This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA4/TO-Flag-H3.3
catalog :
47980
citations: 4
Reference
Furth N, Algranati D, Dassa B, Beresh O, Fedyuk V, Morris N, et al. H3-K27M-mutant nucleosomes interact with MLL1 to shape the glioma epigenetic landscape. Cell Rep. 2022;39:110836 pubmed publisher
Bockaj I, Martini T, de Camargo Magalhães E, Bakker P, Meeuwsen de Boer T, Armandari I, et al. The H3.3K27M oncohistone affects replication stress outcome and provokes genomic instability in pediatric glioma. PLoS Genet. 2021;17:e1009868 pubmed publisher
Yuan S, Natesan R, Sanchez Rivera F, Li J, Bhanu N, Yamazoe T, et al. Global Regulation of the Histone Mark H3K36me2 Underlies Epithelial Plasticity and Metastatic Progression. Cancer Discov. 2020;10:854-871 pubmed publisher
Huang C, Zhang Z, Xu M, Li Y, Li Z, Ma Y, et al. H3.3-H4 tetramer splitting events feature cell-type specific enhancers. PLoS Genet. 2013;9:e1003558 pubmed publisher
product information
Catalog Number :
47980
Product Name :
pcDNA4/TO-Flag-H3.3
article :
doi10.1371/journal.pgen.1003558
id7207
pubmed_id23754967
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA4/TO
backbone_mutation
backbone_origininvitrogen
backbone_size5078
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
tetracycline-inducible expression of human Flag tagged H3F3A
origin :
37
pi :
alt_names
H3.3A
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoterCMV promoter with tetracycline operator
sequencing_primer_3BGH reverse
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBRYLIB1, H3-3B, H3.3A, H3F3, H3F3A
geneH3-3A
id3020
genbank_ids
mutation288 bp T to C synonymous mutation
nameH3F3A
shRNA_sequence
size438
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
1692
tags :
High Copy
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA