Beier V, Kaufmann A, Wollert T. TECPR1 promotes aggrephagy by direct recruitment of LC3C autophagosomes to lysosomes. Nat Commun. 2020;11:2993 pubmed publisher
Cenni V, Squarzoni S, Loi M, Mattioli E, Lattanzi G, Capanni C. Emerin Phosphorylation during the Early Phase of the Oxidative Stress Response Influences Emerin-BAF Interaction and BAF Nuclear Localization. Cells. 2020;9: pubmed publisher
Seabra C, Aneichyk T, Erdin S, Tai D, de Esch C, Razaz P, et al. Transcriptional consequences of MBD5 disruption in mouse brain and CRISPR-derived neurons. Mol Autism. 2020;11:45 pubmed publisher
Ma H, Cui N, Zheng P. HOXA5 inhibits the proliferation and neoplasia of cervical cancer cells via downregulating the activity of the Wnt/β-catenin pathway and transactivating TP53. Cell Death Dis. 2020;11:420 pubmed publisher
Wu J, Xue Y, Gao X, Zhou Q. Host cell factors stimulate HIV-1 transcription by antagonizing substrate-binding function of Siah1 ubiquitin ligase to stabilize transcription elongation factor ELL2. Nucleic Acids Res. 2020;48:7321-7332 pubmed publisher
Wang H, Gong P, Li J, Fu Y, Zhou Z, Liu L. Role of CD133 in human embryonic stem cell proliferation and teratoma formation. Stem Cell Res Ther. 2020;11:208 pubmed publisher
Liang H, Zhang P, Bai H, Huang J, Yang H. TATA box-binding protein-related factor 3 drives the mesendoderm specification of human embryonic stem cells by globally interacting with the TATA box of key mesendodermal genes. Stem Cell Res Ther. 2020;11:196 pubmed publisher
Bai G, Kermi C, Stoy H, Schiltz C, Bacal J, Zaino A, et al. HLTF Promotes Fork Reversal, Limiting Replication Stress Resistance and Preventing Multiple Mechanisms of Unrestrained DNA Synthesis. Mol Cell. 2020;78:1237-1251.e7 pubmed publisher
Kelliher J, West K, Gong Q, Leung J. Histone H2A variants alpha1-extension helix directs RNF168-mediated ubiquitination. Nat Commun. 2020;11:2462 pubmed publisher
Sanna S, Esposito S, Masala A, Sini P, Nieddu G, Galioto M, et al. HDAC1 inhibition ameliorates TDP-43-induced cell death in vitro and in vivo. Cell Death Dis. 2020;11:369 pubmed publisher
Collins M, Stransky L, Forgac M. AKT Ser/Thr kinase increases V-ATPase-dependent lysosomal acidification in response to amino acid starvation in mammalian cells. J Biol Chem. 2020;295:9433-9444 pubmed publisher
Goh K, Chen J, Rocha N, Semple R. Human pluripotent stem cell-based models suggest preadipocyte senescence as a possible cause of metabolic complications of Werner and Bloom Syndromes. Sci Rep. 2020;10:7490 pubmed publisher
Hegarat N, Crncec A, Suarez Peredo Rodriguez M, Echegaray Iturra F, Gu Y, Busby O, et al. Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. EMBO J. 2020;39:e104419 pubmed publisher
Piper A, Sophocleous R, Ross S, Evesson F, Saleh O, Bournazos A, et al. Loss of calpains-1 and -2 prevents repair of plasma membrane scrape injuries, but not small pores, and induces a severe muscular dystrophy. Am J Physiol Cell Physiol. 2020;318:C1226-C1237 pubmed publisher
Kim Y, Lee J, Seppala M, Cobourne M, Kim S. Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration. Nat Commun. 2020;11:1994 pubmed publisher
Au F, Hau B, Qi R. Nek2-mediated GAS2L1 phosphorylation and centrosome-linker disassembly induce centrosome disjunction. J Cell Biol. 2020;219: pubmed publisher
Al Mahmud M, Ishii K, Bernal Lozano C, Delgado Sainz I, Toi M, Akamatsu S, et al. TDP2 suppresses genomic instability induced by androgens in the epithelial cells of prostate glands. Genes Cells. 2020;25:450-465 pubmed publisher
Faulkner J, Jiang P, Farris D, Walker R, Dai Z. CRISPR/CAS9-mediated knockout of Abi1 inhibits p185Bcr-Abl-induced leukemogenesis and signal transduction to ERK and PI3K/Akt pathways. J Hematol Oncol. 2020;13:34 pubmed publisher
Tanaka Y, Shimanaka Y, Caddeo A, Kubo T, Mao Y, Kubota T, et al. LPIAT1/MBOAT7 depletion increases triglyceride synthesis fueled by high phosphatidylinositol turnover. Gut. 2021;70:180-193 pubmed publisher
Yang Y, Li N, Qiu J, Ge H, Qin X. Identification of the Repressive Domain of the Negative Circadian Clock Component CHRONO. Int J Mol Sci. 2020;21: pubmed publisher
Meng Y, Liu Y, Dakou E, Gutierrez G, Leyns L. Polycomb group RING finger protein 5 influences several developmental signaling pathways during the in vitro differentiation of mouse embryonic stem cells. Dev Growth Differ. 2020;62:232-242 pubmed publisher
Hong A, Meng Z, Plouffe S, Lin Z, Zhang M, Guan K. Critical roles of phosphoinositides and NF2 in Hippo pathway regulation. Genes Dev. 2020;34:511-525 pubmed publisher
Takahashi H, Ranjan A, Chen S, Suzuki H, Shibata M, Hirose T, et al. The role of Mediator and Little Elongation Complex in transcription termination. Nat Commun. 2020;11:1063 pubmed publisher
Joshi A, Dai L, Liu Y, Lee J, Ghahhari N, Segala G, et al. The mitochondrial HSP90 paralog TRAP1 forms an OXPHOS-regulated tetramer and is involved in mitochondrial metabolic homeostasis. BMC Biol. 2020;18:10 pubmed publisher
Rhodes J, Feldmann A, Hernández Rodríguez B, Díaz N, Brown J, Fursova N, et al. Cohesin Disrupts Polycomb-Dependent Chromosome Interactions in Embryonic Stem Cells. Cell Rep. 2020;30:820-835.e10 pubmed publisher
Lyabin D, Eliseeva I, Smolin E, Doronin A, Budkina K, Kulakovskiy I, et al. YB-3 substitutes YB-1 in global mRNA binding. RNA Biol. 2020;17:487-499 pubmed publisher
Leidal A, Huang H, Marsh T, Solvik T, Zhang D, Ye J, et al. The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Nat Cell Biol. 2020;22:187-199 pubmed publisher
Li H, Zhan J, Zhao Y, Fan J, Yuan S, Yin Z, et al. Identification of ncRNA-Mediated Functions of Nucleus-Localized miR-320 in Cardiomyocytes. Mol Ther Nucleic Acids. 2019;19:132-143 pubmed publisher
Azouzi S, Mikdar M, Hermand P, Gautier E, Salnot V, Willemetz A, et al. Lack of the multidrug transporter MRP4/ABCC4 defines the PEL-negative blood group and impairs platelet aggregation. Blood. 2019;: pubmed publisher
Erlangga Z, Wolff K, Poth T, Peltzer A, Nahnsen S, Spielberg S, et al. Potent Antitumor Activity of Liposomal Irinotecan in an Organoid- and CRISPR-Cas9-Based Murine Model of Gallbladder Cancer. Cancers (Basel). 2019;11: pubmed publisher
Trinh D, Shirakawa R, Kimura T, Sakata N, Goto K, Horiuchi H. Inhibitor of Growth 4 (ING4) is a positive regulator of rRNA synthesis. Sci Rep. 2019;9:17235 pubmed publisher
Naito T, Ercan B, Krshnan L, Triebl A, Koh D, Wei F, et al. Movement of accessible plasma membrane cholesterol by the GRAMD1 lipid transfer protein complex. elife. 2019;8: pubmed publisher
Kowalski K, Brzoska E, Ciemerych M. The role of CXC receptors signaling in early stages of mouse embryonic stem cell differentiation. Stem Cell Res. 2019;41:101636 pubmed publisher
Weiterer S, Meier Soelch J, Georgomanolis T, Mizi A, Beyerlein A, Weiser H, et al. Distinct IL-1α-responsive enhancers promote acute and coordinated changes in chromatin topology in a hierarchical manner. EMBO J. 2019;:e101533 pubmed publisher
Marbaix A, Chehade G, Noel G, Morsomme P, Vertommen D, Bommer G, et al. Pyridoxamine-phosphate oxidases and pyridoxamine-phosphate oxidase-related proteins catalyze the oxidation of 6-NAD(P)H to NAD(P). Biochem J. 2019;476:3033-3052 pubmed publisher
Callen E, Zong D, Wu W, Wong N, Stanlie A, Ishikawa M, et al. 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination. Mol Cell. 2019;: pubmed publisher
Bersuker K, Hendricks J, Li Z, Magtanong L, Ford B, Tang P, et al. The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis. Nature. 2019;575:688-692 pubmed publisher
Chhabra S, Liu L, Goh R, Kong X, Warmflash A. Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids. PLoS Biol. 2019;17:e3000498 pubmed publisher
Laflamme C, McKeever P, Kumar R, Schwartz J, Kolahdouzan M, Chen C, et al. Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72. elife. 2019;8: pubmed publisher
Sarraf S, Sideris D, Giagtzoglou N, Ni L, Kankel M, Sen A, et al. PINK1/Parkin Influences Cell Cycle by Sequestering TBK1 at Damaged Mitochondria, Inhibiting Mitosis. Cell Rep. 2019;29:225-235.e5 pubmed publisher
Latour Y, Yoon R, Thomas S, Grant C, Li C, Sena Esteves M, et al. Human GLB1 knockout cerebral organoids: A model system for testing AAV9-mediated GLB1 gene therapy for reducing GM1 ganglioside storage in GM1 gangliosidosis. Mol Genet Metab Rep. 2019;21:100513 pubmed publisher
Ohneda K, Ohmori S, Yamamoto M. Mouse Tryptase Gene Expression is Coordinately Regulated by GATA1 and GATA2 in Bone Marrow-Derived Mast Cells. Int J Mol Sci. 2019;20: pubmed publisher
Liu N, Yin Y, Wang H, Zhou Z, Sheng X, Fu H, et al. Telomere dysfunction impairs epidermal stem cell specification and differentiation by disrupting BMP/pSmad/P63 signaling. PLoS Genet. 2019;15:e1008368 pubmed publisher
Gemble S, Simon A, Pennetier C, Dumont M, Hervé S, Meitinger F, et al. Centromere Dysfunction Compromises Mitotic Spindle Pole Integrity. Curr Biol. 2019;29:3072-3080.e5 pubmed publisher
Steinparzer I, Sedlyarov V, Rubin J, Eislmayr K, Galbraith M, Levandowski C, et al. Transcriptional Responses to IFN-γ Require Mediator Kinase-Dependent Pause Release and Mechanistically Distinct CDK8 and CDK19 Functions. Mol Cell. 2019;76:485-499.e8 pubmed publisher
Sen P, Ghosal S, Hazra R, Arega S, Mohanty R, Kulkarni K, et al. Transcriptomic analyses of gene expression by CRISPR knockout of miR-214 in cervical cancer cells. Genomics. 2019;: pubmed publisher
Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, et al. A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology. PLoS ONE. 2019;14:e0221164 pubmed publisher
Wang G, Kouwaki T, Okamoto M, Oshiumi H. Attenuation of the Innate Immune Response against Viral Infection Due to ZNF598-Promoted Binding of FAT10 to RIG-I. Cell Rep. 2019;28:1961-1970.e4 pubmed publisher
Kang J, Park J, Ko J, Kim Y. Regulation of gene expression by altered promoter methylation using a CRISPR/Cas9-mediated epigenetic editing system. Sci Rep. 2019;9:11960 pubmed publisher
Mayr Buro C, Schlereth E, Beuerlein K, Tenekeci U, Meier Soelch J, Schmitz M, et al. Single-Cell Analysis of Multiple Steps of Dynamic NF-κB Regulation in Interleukin-1α-Triggered Tumor Cells Using Proximity Ligation Assays. Cancers (Basel). 2019;11: pubmed publisher
Dewulf J, Gerin I, Rider M, Veiga da Cunha M, van Schaftingen E, Bommer G. The synthesis of branched-chain fatty acids is limited by enzymatic decarboxylation of ethyl- and methylmalonyl-CoA. Biochem J. 2019;476:2427-2447 pubmed publisher
Guo G, Wang K, Hu S, Tian T, Liu P, Mori T, et al. Autokinase Activity of Casein Kinase 1 δ/ε Governs the Period of Mammalian Circadian Rhythms. J Biol Rhythms. 2019;:748730419865406 pubmed publisher
Walia R, Ho C, Lee C, Gilch S, Schatzl H. Gene-edited murine cell lines for propagation of chronic wasting disease prions. Sci Rep. 2019;9:11151 pubmed publisher
Hollstein P, Eichner L, Brun S, Kamireddy A, Svensson R, Vera L, et al. The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive Effects of LKB1 in NSCLC. Cancer Discov. 2019;9:1606-1627 pubmed publisher
Bai H, Zhang P, Ma L, Liang H, Wei G, Yang H. SMYD2 Drives Mesendodermal Differentiation of Human Embryonic Stem Cells Through Mediating the Transcriptional Activation of Key Mesendodermal Genes. Stem Cells. 2019;: pubmed publisher
Scheimann J, Moloney R, Mahbod P, Morano R, Fitzgerald M, Hoskins O, et al. Conditional deletion of glucocorticoid receptors in rat brain results in sex-specific deficits in fear and coping behaviors. elife. 2019;8: pubmed publisher
Moskovskich A, Goldmann U, Kartnig F, Lindinger S, Konecka J, Fiume G, et al. The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection. Sci Rep. 2019;9:10471 pubmed publisher
Sangare L, Yang N, Konstantinou E, Lu D, Mukhopadhyay D, Young L, et al. Toxoplasma GRA15 Activates the NF-κB Pathway through Interactions with TNF Receptor-Associated Factors. MBio. 2019;10: pubmed publisher
Petkau N, Budak H, Zhou X, Oster H, Eichele G. Acetylation of BMAL1 by TIP60 controls BRD4-P-TEFb recruitment to circadian promoters. elife. 2019;8: pubmed publisher
Yuan F, Jin X, Li D, Song Y, Zhang N, Yang X, et al. ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint. Nucleic Acids Res. 2019;47:8096-8110 pubmed publisher
van Bemmel J, Galupa R, Gard C, Servant N, Picard C, Davies J, et al. The bipartite TAD organization of the X-inactivation center ensures opposing developmental regulation of Tsix and Xist. Nat Genet. 2019;: pubmed publisher
Alateeq S, Ovchinnikov D, Tracey T, Whitworth D, Al Rubaish A, Al Ali A, et al. Identification of on-target mutagenesis during correction of a beta-thalassemia splice mutation in iPS cells with optimised CRISPR/Cas9-double nickase reveals potential safety concerns. APL Bioeng. 2018;2:046103 pubmed publisher
Bass T, Cortez D. Quantitative phosphoproteomics reveals mitotic function of the ATR activator ETAA1. J Cell Biol. 2019;218:1235-1249 pubmed publisher
Moreno Roig E, Groot A, Yaromina A, Hendrickx T, Barbeau L, Giuranno L, et al. HIF-1α and HIF-2α Differently Regulate the Radiation Sensitivity of NSCLC Cells. Cells. 2019;8: pubmed publisher
Oakes B, Fellmann C, Rishi H, Taylor K, Ren S, Nadler D, et al. CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification. Cell. 2019;176:254-267.e16 pubmed publisher
Mohni K, Wessel S, Zhao R, Wojciechowski A, Luzwick J, Layden H, et al. HMCES Maintains Genome Integrity by Shielding Abasic Sites in Single-Strand DNA. Cell. 2019;176:144-153.e13 pubmed publisher
Menzies S, Volkmar N, van den Boomen D, Timms R, Dickson A, Nathan J, et al. The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. elife. 2018;7: pubmed publisher
Liu C, Ren L, Deng J, Wang S. LncRNA TP73-AS1 promoted the progression of lung adenocarcinoma via PI3K/AKT pathway. Biosci Rep. 2019;39: pubmed publisher
Vandemoortele G, De Sutter D, Moliere A, Pauwels J, Gevaert K, Eyckerman S. A Well-Controlled BioID Design for Endogenous Bait Proteins. J Proteome Res. 2019;18:95-106 pubmed publisher
Kéry M, Oravcova N, Radenkovic S, Iuliano F, Tomaskova J, Golias T. Pyruvate dehydrogenase kinase 1 and carbonic anhydrase IX targeting in hypoxic tumors. Neoplasma. 2018;66:63-72 pubmed publisher
Takahara M, Kunii M, Nakamura K, Harada A, Hirano T, Katoh Y, et al. C11ORF74 interacts with the IFT-A complex and participates in ciliary BBSome localization. J Biochem. 2019;165:257-267 pubmed publisher
Obiedat A, Seidel E, Mahameed M, Berhani O, Tsukerman P, Voutetakis K, et al. Transcription of the NKG2D ligand MICA is suppressed by the IRE1/XBP1 pathway of the unfolded protein response through the regulation of E2F1. FASEB J. 2019;33:3481-3495 pubmed publisher
Rata S, Suarez Peredo Rodriguez M, Joseph S, Peter N, Echegaray Iturra F, Yang F, et al. Two Interlinked Bistable Switches Govern Mitotic Control in Mammalian Cells. Curr Biol. 2018;28:3824-3832.e6 pubmed publisher
Gontan C, Mira Bontenbal H, Magaraki A, Dupont C, Barakat T, Rentmeester E, et al. REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice. Nat Commun. 2018;9:4752 pubmed publisher
Eichner L, Brun S, Herzig S, Young N, Curtis S, Shackelford D, et al. Genetic Analysis Reveals AMPK Is Required to Support Tumor Growth in Murine Kras-Dependent Lung Cancer Models. Cell Metab. 2019;29:285-302.e7 pubmed publisher
Wu A, Wu Q, Deng Y, Liu Y, Lu J, Liu L, et al. Loss of VGLL4 suppresses tumor PD-L1 expression and immune evasion. EMBO J. 2019;38: pubmed publisher
Schaks M, Singh S, Kage F, Thomason P, Klünemann T, Steffen A, et al. Distinct Interaction Sites of Rac GTPase with WAVE Regulatory Complex Have Non-redundant Functions in Vivo. Curr Biol. 2018;28:3674-3684.e6 pubmed publisher
Kloet S, Karemaker I, van Voorthuijsen L, Lindeboom R, Baltissen M, Edupuganti R, et al. NuRD-interacting protein ZFP296 regulates genome-wide NuRD localization and differentiation of mouse embryonic stem cells. Nat Commun. 2018;9:4588 pubmed publisher
CAMPBELL L, Coke L, Richie C, Fortuno L, Park A, Harvey B. Gesicle-Mediated Delivery of CRISPR/Cas9 Ribonucleoprotein Complex for Inactivating the HIV Provirus. Mol Ther. 2019;27:151-163 pubmed publisher
Motnenko A, Liang C, Yang D, Lopez Martinez D, Yoshikawa Y, Zhan B, et al. Identification of UHRF2 as a novel DNA interstrand crosslink sensor protein. PLoS Genet. 2018;14:e1007643 pubmed publisher
Li W, Yue F, Dai Y, Shi B, Xu G, Jiang X, et al. Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation. Cell Death Differ. 2018;: pubmed publisher
Iida R, Ueki M, Yasuda T. Knockout of Mpv17-Like Protein (M-LPH) Gene in Human Hepatoma Cells Results in Impairment of mtDNA Integrity through Reduction of TFAM, OGG1, and LIG3 at the Protein Levels. Oxid Med Cell Longev. 2018;2018:6956414 pubmed publisher
Wang M, Ren T, Liu H, Lim S, Lee K, Honko A, et al. Critical role for cholesterol in Lassa fever virus entry identified by a novel small molecule inhibitor targeting the viral receptor LAMP1. PLoS Pathog. 2018;14:e1007322 pubmed publisher
Guo K, Chen Q, He X, Yao K, Li Z, Liu Z, et al. Expression and significance of Cystatin-C in clear cell renal cell carcinoma. Biomed Pharmacother. 2018;107:1237-1245 pubmed publisher
Li H, Sheng C, Liu H, Wang S, Zhao J, Yang L, et al. Inhibition of HBV Expression in HBV Transgenic Mice Using AAV-Delivered CRISPR-SaCas9. Front Immunol. 2018;9:2080 pubmed publisher
Luttrell L, Wang J, Plouffe B, Smith J, Yamani L, Kaur S, et al. Manifold roles of β-arrestins in GPCR signaling elucidated with siRNA and CRISPR/Cas9. Sci Signal. 2018;11: pubmed publisher
Takao A, Yoshikawa K, Karnan S, Ota A, Uemura H, De Velasco M, et al. Generation of PTEN‑knockout (‑/‑) murine prostate cancer cells using the CRISPR/Cas9 system and comprehensive gene expression profiling. Oncol Rep. 2018;40:2455-2466 pubmed publisher
Tsujimura T, Takase O, Yoshikawa M, Sano E, Hayashi M, Takato T, et al. Control of directionality of chromatin folding for the inter- and intra-domain contacts at the Tfap2c-Bmp7 locus. Epigenetics Chromatin. 2018;11:51 pubmed publisher
Xu Y, Luo X, Fang Z, Zheng X, Zeng Y, Zhu C, et al. Transcription coactivator Cited1 acts as an inducer of trophoblast-like state from mouse embryonic stem cells through the activation of BMP signaling. Cell Death Dis. 2018;9:924 pubmed publisher
Park H, Triboulet R, Bentler M, Guda S, Du P, Xu H, et al. DROSHA Knockout Leads to Enhancement of Viral Titers for Vectors Encoding miRNA-Adapted shRNAs. Mol Ther Nucleic Acids. 2018;12:591-599 pubmed publisher
Chen S, Jang G, Hüttenhain R, Gordon D, Du D, Newton B, et al. CRL4AMBRA1 targets Elongin C for ubiquitination and degradation to modulate CRL5 signaling. EMBO J. 2018;37: pubmed publisher
Gill M, Christova T, Zhang Y, Gregorieff A, Zhang L, Narimatsu M, et al. A feed forward loop enforces YAP/TAZ signaling during tumorigenesis. Nat Commun. 2018;9:3510 pubmed publisher
Dolati S, Kage F, Mueller J, Müsken M, Kirchner M, Dittmar G, et al. On the relation between filament density, force generation, and protrusion rate in mesenchymal cell motility. Mol Biol Cell. 2018;29:2674-2686 pubmed publisher
Barnes J, Salas F, Mokhtari R, Dolstra H, Pedrosa E, Lachman H. Modeling the neuropsychiatric manifestations of Lowe syndrome using induced pluripotent stem cells: defective F-actin polymerization and WAVE-1 expression in neuronal cells. Mol Autism. 2018;9:44 pubmed publisher
Leung J, Emery L, Miller K. CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A. Methods Mol Biol. 2018;1832:255-269 pubmed publisher
Shi L, Tang X, Qian M, Liu Z, Meng F, Fu L, et al. A SIRT1-centered circuitry regulates breast cancer stemness and metastasis. Oncogene. 2018;37:6299-6315 pubmed publisher
Du J, Kirk B, Zeng J, Ma J, Wang Q. Three classes of response elements for human PRC2 and MLL1/2-Trithorax complexes. Nucleic Acids Res. 2018;46:8848-8864 pubmed publisher
Seeley J, Baker R, Mohamed G, Bruns T, Hayden M, Deshmukh S, et al. Induction of innate immune memory via microRNA targeting of chromatin remodelling factors. Nature. 2018;559:114-119 pubmed publisher
Yu D, Liu R, Yang G, Zhou Q. The PARP1-Siah1 Axis Controls HIV-1 Transcription and Expression of Siah1 Substrates. Cell Rep. 2018;23:3741-3749 pubmed publisher
Stewart S, Ashkenazi A, Williamson A, Rubinsztein D, Moreau K. Transbilayer phospholipid movement facilitates the translocation of annexin across membranes. J Cell Sci. 2018;131: pubmed publisher
Zhang J, Lan Y, Li M, Lamers M, Fusade Boyer M, Klemm E, et al. Flaviviruses Exploit the Lipid Droplet Protein AUP1 to Trigger Lipophagy and Drive Virus Production. Cell Host Microbe. 2018;23:819-831.e5 pubmed publisher
Künzel U, Grieve A, Meng Y, Sieber B, Cowley S, Freeman M. FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex. elife. 2018;7: pubmed publisher
Papasergi Scott M, Stoveken H, MacConnachie L, Chan P, Gabay M, Wong D, et al. Dual phosphorylation of Ric-8A enhances its ability to mediate G protein α subunit folding and to stimulate guanine nucleotide exchange. Sci Signal. 2018;11: pubmed publisher
Dimitrova E, Kondo T, Feldmann A, Nakayama M, Koseki Y, Konietzny R, et al. FBXL19 recruits CDK-Mediator to CpG islands of developmental genes priming them for activation during lineage commitment. elife. 2018;7: pubmed publisher
Plouffe S, Lin K, Moore J, Tan F, Ma S, Ye Z, et al. The Hippo pathway effector proteins YAP and TAZ have both distinct and overlapping functions in the cell. J Biol Chem. 2018;293:11230-11240 pubmed publisher
Kaneshiro N, Imaoka R, Komai M, Kashiyama T, Sakurai T, Uehara T, et al. Functional analysis of juxta- and intra-membrane domains of murine APP by genome editing in Neuro2a cells. Biochem Biophys Res Commun. 2018;: pubmed publisher
Sorbara M, Foerster E, Tsalikis J, Abdel Nour M, Mangiapane J, Sirluck Schroeder I, et al. Complement C3 Drives Autophagy-Dependent Restriction of Cyto-invasive Bacteria. Cell Host Microbe. 2018;23:644-652.e5 pubmed publisher
Zhuang L, Jang Y, Park Y, Lee J, Jain S, Froimchuk E, et al. Depletion of Nsd2-mediated histone H3K36 methylation impairs adipose tissue development and function. Nat Commun. 2018;9:1796 pubmed publisher
Qian M, Liu Z, Peng L, Tang X, Meng F, Ao Y, et al. Boosting ATM activity alleviates aging and extends lifespan in a mouse model of progeria. elife. 2018;7: pubmed publisher
Aboudehen K, Farahani S, Kanchwala M, Chan S, Avdulov S, Mickelson A, et al. Long noncoding RNA Hoxb3os is dysregulated in autosomal dominant polycystic kidney disease and regulates mTOR signaling. J Biol Chem. 2018;293:9388-9398 pubmed publisher
Fang F, Angulo B, Xia N, Sukhwani M, Wang Z, Carey C, et al. A PAX5-OCT4-PRDM1 developmental switch specifies human primordial germ cells. Nat Cell Biol. 2018;20:655-665 pubmed publisher
Wu Z, Zhao J, Qiu M, Mi Z, Meng M, Guo Y, et al. CRISPR/Cas9 Mediated GFP Knock-in at the MAP1LC3B Locus in 293FT Cells Is Better for Bona Fide Monitoring Cellular Autophagy. Biotechnol J. 2018;13:e1700674 pubmed publisher
Kim H, Tan Y, Ma W, Merkurjev D, Destici E, Ma Q, et al. Pluripotency factors functionally premark cell-type-restricted enhancers in ES cells. Nature. 2018;556:510-514 pubmed publisher
Lu J, Liu L, Zheng M, Li X, Wu A, Wu Q, et al. MEKK2 and MEKK3 suppress Hedgehog pathway-dependent medulloblastoma by inhibiting GLI1 function. Oncogene. 2018;37:3864-3878 pubmed publisher
Finley L, Vardhana S, Carey B, Alonso Curbelo D, Koche R, Chen Y, et al. Pluripotency transcription factors and Tet1/2 maintain Brd4-independent stem cell identity. Nat Cell Biol. 2018;20:565-574 pubmed publisher
Hong S, Song W, Zushin P, Liu B, Jedrychowski M, Mina A, et al. Phosphorylation of Beta-3 adrenergic receptor at serine 247 by ERK MAP kinase drives lipolysis in obese adipocytes. Mol Metab. 2018;12:25-38 pubmed publisher
Haahr P, Borgermann N, Guo X, Typas D, Achuthankutty D, Hoffmann S, et al. ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability. Mol Cell. 2018;70:165-174.e6 pubmed publisher
Weng L, Han Y, Enomoto A, Kitaura Y, Nagamori S, Kanai Y, et al. Negative regulation of amino acid signaling by MAPK-regulated 4F2hc/Girdin complex. PLoS Biol. 2018;16:e2005090 pubmed publisher
Huang E, To M, Tran E, Dionisio L, Cho H, Baney K, et al. A VCP inhibitor substrate trapping approach (VISTA) enables proteomic profiling of endogenous ERAD substrates. Mol Biol Cell. 2018;29:1021-1030 pubmed publisher
Burgess S, Mukherjee M, Sabir S, Joseph N, Gutiérrez Caballero C, Richards M, et al. Mitotic spindle association of TACC3 requires Aurora-A-dependent stabilization of a cryptic α-helix. EMBO J. 2018;37: pubmed publisher
Palstra R, De Crignis E, Röling M, van Staveren T, Kan T, van Ijcken W, et al. Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1. Sci Adv. 2018;4:e1701729 pubmed publisher
Zhenilo S, Deyev I, Litvinova E, Zhigalova N, Kaplun D, Sokolov A, et al. DeSUMOylation switches Kaiso from activator to repressor upon hyperosmotic stress. Cell Death Differ. 2018;25:1938-1951 pubmed publisher
Karemaker I, Vermeulen M. ZBTB2 reads unmethylated CpG island promoters and regulates embryonic stem cell differentiation. EMBO Rep. 2018;19: pubmed publisher
Chen J, Lin W, Chen Z, Liu H. PARP-1-dependent recruitment of cold-inducible RNA-binding protein promotes double-strand break repair and genome stability. Proc Natl Acad Sci U S A. 2018;115:E1759-E1768 pubmed publisher
Stielow B, Finkernagel F, Stiewe T, Nist A, Suske G. MGA, L3MBTL2 and E2F6 determine genomic binding of the non-canonical Polycomb repressive complex PRC1.6. PLoS Genet. 2018;14:e1007193 pubmed publisher
Glaeser K, Urban M, Fenech E, Voloshanenko O, Kranz D, Lari F, et al. ERAD-dependent control of the Wnt secretory factor Evi. EMBO J. 2018;37: pubmed publisher
Allende M, Cook E, Larman B, Nugent A, Brady J, Golebiowski D, et al. Cerebral organoids derived from Sandhoff disease-induced pluripotent stem cells exhibit impaired neurodifferentiation. J Lipid Res. 2018;59:550-563 pubmed publisher
Lopes F, Keita A, Saxena A, Reyes J, Mancini N, Al Rajabi A, et al. ER-stress mobilization of death-associated protein kinase-1-dependent xenophagy counteracts mitochondria stress-induced epithelial barrier dysfunction. J Biol Chem. 2018;293:3073-3087 pubmed publisher
Pusapati G, Kong J, Patel B, Krishnan A, Sagner A, Kinnebrew M, et al. CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog. Dev Cell. 2018;44:113-129.e8 pubmed publisher
Bersuker K, Peterson C, To M, Sahl S, Savikhin V, Grossman E, et al. A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes. Dev Cell. 2018;44:97-112.e7 pubmed publisher
Zuin J, Casa V, Pozojevic J, Kolovos P, van den Hout M, van Ijcken W, et al. Regulation of the cohesin-loading factor NIPBL: Role of the lncRNA NIPBL-AS1 and identification of a distal enhancer element. PLoS Genet. 2017;13:e1007137 pubmed publisher
Alvarez Fernandez M, Sanz Flores M, Sanz Castillo B, Salazar Roa M, Partida D, Zapatero Solana E, et al. Therapeutic relevance of the PP2A-B55 inhibitory kinase MASTL/Greatwall in breast cancer. Cell Death Differ. 2018;25:828-840 pubmed publisher
Takahara M, Katoh Y, Nakamura K, Hirano T, Sugawa M, Tsurumi Y, et al. Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis. Hum Mol Genet. 2018;27:516-528 pubmed publisher
Batenburg N, Walker J, Noordermeer S, Moatti N, Durocher D, Zhu X. ATM and CDK2 control chromatin remodeler CSB to inhibit RIF1 in DSB repair pathway choice. Nat Commun. 2017;8:1921 pubmed publisher
Del Castillo Velasco Herrera M, van der Weyden L, Nsengimana J, Speak A, Sjöberg M, Bishop D, et al. Comparative genomics reveals that loss of lunatic fringe (LFNG) promotes melanoma metastasis. Mol Oncol. 2018;12:239-255 pubmed publisher
Wang J, Hanada K, Staus D, Makara M, Dahal G, Chen Q, et al. Gαi is required for carvedilol-induced β1 adrenergic receptor β-arrestin biased signaling. Nat Commun. 2017;8:1706 pubmed publisher
Egami Y, Kawai K, Araki N. RhoC regulates the actin remodeling required for phagosome formation during Fc?R-mediated phagocytosis. J Cell Sci. 2017;130:4168-4179 pubmed publisher
Yau E, Kummetha I, Lichinchi G, Tang R, Zhang Y, Rana T. Genome-Wide CRISPR Screen for Essential Cell Growth Mediators in Mutant KRAS Colorectal Cancers. Cancer Res. 2017;77:6330-6339 pubmed publisher
Yeow I, Howard G, Chadwick J, Mendoza Topaz C, Hansen C, Nichols B, et al. EHD Proteins Cooperate to Generate Caveolar Clusters and to Maintain Caveolae during Repeated Mechanical Stress. Curr Biol. 2017;27:2951-2962.e5 pubmed publisher
Serikawa T, Eberle J, Kurreck J. Effects of genomic disruption of a guanine quadruplex in the 5' UTR of the Bcl-2 mRNA in melanoma cells. FEBS Lett. 2017;591:3649-3659 pubmed publisher
Ren G, Jin W, Cui K, Rodrigez J, Hu G, Zhang Z, et al. CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression. Mol Cell. 2017;67:1049-1058.e6 pubmed publisher
Vujanovic M, Krietsch J, Raso M, Terraneo N, Zellweger R, Schmid J, et al. Replication Fork Slowing and Reversal upon DNA Damage Require PCNA Polyubiquitination and ZRANB3 DNA Translocase Activity. Mol Cell. 2017;67:882-890.e5 pubmed publisher
López Jiménez A, Basak T, Vanacore R. Proteolytic processing of lysyl oxidase-like-2 in the extracellular matrix is required for crosslinking of basement membrane collagen IV. J Biol Chem. 2017;292:16970-16982 pubmed publisher
Kage F, Steffen A, Ellinger A, Ranftler C, Gehre C, Brakebusch C, et al. FMNL2 and -3 regulate Golgi architecture and anterograde transport downstream of Cdc42. Sci Rep. 2017;7:9791 pubmed publisher
Johansen A, Molenaar B, Versteeg D, Leitoguinho A, Demkes C, Spanjaard B, et al. Postnatal Cardiac Gene Editing Using CRISPR/Cas9 With AAV9-Mediated Delivery of Short Guide RNAs Results in Mosaic Gene Disruption. Circ Res. 2017;121:1168-1181 pubmed publisher
Ishizu T, Higo S, Masumura Y, Kohama Y, Shiba M, Higo T, et al. Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes. Sci Rep. 2017;7:9363 pubmed publisher
Achleitner M, Kleefisch M, Hennig A, Peschke K, Polikarpova A, Oertel R, et al. Lack of Trex1 Causes Systemic Autoimmunity despite the Presence of Antiretroviral Drugs. J Immunol. 2017;199:2261-2269 pubmed publisher
Tang X, Shi L, Xie N, Liu Z, Qian M, Meng F, et al. SIRT7 antagonizes TGF-? signaling and inhibits breast cancer metastasis. Nat Commun. 2017;8:318 pubmed publisher
Westrick R, Tomberg K, Siebert A, Zhu G, Winn M, Dobies S, et al. Sensitized mutagenesis screen in Factor V Leiden mice identifies thrombosis suppressor loci. Proc Natl Acad Sci U S A. 2017;114:9659-9664 pubmed publisher
Forrest M, Zhang H, Moy W, McGowan H, Leites C, Dionisio L, et al. Open Chromatin Profiling in hiPSC-Derived Neurons Prioritizes Functional Noncoding Psychiatric Risk Variants and Highlights Neurodevelopmental Loci. Cell Stem Cell. 2017;21:305-318.e8 pubmed publisher
Postler T, Pantry S, Desrosiers R, Ghosh S. Identification and characterization of a long non-coding RNA up-regulated during HIV-1 infection. Virology. 2017;511:30-39 pubmed publisher
Fu Y, Zhou Z, Wang H, Gong P, Guo R, Wang J, et al. IFITM1 suppresses expression of human endogenous retroviruses in human embryonic stem cells. FEBS Open Bio. 2017;7:1102-1110 pubmed publisher
Scott C, Marsden A, Rebagliati M, Zhang Q, Chamling X, Searby C, et al. Nuclear/cytoplasmic transport defects in BBS6 underlie congenital heart disease through perturbation of a chromatin remodeling protein. PLoS Genet. 2017;13:e1006936 pubmed publisher
Lin K, Moroishi T, Meng Z, Jeong H, Plouffe S, Sekido Y, et al. Regulation of Hippo pathway transcription factor TEAD by p38 MAPK-induced cytoplasmic translocation. Nat Cell Biol. 2017;19:996-1002 pubmed publisher
Banks C, Rodriguez N, Gashler K, Pandya R, Mortenson J, Whited M, et al. Acylation of Superoxide Dismutase 1 (SOD1) at K122 Governs SOD1-Mediated Inhibition of Mitochondrial Respiration. Mol Cell Biol. 2017;37: pubmed publisher
Zheng F, Zhang Z, Flamini V, Jiang W, Cui Y. The Axis of CXCR4/SDF-1 Plays a Role in Colon Cancer Cell Adhesion Through Regulation of the AKT and IGF1R Signalling Pathways. Anticancer Res. 2017;37:4361-4369 pubmed
Voloshanenko O, Gmach P, Winter J, Kranz D, Boutros M. Mapping of Wnt-Frizzled interactions by multiplex CRISPR targeting of receptor gene families. FASEB J. 2017;31:4832-4844 pubmed publisher
Kandasamy R, Vladimer G, Snijder B, Müller A, Rebsamen M, Bigenzahn J, et al. A time-resolved molecular map of the macrophage response to VSV infection. NPJ Syst Biol Appl. 2016;2:16027 pubmed publisher
Zhang C, Hawley S, Zong Y, Li M, Wang Z, Gray A, et al. Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK. Nature. 2017;548:112-116 pubmed publisher
Nabet B, Qiu Y, Shabason J, Wu T, Yoon T, Kim B, et al. Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer. Cell. 2017;170:352-366.e13 pubmed publisher
Gueroussov S, Weatheritt R, O Hanlon D, Lin Z, Narula A, Gingras A, et al. Regulatory Expansion in Mammals of Multivalent hnRNP Assemblies that Globally Control Alternative Splicing. Cell. 2017;170:324-339.e23 pubmed publisher
Park J, Son Y, Yoo B, Yang W, Kim J, Kim J, et al. Syk Plays a Critical Role in the Expression and Activation of IRAK1 in LPS-Treated Macrophages. Mediators Inflamm. 2017;2017:1506248 pubmed publisher
Biering S, Choi J, Halstrom R, Brown H, Beatty W, Lee S, et al. Viral Replication Complexes Are Targeted by LC3-Guided Interferon-Inducible GTPases. Cell Host Microbe. 2017;22:74-85.e7 pubmed publisher
White C, Vanyai H, See H, Johnstone E, Pfleger K. Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time. Sci Rep. 2017;7:3187 pubmed publisher
Tchasovnikarova I, Timms R, Douse C, Roberts R, Dougan G, Kingston R, et al. Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2. Nat Genet. 2017;49:1035-1044 pubmed publisher
Jensen K, Fløe L, Petersen T, Huang J, Xu F, Bolund L, et al. Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency. FEBS Lett. 2017;591:1892-1901 pubmed publisher
Sun L, Yang X, Li Q, Zeng P, Liu Y, Liu L, et al. Activation of Adiponectin Receptor Regulates Proprotein Convertase Subtilisin/Kexin Type 9 Expression and Inhibits Lesions in ApoE-Deficient Mice. Arterioscler Thromb Vasc Biol. 2017;37:1290-1300 pubmed publisher
Chuh K, Batt A, Zaro B, Darabedian N, Marotta N, Brennan C, et al. The New Chemical Reporter 6-Alkynyl-6-deoxy-GlcNAc Reveals O-GlcNAc Modification of the Apoptotic Caspases That Can Block the Cleavage/Activation of Caspase-8. J Am Chem Soc. 2017;139:7872-7885 pubmed publisher
McGee L, Jiang A, Lan Y. Golga5 is dispensable for mouse embryonic development and postnatal survival. Genesis. 2017;55: pubmed publisher
Yang F, Ma Q, Liu Z, Li W, Tan Y, Jin C, et al. Glucocorticoid Receptor:MegaTrans Switching Mediates the Repression of an ERα-Regulated Transcriptional Program. Mol Cell. 2017;66:321-331.e6 pubmed publisher
Gouti M, Delile J, Stamataki D, Wymeersch F, Huang Y, Kleinjung J, et al. A Gene Regulatory Network Balances Neural and Mesoderm Specification during Vertebrate Trunk Development. Dev Cell. 2017;41:243-261.e7 pubmed publisher
Nishijima Y, Hagiya Y, Kubo T, Takei R, Katoh Y, Nakayama K. RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly. Mol Biol Cell. 2017;28:1652-1666 pubmed publisher
Yu R, Liu T, Jin S, Ning C, Lendahl U, Nister M, et al. MIEF1/2 function as adaptors to recruit Drp1 to mitochondria and regulate the association of Drp1 with Mff. Sci Rep. 2017;7:880 pubmed publisher
Li H, Sheng C, Wang S, Yang L, Liang Y, Huang Y, et al. Removal of Integrated Hepatitis B Virus DNA Using CRISPR-Cas9. Front Cell Infect Microbiol. 2017;7:91 pubmed publisher
Bhujabal Z, Birgisdottir A, Sjøttem E, Brenne H, Øvervatn A, Habisov S, et al. FKBP8 recruits LC3A to mediate Parkin-independent mitophagy. EMBO Rep. 2017;18:947-961 pubmed publisher
Basu S, ADAMS L, Guhathakurta S, Kim Y. A novel tool for monitoring endogenous alpha-synuclein transcription by NanoLuciferase tag insertion at the 3'end using CRISPR-Cas9 genome editing technique. Sci Rep. 2017;8:45883 pubmed publisher
Donnio L, Bidon B, Hashimoto S, May M, Epanchintsev A, Ryan C, et al. MED12-related XLID disorders are dose-dependent of immediate early genes (IEGs) expression. Hum Mol Genet. 2017;26:2062-2075 pubmed publisher
de Jaime Soguero A, Aulicino F, Ertaylan G, Griego A, Cerrato A, Tallam A, et al. Wnt/Tcf1 pathway restricts embryonic stem cell cycle through activation of the Ink4/Arf locus. PLoS Genet. 2017;13:e1006682 pubmed publisher
Bruun K, Beach J, Heissler S, Remmert K, Sellers J, Hammer J. Re-evaluating the roles of myosin 18A? and F-actin in determining Golgi morphology. Cytoskeleton (Hoboken). 2017;74:205-218 pubmed publisher
Cui J, Chew S, Shi Y, Gong Z, Shen H. CRISPR system for genome engineering: the application for autophagy study. BMB Rep. 2017;50:247-256 pubmed
Lin L, Petersen T, Jensen K, Bolund L, Kühn R, Luo Y. Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells. J Biotechnol. 2017;247:42-49 pubmed publisher
Ito K, Eguchi Y, Imagawa Y, Akai S, Mochizuki H, Tsujimoto Y. MPP+ induces necrostatin-1- and ferrostatin-1-sensitive necrotic death of neuronal SH-SY5Y cells. Cell Death Discov. 2017;3:17013 pubmed publisher
Leung J, Makharashvili N, Agarwal P, Chiu L, Pourpre R, Cammarata M, et al. ZMYM3 regulates BRCA1 localization at damaged chromatin to promote DNA repair. Genes Dev. 2017;31:260-274 pubmed publisher
Heidary Arash E, Shiban A, Song S, Attisano L. MARK4 inhibits Hippo signaling to promote proliferation and migration of breast cancer cells. EMBO Rep. 2017;18:420-436 pubmed publisher
Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, et al. Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated, homology-independent knock-in system. Mol Biol Cell. 2017;28:898-906 pubmed publisher
Masters T, Buss F. Filopodia formation and endosome clustering induced by mutant plus-end-directed myosin VI. Proc Natl Acad Sci U S A. 2017;114:1595-1600 pubmed publisher
Qi S, Li Z, Schulze Gahmen U, Stjepanovic G, Zhou Q, Hurley J. Structural basis for ELL2 and AFF4 activation of HIV-1 proviral transcription. Nat Commun. 2017;8:14076 pubmed publisher
Funabashi T, Katoh Y, Michisaka S, Terada M, Sugawa M, Nakayama K. Ciliary entry of KIF17 is dependent on its binding to the IFT-B complex via IFT46-IFT56 as well as on its nuclear localization signal. Mol Biol Cell. 2017;28:624-633 pubmed publisher
Nejedlá M, Li Z, Masser A, Biancospino M, Spiess M, Mackowiak S, et al. A Fluorophore Fusion Construct of Human Profilin I with Non-Compromised Poly(L-Proline) Binding Capacity Suitable for Imaging. J Mol Biol. 2017;429:964-976 pubmed publisher
Li M, Lau Z, Cheung P, Aguilar E, Schneider W, Bozzacco L, et al. TRIM25 Enhances the Antiviral Action of Zinc-Finger Antiviral Protein (ZAP). PLoS Pathog. 2017;13:e1006145 pubmed publisher
Ouellet D, Cherif K, Rousseau J, Tremblay J. Deletion of the GAA repeats from the human frataxin gene using the CRISPR-Cas9 system in YG8R-derived cells and mouse models of Friedreich ataxia. Gene Ther. 2017;24:265-274 pubmed publisher
Makino S, Fukumura R, Gondo Y. Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9. Sci Rep. 2016;6:39608 pubmed publisher
Kapahnke M, Banning A, Tikkanen R. Random Splicing of Several Exons Caused by a Single Base Change in the Target Exon of CRISPR/Cas9 Mediated Gene Knockout. Cells. 2016;5: pubmed
Ota A, Nakao H, Sawada Y, Karnan S, Wahiduzzaman M, Inoue T, et al. ?40p53? suppresses tumor cell proliferation and induces cellular senescence in hepatocellular carcinoma cells. J Cell Sci. 2017;130:614-625 pubmed publisher
Phelps M, Bailey J, Vleeshouwer Neumann T, Chen E. CRISPR screen identifies the NCOR/HDAC3 complex as a major suppressor of differentiation in rhabdomyosarcoma. Proc Natl Acad Sci U S A. 2016;113:15090-15095 pubmed publisher
Hirano T, Katoh Y, Nakayama K. Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. Mol Biol Cell. 2017;28:429-439 pubmed publisher
Nozaki S, Katoh Y, Terada M, Michisaka S, Funabashi T, Takahashi S, et al. Regulation of ciliary retrograde protein trafficking by the Joubert syndrome proteins ARL13B and INPP5E. J Cell Sci. 2017;130:563-576 pubmed publisher
Plouffe S, Meng Z, Lin K, Lin B, Hong A, Chun J, et al. Characterization of Hippo Pathway Components by Gene Inactivation. Mol Cell. 2016;64:993-1008 pubmed publisher
Moroishi T, Hayashi T, Pan W, Fujita Y, Holt M, Qin J, et al. The Hippo Pathway Kinases LATS1/2 Suppress Cancer Immunity. Cell. 2016;167:1525-1539.e17 pubmed publisher
Ishida N, Nakagawa T, Iemura S, Yasui A, Shima H, Katoh Y, et al. Ubiquitylation of Ku80 by RNF126 Promotes Completion of Nonhomologous End Joining-Mediated DNA Repair. Mol Cell Biol. 2017;37: pubmed publisher
Herring A, Messana A, Bara A, Hazelbaker D, Eggan K, Barrett L. Generation of a TLE1 homozygous knockout human embryonic stem cell line using CRISPR-Cas9. Stem Cell Res. 2016;17:430-432 pubmed publisher
Pires C, Schmid B, Petræus C, Poon A, Nimsanor N, Nielsen T, et al. Generation of a gene-corrected isogenic control cell line from an Alzheimer's disease patient iPSC line carrying a A79V mutation in PSEN1. Stem Cell Res. 2016;17:285-288 pubmed publisher
Köferle A, Worf K, Breunig C, Baumann V, Herrero J, Wiesbeck M, et al. CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening. BMC Genomics. 2016;17:917 pubmed
Maillard P, van der Veen A, Deddouche Grass S, Rogers N, Merits A, Reis E Sousa C. Inactivation of the type I interferon pathway reveals long double-stranded RNA-mediated RNA interference in mammalian cells. EMBO J. 2016;35:2505-2518 pubmed
Mardakheh F, Self A, Marshall C. RHO binding to FAM65A regulates Golgi reorientation during cell migration. J Cell Sci. 2016;129:4466-4479 pubmed
Martins R, Maier J, Gorki A, Huber K, Sharif O, Starkl P, et al. Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions. Nat Immunol. 2016;17:1361-1372 pubmed publisher
Sato K, Kimura M, Sugiyama K, Nishikawa M, Okano Y, Nagaoka H, et al. Four-and-a-half LIM Domains 1 (FHL1) Protein Interacts with the Rho Guanine Nucleotide Exchange Factor PLEKHG2/FLJ00018 and Regulates Cell Morphogenesis. J Biol Chem. 2016;291:25227-25238 pubmed
Tanaka Y, Ono N, Shima T, Tanaka G, Katoh Y, Nakayama K, et al. The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane. Mol Biol Cell. 2016;27:3883-3893 pubmed
Bass T, Luzwick J, Kavanaugh G, Carroll C, Dungrawala H, Glick G, et al. ETAA1 acts at stalled replication forks to maintain genome integrity. Nat Cell Biol. 2016;18:1185-1195 pubmed publisher
Rose N, King H, Blackledge N, Fursova N, Ember K, Fischer R, et al. RYBP stimulates PRC1 to shape chromatin-based communication between Polycomb repressive complexes. elife. 2016;5: pubmed publisher
Ha N, Long J, Cai Q, Shu X, Hunter K. The Circadian Rhythm Gene Arntl2 Is a Metastasis Susceptibility Gene for Estrogen Receptor-Negative Breast Cancer. PLoS Genet. 2016;12:e1006267 pubmed publisher
Rösch K, Kwiatkowski M, Hofmann S, Schöbel A, Grüttner C, Wurlitzer M, et al. Quantitative Lipid Droplet Proteome Analysis Identifies Annexin A3 as a Cofactor for HCV Particle Production. Cell Rep. 2016;16:3219-3231 pubmed publisher
Sessions J, Skousen C, Price K, Hanks B, Hope S, Alder J, et al. CRISPR-Cas9 directed knock-out of a constitutively expressed gene using lance array nanoinjection. Springerplus. 2016;5:1521 pubmed publisher
Makino A, Hullin Matsuda F, Murate M, Abe M, Tomishige N, Fukuda M, et al. Acute accumulation of free cholesterol induces the degradation of perilipin 2 and Rab18-dependent fusion of ER and lipid droplets in cultured human hepatocytes. Mol Biol Cell. 2016;27:3293-3304 pubmed
Li H, Sheng C, Liu H, Liu G, Du X, Du J, et al. An Effective Molecular Target Site in Hepatitis B Virus S Gene for Cas9 Cleavage and Mutational Inactivation. Int J Biol Sci. 2016;12:1104-13 pubmed publisher
Zhang X, Chen M, Wu X, Kodani A, Fan J, Doan R, et al. Cell-Type-Specific Alternative Splicing Governs Cell Fate in the Developing Cerebral Cortex. Cell. 2016;166:1147-1162.e15 pubmed publisher
Murray D, Jahnel M, Lauer J, Avellaneda M, Brouilly N, Cezanne A, et al. An endosomal tether undergoes an entropic collapse to bring vesicles together. Nature. 2016;537:107-111 pubmed publisher
Henning A, Haag J, Smits B, Gould M. The Non-coding Mammary Carcinoma Susceptibility Locus, Mcs5c, Regulates Pappa Expression via Age-Specific Chromatin Folding and Allele-Dependent DNA Methylation. PLoS Genet. 2016;12:e1006261 pubmed publisher
Oji A, Noda T, Fujihara Y, Miyata H, Kim Y, Muto M, et al. CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice. Sci Rep. 2016;6:31666 pubmed publisher
Walton J, Blagih J, Ennis D, Leung E, Dowson S, Farquharson M, et al. CRISPR/Cas9-Mediated Trp53 and Brca2 Knockout to Generate Improved Murine Models of Ovarian High-Grade Serous Carcinoma. Cancer Res. 2016;76:6118-6129 pubmed
Stanley S, Gable D, Wagner C, Carlile T, Hanumanthu V, Podlevsky J, et al. Loss-of-function mutations in the RNA biogenesis factor NAF1 predispose to pulmonary fibrosis-emphysema. Sci Transl Med. 2016;8:351ra107 pubmed publisher
Kubota Y, Nomura K, Katoh Y, Yamashita R, Kaneko K, Furuyama K. Novel Mechanisms for Heme-dependent Degradation of ALAS1 Protein as a Component of Negative Feedback Regulation of Heme Biosynthesis. J Biol Chem. 2016;291:20516-29 pubmed publisher
Robijns J, Molenberghs F, Sieprath T, Corne T, Verschuuren M, De Vos W. In silico synchronization reveals regulators of nuclear ruptures in lamin A/C deficient model cells. Sci Rep. 2016;6:30325 pubmed publisher
Kawaguchi K, Okamoto T, Morita M, Imanaka T. Translocation of the ABC transporter ABCD4 from the endoplasmic reticulum to lysosomes requires the escort protein LMBD1. Sci Rep. 2016;6:30183 pubmed publisher
Meitinger F, Anzola J, Kaulich M, Richardson A, Stender J, Benner C, et al. 53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. J Cell Biol. 2016;214:155-66 pubmed publisher
Lambrus B, Daggubati V, Uetake Y, Scott P, Clutario K, Sluder G, et al. A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis. J Cell Biol. 2016;214:143-53 pubmed publisher
Liang C, Li Z, Lopez Martinez D, Nicholson W, Venien Bryan C, Cohn M. The FANCD2-FANCI complex is recruited to DNA interstrand crosslinks before monoubiquitination of FANCD2. Nat Commun. 2016;7:12124 pubmed publisher
Baumann S, Hennet T. Collagen Accumulation in Osteosarcoma Cells lacking GLT25D1 Collagen Galactosyltransferase. J Biol Chem. 2016;291:18514-24 pubmed publisher
Shao W, Machamer C, Espenshade P. Fatostatin blocks ER exit of SCAP but inhibits cell growth in a SCAP-independent manner. J Lipid Res. 2016;57:1564-73 pubmed publisher
Liu X, Chen L, Ge J, Yan C, Huang Z, Hu J, et al. The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner. Cancer Res. 2016;76:4897-907 pubmed publisher
Hall M, Weidner D, Zhu Y, Dayal S, Whitman A, Schwalbe R. Predominant Expression of Hybrid N-Glycans Has Distinct Cellular Roles Relative to Complex and Oligomannose N-Glycans. Int J Mol Sci. 2016;17: pubmed publisher
Collard F, Baldin F, Gerin I, Bolsée J, Noël G, Graff J, et al. A conserved phosphatase destroys toxic glycolytic side products in mammals and yeast. Nat Chem Biol. 2016;12:601-7 pubmed publisher
Li T, Qi S, Unger M, Hou Y, Deng Q, Liu J, et al. Immuno-targeting the multifunctional CD38 using nanobody. Sci Rep. 2016;6:27055 pubmed publisher
Lan Y, Zhang N, Liu H, Xu J, Jiang R. Golgb1 regulates protein glycosylation and is crucial for mammalian palate development. Development. 2016;143:2344-55 pubmed publisher
Han T, Goralski M, Capota E, Padrick S, Kim J, Xie Y, et al. The antitumor toxin CD437 is a direct inhibitor of DNA polymerase ?. Nat Chem Biol. 2016;12:511-5 pubmed publisher
An L, Hu Y, Chang S, Zhu X, Ling P, Zhang F, et al. Efficient generation of FVII gene knockout mice using CRISPR/Cas9 nuclease and truncated guided RNAs. Sci Rep. 2016;6:25199 pubmed publisher
Crevenna A, Blank B, Maiser A, Emin D, Prescher J, Beck G, et al. Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network. J Cell Biol. 2016;213:305-14 pubmed publisher
Ohno S, Itano K, Harada Y, Asada K, Oikawa K, Kashiwazako M, et al. Development of Novel Small Hairpin RNAs That do not Require Processing by Dicer or AGO2. Mol Ther. 2016;24:1278-89 pubmed publisher
Ha J, Byun J, Ahn D. Overcoming doxorubicin resistance of cancer cells by Cas9-mediated gene disruption. Sci Rep. 2016;6:22847 pubmed publisher
Yang D, Scavuzzo M, Chmielowiec J, Sharp R, Bajić A, Borowiak M. Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases. Sci Rep. 2016;6:21264 pubmed publisher
Stewart Ornstein J, Lahav G. Dynamics of CDKN1A in Single Cells Defined by an Endogenous Fluorescent Tagging Toolkit. Cell Rep. 2016;14:1800-1811 pubmed publisher
Tai D, Ragavendran A, Manavalan P, Stortchevoi A, Seabra C, Erdin S, et al. Engineering microdeletions and microduplications by targeting segmental duplications with CRISPR. Nat Neurosci. 2016;19:517-22 pubmed publisher
Lv Q, Yuan L, Song Y, Sui T, Li Z, Lai L. D-repeat in the XIST gene is required for X chromosome inactivation. RNA Biol. 2016;13:172-6 pubmed publisher
Blassberg R, Macrae J, Briscoe J, Jacob J. Reduced cholesterol levels impair Smoothened activation in Smith-Lemli-Opitz syndrome. Hum Mol Genet. 2016;25:693-705 pubmed publisher
Durruthy Durruthy J, Sebastiano V, Wossidlo M, Cepeda D, Cui J, Grow E, et al. The primate-specific noncoding RNA HPAT5 regulates pluripotency during human preimplantation development and nuclear reprogramming. Nat Genet. 2016;48:44-52 pubmed publisher
Yue F, Li W, Zou J, Chen Q, Xu G, Huang H, et al. Blocking the association of HDAC4 with MAP1S accelerates autophagy clearance of mutant Huntingtin. Aging (Albany NY). 2015;7:839-53 pubmed
Chatterjee S, Saj A, Gocha T, Murphy M, Gonsalves F, Zhang X, et al. Inhibition of β-catenin-TCF1 interaction delays differentiation of mouse embryonic stem cells. J Cell Biol. 2015;211:39-51 pubmed publisher
Jing J, He L, Sun A, Quintana A, Ding Y, Ma G, et al. Proteomic mapping of ER-PM junctions identifies STIMATE as a regulator of Ca²⁺ influx. Nat Cell Biol. 2015;17:1339-47 pubmed publisher
Courtney D, Moore J, Atkinson S, Maurizi E, Allen E, Pedrioli D, et al. CRISPR/Cas9 DNA cleavage at SNP-derived PAM enables both in vitro and in vivo KRT12 mutation-specific targeting. Gene Ther. 2016;23:108-12 pubmed publisher
Park H, Kim Y, Yu B, Moroishi T, Mo J, Plouffe S, et al. Alternative Wnt Signaling Activates YAP/TAZ. Cell. 2015;162:780-94 pubmed publisher
Yamada N, Hasegawa Y, Yue M, Hamada T, Nakagawa S, Ogawa Y. Xist Exon 7 Contributes to the Stable Localization of Xist RNA on the Inactive X-Chromosome. PLoS Genet. 2015;11:e1005430 pubmed publisher
Hashidate Yoshida T, Harayama T, Hishikawa D, Morimoto R, Hamano F, Tokuoka S, et al. Fatty acid remodeling by LPCAT3 enriches arachidonate in phospholipid membranes and regulates triglyceride transport. elife. 2015;4: pubmed publisher
Carey B, Finley L, Cross J, Allis C, Thompson C. Intracellular α-ketoglutarate maintains the pluripotency of embryonic stem cells. Nature. 2015;518:413-6 pubmed publisher
Ran F, Hsu P, Wright J, Agarwala V, Scott D, Zhang F. Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013;8:2281-2308 pubmed publisher
product information
Catalog Number :
48139
Product Name :
pSpCas9(BB)-2A-Puro (PX459)
article :
doi10.1038/nprot.2013.143
id7475
pubmed_id24157548
bacterial resistance :
Ampicillin
cloning :
backbonePX459
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth notes :
For more information on Zhang Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/zhang/.
growth strain :
NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.
origin :
37
pi :
alt_names
SpCas9-2A-Puro
Cas9
PX459
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5AgeI
promoterCbh
sequencing_primer_3
sequencing_primer_5TTTATGGCGAGGCGGCGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namehSpCas9-2A-Puro
shRNA_sequence
size6000
species
100
Synthetic
tags
locationN terminal on insert
tag3XFLAG
locationC terminal on insert
tag2A-Puro
resistance markers :
898
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA